Purification and Characterization of Geranylgeranylglyceryl Phosphate Synthase from a Thermoacidophilic Archaeon, Thermoplasma acidophilum

2003 ◽  
Vol 133 (5) ◽  
pp. 651-657 ◽  
Author(s):  
N. Nemoto
Keyword(s):  
Purification And Characterization ◽  
Thermoplasma Acidophilum ◽  
Phosphate Synthase

scholarly journals Purification and characterization of N-acetylneuraminic acid-9-phosphate synthase from rat liver

Glycobiology ◽  
2002 ◽  
Vol 12 (2) ◽  
pp. 65-71 ◽  
Author(s):  
H. Chen ◽  
A. Blume ◽  
M. Zimmermann-Kordmann ◽  
W. Reutter ◽  
S. Hinderlich
Keyword(s):  
Rat Liver ◽  
Purification And Characterization ◽  
Acetylneuraminic Acid ◽  
Phosphate Synthase

Purification and characterization of Mycobacterium tuberculosis indole-3-glycerol phosphate synthase

2006 ◽  
Vol 71 (S1) ◽  
pp. S38-S43 ◽  
Author(s):  
Yanping Yang ◽  
Min Zhang ◽  
Hongmei Zhang ◽  
Jianqiang Lei ◽  
Ruiliang Jin ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Glycerol Phosphate ◽  
Purification And Characterization ◽  
Phosphate Synthase

scholarly journals Purification and characterization of glucose dehydrogenase from the thermoacidophilic archaebacterium Thermoplasma acidophilum

1989 ◽  
Vol 261 (3) ◽  
pp. 973-977 ◽  
Author(s):  
L D Smith ◽  
N Budgen ◽  
S J Bungard ◽  
M J Danson ◽  
D W Hough
Keyword(s):  
Amino Acid ◽  
Polypeptide Chain ◽  
Glucose Dehydrogenase ◽  
Sulfolobus Solfataricus ◽  
Purification And Characterization ◽  
Terminal Amino Acid ◽  
Thermoplasma Acidophilum ◽  
Catalytically Active ◽  
Terminal Amino

Glucose dehydrogenase was purified to homogeneity from the thermoacidophilic archaebacterium Thermoplasma acidophilum. The enzyme is a tetramer of polypeptide chain Mr 38,000 +/- 3000, it is catalytically active with both NAD+ and NADP+ cofactors, and it is thermostable and remarkably resistant to a variety of organic solvents. The amino acid composition was determined and compared with those of the glucose dehydrogenases from the archaebacterium Sulfolobus solfataricus and the eubacteria Bacillus subtilis and Bacillus megaterium. The N-terminal amino acid sequence of the Thermoplasma acidophilum enzyme was determined to be: (S/T)-E-Q-K-A-I-V-T-D-A-P-K-G-G-V-K-Y-T-T-I-D-M-P-E.

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