Systemic Insecticides and a Nuclear Polyhedrosis Virus for Control of the Bollworm and Tobacco Budworm on Cotton123

1976 ◽  
Vol 69 (5) ◽  
pp. 697-701 ◽  
Author(s):  
R. E. Kinzer ◽  
L. A. Bariola ◽  
R. L. Ridgway ◽  
S. L. Jones
Keyword(s):  
Nuclear Polyhedrosis Virus ◽  
Tobacco Budworm ◽  
Systemic Insecticides ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

In vivo Production of a Nuclear Polyhedrosis Virus Utilizing Tobacco Budworm and a Multicellular Larval Rearing Container2

1991 ◽  
Vol 26 (1) ◽  
pp. 69-75 ◽  
Author(s):  
M. R. Bell
Keyword(s):  
Heliothis Virescens ◽  
Inclusion Bodies ◽  
Nuclear Polyhedrosis Virus ◽  
Heliothis Armigera ◽  
Tobacco Budworm ◽  
Larval Rearing ◽  
Average Production ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

The effects of viral inoculum and incubation period on virus yield in a method of in vivo production of the multicapsid nuclear polyhedrosis virus of Heliothis armigera (Hübner) were examined utilizing Heliothis virescens larvae reared in multicellular containers. Trays of diet were surface contaminated with virus levels ranging from 54 to 2708 polyhedral inclusion bodies (PIB) per mm2 of diet at 7 days after egg infestation. The virus was harvested either at 6 or 7 days after treatment. Within the ranges of this study, the greatest quantity virus was obtained when the diet was inoculated with the 54 PIB level, and the virus was harvested at 7 days. This combination resulted in an average production of 2919 × 109 PIB per tray, with 5.7 × 109 PIB per larvae and 523 collectable larvae per tray. It also represented an increase in polyhedra of 2.43 × 105× compared to the inoculum. The costs of the production were estimated based on prevailing wages and current costs of materials.

Susceptibility of Five Plant Defoliators to Celery Looper (Lepidoptera: Noctuidae) Nuclear Polyhedrosis Virus

1996 ◽  
Vol 31 (3) ◽  
pp. 306-314
Author(s):  
Walter Solomon ◽  
Lavone Lambert ◽  
M. R. Bell
Keyword(s):  
Helicoverpa Zea ◽  
Nuclear Polyhedrosis Virus ◽  
Relative Activity ◽  
Corn Earworm ◽  
Tobacco Budworm ◽  
Beet Armyworm ◽  
Anticarsia Gemmatalis ◽  
Velvetbean Caterpillar ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

Bioassays were conducted to determine the susceptibility of five lepidopteran defoliators to a nuclear polyhedrosis virus (NPV) isolated from the celery looper, Anagrapha falcifera (Kirby). Tobacco budworm, Heliothis virescens (F.), exhibited the greatest susceptibility, LC50 = 2,328PIB/ml, and velvetbean caterpillar, Anticarsia gemmatalis (Hübner), exhibited the least susceptibility, LC50 = 5,176,038 PIB/ml, after 7 d of feeding at the 5 × 104 PIB/ml dosage. Corn earworm, Helicoverpa zea (Boddie), soybean looper, Pseudoplusia includens (Walker), and beet armyworm, Spodoptera exigua (Hübner), expressed intermediate levels of susceptibility, LC50 - 11,742, 14,195, 14,614 respectively, after 7 d of feeding at the 5 × 104 PIB/ml dosage. These results were consistent at days 10 and 14 indicating the relative activity of the virus against each species.

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