In vivo Production of a Nuclear Polyhedrosis Virus Utilizing Tobacco Budworm and a Multicellular Larval Rearing Container2

1991 ◽  
Vol 26 (1) ◽  
pp. 69-75 ◽  
Author(s):  
M. R. Bell
Keyword(s):  
Heliothis Virescens ◽  
Inclusion Bodies ◽  
Nuclear Polyhedrosis Virus ◽  
Heliothis Armigera ◽  
Tobacco Budworm ◽  
Larval Rearing ◽  
Average Production ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

The effects of viral inoculum and incubation period on virus yield in a method of in vivo production of the multicapsid nuclear polyhedrosis virus of Heliothis armigera (Hübner) were examined utilizing Heliothis virescens larvae reared in multicellular containers. Trays of diet were surface contaminated with virus levels ranging from 54 to 2708 polyhedral inclusion bodies (PIB) per mm2 of diet at 7 days after egg infestation. The virus was harvested either at 6 or 7 days after treatment. Within the ranges of this study, the greatest quantity virus was obtained when the diet was inoculated with the 54 PIB level, and the virus was harvested at 7 days. This combination resulted in an average production of 2919 × 109 PIB per tray, with 5.7 × 109 PIB per larvae and 523 collectable larvae per tray. It also represented an increase in polyhedra of 2.43 × 105× compared to the inoculum. The costs of the production were estimated based on prevailing wages and current costs of materials.

scholarly journals A Nuclear-Polyhedrosis Virus of Spodoptera frugiperda Isolated in Puerto Rico.

1969 ◽  
Vol 63 (2) ◽  
pp. 162-169
Author(s):  
Goro Kuno
Keyword(s):  
Puerto Rico ◽  
Spodoptera Frugiperda ◽  
Inclusion Bodies ◽  
Nuclear Polyhedrosis Virus ◽  
Galleria Mellonella ◽  
Fat Body ◽  
Heliothis Zea ◽  
Fourth Instar ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

A nuclear-polyhedrosis virus was isolated from a larva of Spodoptera frugiperda collected in Puerto Rico. The virus was found to be pathogenic to the larvae of Heliothis zea and of S. frugiperda but nonpathogenic to those of Diatraea saccharalis and of Galleria mellonella. LD50 for the fourth instar larvae of H. zea and S. frugiperda inoculated per os were 1.25 x 103 and 2.7 x 103 polyhedral inclusion bodies, respectively. The tissues infected included hemocytes, fat body, muscle, and epidermis. Furthermore, transovarian transmission of the virus was found in the inoculated individuals of S. frugiperda.

NUCLEAR POLYHEDROSIS VIRUS TRANSMISSION BY MICROPLITIS CROCEIPES (HYMENOPTERA: BRACONIDAE) ADULT FEMALES REARED IN INFECTED HELIOTHIS VIRESCENS (LEPIDOPTERA: NOCTUIDAE) LARVAE1,2

1989 ◽  
Vol 24 (4) ◽  
pp. 500-506 ◽  
Author(s):  
S. Y. Young ◽  
W. C. Yearian
Keyword(s):  
Heliothis Virescens ◽  
Nuclear Polyhedrosis Virus ◽  
Virus Transmission ◽  
Microplitis Croceipes ◽  
Control Group ◽  
Single Plant ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Lepidoptera Noctuidae ◽  
Cage Test

Heliothis nuclear polyhedrosis virus (HNPV) transmission by Microplitis croceipes (Cresson) [Hymenoptera: Braconidae] that emerged from HNPV-infected Heliothis virescens (F.) (Lepidoptera: Noctuidae) larvae was investigated in the laboratory and in single plant cage tests on field grown soybean. In the laboratory a small percentage of the M croceipes adults (13.3%) from infected larvae transmitted Heliothis HNPV. The mortality from HNPV in larvae exposed as late second instars to female parasitoids was 20.0%. In a single plant cage test on soybean conducted in the field, however, the percentage of mortality from HNPV in larvae did not differ significantly from that in the control group. When larvae were caged with females that had been artificially contaminated with HNPV up to 79.2% of the H. virescens larvae died of HNPV.

Preliminary spray trials of a nuclear polyhedrosis virus as a control agent for the brown-tail moth, Euproctis chrysorrhoea (L.) (Lepidoptera: Lymantriidae)

1988 ◽  
Vol 78 (2) ◽  
pp. 227-234 ◽  
Author(s):  
P. M. Kelly ◽  
P. H. Sterling ◽  
M. R. Speight ◽  
P. F. Entwistle
Keyword(s):  
Inclusion Bodies ◽  
Nuclear Polyhedrosis Virus ◽  
Field Trials ◽  
Control Agent ◽  
Fourth Instar ◽  
Considerable Potential ◽  
Low Volume ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
Euproctis Chrysorrhoea

AbstractA spray trial of a nuclear polyhedrosis virus (NPV) as an insecticide for Euproctis chrysorrhoea (L.) is described. The trial was carried out indoors in the spring on third-instar larvae emerging from overwintering nests onto small potted hawthorn trees (Crataegus sp.); the equipment used was the Turbair Fox Ultra Low Volume Sprayer. Dosages applied ranged from equivalents of 1 × 1011 to 1 × 1013 polyhedral inclusion bodies (PIB) per hectare. Infection was first detected in samples of live larvae after ten days and indicated rates of infection consistently lower than the final mortality attained. Larvae began to die of NPV infections 11 days after spraying, and mortality due to the virus ceased after ca 20 days; most larvae died in the fourth instar. Over the range of virus doses from 1 × 1011 to 5 ×10 12 PIB/ha mortality levels rose with dose, to a maximum of over 90%. Increasing the dosage to 1 × 1013 PIB/ha elicited no further increase in mortality. This preliminary trial suggests that the virus has considerable potential as a control agent, and field trials are, therefore, in progress.

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