scholarly journals Characterization of a segmented double-stranded RNA virus inDrosophilaKccells

1983 ◽  
Vol 11 (11) ◽  
pp. 3665-3678 ◽  
Author(s):  
Julia T. Hsu ◽  
Marilyn M. Sanders
2011 ◽  
Vol 155 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Haruhiko Isawa ◽  
Ryusei Kuwata ◽  
Keita Hoshino ◽  
Yoshio Tsuda ◽  
Kouji Sakai ◽  
...  

2013 ◽  
Vol 94 (6) ◽  
pp. 1426-1434 ◽  
Author(s):  
Diego F. Quito-Avila ◽  
Philip M. Brannen ◽  
William O. Cline ◽  
Philip F. Harmon ◽  
Robert R. Martin

A new disorder was observed on southern highbush blueberries in several south-eastern states in the USA. Symptoms included irregularly shaped circular spots or blotches with green centres on the upper and lower surfaces of leaves. Double-stranded RNA was extracted from symptomatic leaves suggesting the presence of virus(es) possibly involved in the disease. Sequencing revealed the presence of a novel RNA virus with a ~14 kb genome divided into four RNA segments. Sequence analyses showed that the virus, for which we propose the name Blueberry necrotic ring blotch virus (BNRBV), possesses protein domains conserved across RNA viruses in the alpha-virus-like supergroup. Phylogenetic inferences using different genes placed BNRBV in a clade that includes the Bromoviridae, the genus Cilevirus (CiLV) and the recently characterized Hibiscus green spot virus (HGSV). Despite the strong genetic relationships found among BNRBV, Cilevirus and HGSV, the genome of BNRBV contains three features that distinguish it significantly from its closest relatives: (i) the presence of two helicase domains with different evolutionary pathways, (ii) the existence of three conserved nucleotide stretches located at the 3′ non-coding regions of each RNA segment and (iii) the conservation of terminal nucleotide motifs across each segment. Furthermore, CiLV and HGSV possess poly(A)-tailed bipartite and tripartite genomes, respectively, whereas BNRBV has a quadra-partite genome lacking a poly(A) tail. Based on these genetic features a new genus is proposed for the classification of BNRBV.


Virus Genes ◽  
2020 ◽  
Vol 56 (2) ◽  
pp. 236-248 ◽  
Author(s):  
Lumi Viljakainen ◽  
Anna-Maria Borshagovski ◽  
Sami Saarenpää ◽  
Arja Kaitala ◽  
Jaana Jurvansuu

AbstractThe common glow-worms (Lampyris noctiluca) are best known for emission of green light by their larvae and sexually active adult females. However, both their DNA and RNA viruses remain unknown. Glow-worms are virologically interesting, as they are non-social and do not feed as adults, and hence their viral transmission may be limited. We identified viral sequences from 11 different virus taxa by the RNA-sequencing of two Finnish populations of adult glow-worms. The viruses represent nine different virus families and have negative, positive, or double-stranded RNA genomes. We also found a complete retroviral genome. Similar viral sequences were found from the sequencing data of common eastern firefly of North America, a species belonging to the same family (Lampyridae) as that of the common glow-worm. On average, an individual glow-worm had seven different RNA virus types and most of them appeared to establish a stable infection since they were found from glow-worms during two consecutive years. Here we present the characterization of load, prevalence, and interactions for each virus. Most of the glow-worm RNA viruses seem to be transmitted vertically, which may reflect the biology of glow-worms as non-social capital breeders, i.e., they invest stored resources in reproduction.


Biochemistry ◽  
2002 ◽  
Vol 41 (40) ◽  
pp. 11946-11953 ◽  
Author(s):  
James M. Benevides ◽  
Jarmo T. Juuti ◽  
Roman Tuma ◽  
Dennis H. Bamford ◽  
George J. Thomas

2008 ◽  
Vol 30 (4) ◽  
pp. 604-613 ◽  
Author(s):  
S.F. Shamoun ◽  
A. Varga ◽  
R.A. Valverde ◽  
T. Ramsfield ◽  
G. Sumampong ◽  
...  

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