ASSESSMENT OF THE OCCLUSAL RELATIONS NATURE AT THE STAGES OF ORTHOPEDIC REHABILITATION OF PERSONS WITH PERIODONTAL TISSUE DISEASES COMPLICATED BY DENTITION DEFECTS

Resume. According to epidemiological studies, there is still an increased prevalence of periodontal disease among the population of different regions of the world, with increasing intensity and aggressiveness. Long-term chronic generalized lesions of recurrent and progressive inflammatory-dystrophic process lead to the destruction of alveolar processes, pathological mobility of teeth, tooth loss and dentofacial dysfunctions. One of the most characteristic symptoms of generalized periodontitis is the presence of traumatic occlusion. Depending on the degree of pathological changes in periodontal tissues, the size and topography of dentition defects, the replacement of dentition pathologies should be planned by splint-type orthopedic structures and computerized analysis of occlusal relationships with T-Scan III system. The aim of the study is to improve the effectiveness of orthopedic treatment of periodontal diseases complicated by defects of the dentition with the control of occlusal relationships with a computerized T-Scan III system. Materials and methods. We have examined 87 persons aged between 30 and 69 years with generalized periodontitis of I – III severity, complicated by partial loss of teeth of different sizes and topography, and a control group of the same age in 10 patients with intact dentition and physiological forms of occlusion without clinically diagnosed periodontal diseases. Computerized analysis of occlusion indices was performed by the T-Scan III system. We have investigated the stereotype of functional loads according to the following quantitative and qualitative indicators: the index of asymmetry of force between the parts (%); time of occurrence of the maximum amount of dental contacts (sec); time of disclusion (sec); the presence of premature occlusal contacts on the natural teeth and orthopedic constructions, and the presence of changes in the direction of the trajectory of the total vector of occlusal load. Orthopedic rehabilitation of patients in the case of periodontal diseases of the I-II degree of severity and the presence of small defects of the dentition, we conducted by splint-type orthopedic structures, dental implants, and artificial crowns of our own design with discrete occlusal loading (Licence for Effective Model No 143278, Ukraine, MPK А61С 13/007, u 2019 11487: Artificial Crown with Support on a Dental Implant). Patients with periodontal diseases of the II – III severity, complicated by defects of dentitions with different size and topography, the dental prosthetics has been performed by traditional removable and combined orthopedic splint-type constructions, and removable splint-type dentures of their own design: "Removable Prosthesis of Splint Type" (Licence for Effective Model No 130980 Ukraine, IPC (2006.01) А61С 13/007, u 2018 03039, 10.01.2019). Computerized analysis of occlusion indicators for the patients of I and II clinical groups and the III control group has been performed before and after orthopedic treatment by T-Scan III system. Results of the research. The results of investigation for functional occlusion have demonstrated that the index of relative strength asymmetry between the sides of the dentition in patients with generalized periodontitis complicated by dentition defects increased significantly and led to nonphysiological redistribution of loads compared with indices obtained in patients from control group due to the lack of correspondence for occlusal contacts. These indices are equal to 16.7 ± 3.8% in patients of I group and 18.9 ± 4.8% in group II compared to the similar control indicators – 4.7 ± 1.01%, respectively (p≤0.01). It should be noted that these indicators have differed from the control group indicators according to the severity of the pathological process. Rational prosthetics with removable and non-removable orthopedic structures, including on the base of dental implants, helps to optimize occlusal relations. Conclusions. Occlusographic studies performed in patients with generalized periodontitis having different severity and complicated by partial tooth loss have showed increased index of relative strength asymmetry between the sides of chewing, the presence of premature contacts and incorrect location of the trajectory of the total occlusal load vector. The dynamics of pathological changes in occlusion indices corresponds to the degree of generalized periodontitis, which indicates the necessity of opportune replacement of dentition defects, taking into account the occlusal relations. Rational prosthetics helps to optimize occlusal relations.

Characteristic pancreatic and splenic immune cell infiltration patterns in mouse acute pancreatitis

Background A systemic evaluation of immune cell infiltration patterns in experimental acute pancreatitis (AP) is lacking. Using multi-dimensional flow cytometry, this study profiled infiltrating immune cell types in multiple AP mouse models. Methods Three AP models were generated in C57BL/6 mice via cerulein (CAE) injection, alcohol and palmitoleic acid (EtOH + POA) injection, and alcohol diet feeding and cerulein (EtOH + CAE) injection. Primary pancreatic cells and splenocytes were prepared, and multi-dimensional flow cytometry was performed and analyzed by manual gating and computerized PhenoGraph, followed by visualization with t-distributed stochastic neighbor embedding (t-SNE). Results CAE treatment induced a time-dependent increase of major innate immune cells and a decrease of follicular B cells, and TCD4+ cells and the subtypes in the pancreas, whereas elicited a reversed pattern in the spleen. EtOH + POA treatment resulted in weaker effects than CAE treatment. EtOH feeding enhanced CAE-induced amylase secretion, but unexpectedly attenuated CAE-induced immune cell regulation. In comparison with manual gating analysis, computerized analysis demonstrated a remarkable time efficiency and reproducibility on the innate immune cells and B cells. Conclusions The reverse pattern of increased innate and decreased adaptive immune cells was consistent in the pancreas in CAE and EtOH + POA treatments. Alcohol feeding opposed the CAE effect on immune cell regulation. Together, the immune profiling approach utilized in this study provides a better understanding of overall immune responses in AP, which may facilitate the identification of intervention windows and new therapeutic strategies. Computerized analysis is superior to manual gating by dramatically reducing analysis time.

Chickpea seed vigor evaluated by computerized seedling analysis

Computerized seedling analysis is a promising tool for assessing seed vigor, providing reliable and less subjective results. The aim of this study was to define a method for evaluating the vigor of chickpea seeds through computerized image analysis, using the Seedling Analysis System (Sistema de Análise de Plântula - SAPL®) as a basis. Two chickpea cultivars were evaluated, consisting of five seed lots of cv. BRS Aleppo and four seed lots of cv. Cícero. Test were performed on the seeds of each cultivar for initial characterization of the physiological potential of the lots, and the results were compared with the determinations made via SAPL®. The results showed high correlations between the variables obtained from computerized analysis and from traditional vigor tests, especially for the close relationship between the cold test and low temperature germination test with the corrected vigor index (r > 0.9) evaluated on the fourth day after the beginning of the test. The total length of seedlings, length of the primary root, and growth and corrected vigor indices obtained through computerized analysis of seedlings on the 4th day after sowing allowed separation of the lots regarding vigor. Computerized analysis of seedlings using SAPL® is effective in determining the vigor of chickpea seed lots.

Localization of epileptic seizure focus by computerized analysis of fMRI recordings

By computerized analysis of cortical activity recorded via fMRI for pediatric epilepsy patients, we implement algorithmic localization of epileptic seizure focus within one of eight cortical lobes. Our innovative machine learning techniques involve intensive analysis of large matrices of mutual information coefficients between pairs of anatomically identified cortical regions. Drastic selection of pairs of regions with biologically significant inter-connectivity provides efficient inputs for our multi-layer perceptron (MLP) classifier. By imposing rigorous parameter parsimony to avoid overfitting, we construct a small-size MLP with very good percentages of successful classification.