The single cell gel electrophoresis assay for induced DNA damage (comet assay): measurement of tail length and moment

Mutagenesis ◽  
1995 ◽  
Vol 10 (2) ◽  
pp. 85-90 ◽  
Author(s):  
J. Ashby ◽  
H. Tinwell ◽  
P.A. Lefevre ◽  
M.A. Browne
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Tail Length ◽  
Single Cell Gel Electrophoresis

scholarly journals Can single cell gel electrophoresis [comet assay] predict the outcome in neonatal sepsis?

2018 ◽  
Vol 7 (03) ◽  
pp. 153-156
Author(s):  
Priyadharshini NA ◽  
Parkash Chand ◽  
Vishnu Bhatt S.
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Neonatal Sepsis ◽  
Gel Electrophoresis ◽  
Area Under The Curve ◽  
Tail Length ◽  
Single Cell Gel Electrophoresis ◽  
High Area ◽  
Predicting Outcome

Abstract Background & aim : Early diagnosis of neonatal sepsis is important for effective management and recovery. The available methods for diagnosis were not useful in predicting outcome. In the present study the technique of single cell gel electrophoresis [comet assay] which measures DNA damage was used to predict the outcome of neonatal sepsis. Material & methods : Comet Assay [single cell gel electrophoresis, SCGE] was used as a tool to assess the DNA damage in 3 5 term neonates with sepsis .The comet parameters were compared between those who recovered and expired due to sepsis neonatorum. Results were analysed using independent student t test with SPSS software version 19. p values <0.05 was taken as significant. Results : Comet length [174.57 ± 46.66pm] and Tail length [ 114.63 ± 52.92pm] are the prime indicator of DNA damage and were significantly [p Z < .05 ] higher among the expired cases when compared to the recovered cases [113.70 ±32.72 and 46.40±37.57 respectively]. Receiver Operating Characteristic [ROC] curve for comet length and tail length showed comet length and tail length are high. Area under the curve [AUC] 0.867 with cut off value of 140.06pm [71.4% sensitivity, 85.7% specificity] and 0.862 with cut off value of 63.12pm [85% sensitivity, 71.4% specificity] respectively. Conclusion :Weconclude that SCGE can be used for predicting mortality in neonatal sepsis

scholarly journals Inhibition of H2O2-induced DNA damage in single cell gel electrophoresis assay (comet assay) by castasterone isolated from leaves of centella asiatica

Health ◽  
2010 ◽  
Vol 02 (06) ◽  
pp. 595-602 ◽  
Author(s):  
Nishi Sondhi ◽  
Renu Bhardwaj ◽  
Satwinderjeet Kaur ◽  
Madhu Chandel ◽  
Neeraj Kumar ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Centella Asiatica ◽  
Single Cell Gel Electrophoresis

DNA damage measured by the single-cell gel electrophoresis (Comet) assay in mammals fed with mussels contaminated by the ‘Erika’ oil-spill

2005 ◽  
Vol 581 (1-2) ◽  
pp. 11-21 ◽  
Author(s):  
Sébastien Lemiere ◽  
Carole Cossu-Leguille ◽  
Antonio Bispo ◽  
Marie-José Jourdain ◽  
Marie-Claire Lanhers ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Oil Spill ◽  
Gel Electrophoresis ◽  
Single Cell Gel Electrophoresis
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