scholarly journals The enhancer factor R of Epstein-Barr virus (EBV) Is a sequence-specific DNA binding protein

1990 ◽  
Vol 18 (23) ◽  
pp. 6835-6843 ◽  
Author(s):  
Henri Gruffat ◽  
Evelyne Manet ◽  
Agnès Rigolet ◽  
Alain Sergeant
Keyword(s):  
Dna Binding ◽  
Epstein Barr Virus ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Barr Virus ◽  
Epstein Barr ◽  
Enhancer Factor

Structural features of the single-stranded DNA-binding protein of Epstein–Barr virus

2008 ◽  
Vol 161 (2) ◽  
pp. 172-187 ◽  
Author(s):  
E. Mumtsidu ◽  
A.M. Makhov ◽  
P.V. Konarev ◽  
D.I. Svergun ◽  
J.D. Griffith ◽  
...  
Keyword(s):  
Dna Binding ◽  
Epstein Barr Virus ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Structural Features ◽  
Single Stranded Dna ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Epstein–Barr virus nuclear antigen 1 is a DNA-binding protein with strong RNA-binding activity

2004 ◽  
Vol 85 (10) ◽  
pp. 2755-2765 ◽  
Author(s):  
Chih-Chung Lu ◽  
Chia-Wei Wu ◽  
Shin C. Chang ◽  
Tzu-Yi Chen ◽  
Chwan-Ren Hu ◽  
...  
Keyword(s):  
Dna Binding ◽  
Epstein Barr Virus ◽  
Rna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Binding Activity ◽  
Nuclear Antigen ◽  
Barr Virus ◽  
Infected Cells ◽  
Epstein Barr

Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA-1) plays key roles in both the regulation of gene expression and the replication of the EBV genome in latently infected cells. To characterize the RNA-binding activity of EBNA-1, it was demonstrated that EBNA-1 binds efficiently to RNA homopolymers that are composed of poly(G) and weakly to those composed of poly(U). All three RGG boxes of EBNA-1 contributed additively to poly(G)-binding activity and could mediate RNA binding when attached to a heterologous protein in an RNA gel mobility-shift assay. In vitro-transcribed EBV and non-EBV RNA probes revealed that EBNA-1 bound to most RNAs examined and the affinity increased as the content of G and U increased, as demonstrated in competition assays. Among these probes, the 5′ non-coding region (NCR) (nt 131–278) of hepatitis C virus RNA appeared to be the strongest competitor for EBNA-1 binding to the EBV-encoded small nuclear RNA 1 (EBER1) probe, whereas a mutant 5′ NCR RNA with partially disrupted secondary structure was a weak competitor. Furthermore, the interaction of endogenous EBNA-1 and EBER1 in EBV-infected cells was demonstrated by a ribonucleoprotein immunoprecipitation assay. These results revealed that EBNA-1 is a DNA-binding protein with strong binding activity to a relatively broad spectrum of RNA and suggested an additional biological impact of EBNA-1 through its ability to bind to RNA.

scholarly journals Characterization of Epstein-Barr Virus DNase and Its Interaction with the Major DNA Binding Protein

Virology ◽  
1995 ◽  
Vol 208 (2) ◽  
pp. 712-722 ◽  
Author(s):  
Su-Fang Lin ◽  
Tsuey-Ying Hsu ◽  
Mei-Ying Liu ◽  
Lung-Shen Lin ◽  
Huey-Lang Yang ◽  
...  
Keyword(s):  
Dna Binding ◽  
Epstein Barr Virus ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Two Domains of the Epstein-Barr Virus Origin DNA-binding Protein, EBNA1, Orchestrate Sequence-specific DNA Binding

2000 ◽  
Vol 275 (29) ◽  
pp. 22273-22277 ◽  
Author(s):  
Jennifer Cruickshank ◽  
Kathy Shire ◽  
Alan R. Davidson ◽  
Aled M. Edwards ◽  
Lori Frappier
Keyword(s):  
Dna Binding ◽  
Epstein Barr Virus ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Barr Virus ◽  
Virus Origin ◽  
Epstein Barr

scholarly journals Epstein–Barr Virus Single-Stranded DNA-Binding Protein: Purification, Characterization, and Action on DNA Synthesis by the Viral DNA Polymerase

Virology ◽  
1996 ◽  
Vol 222 (2) ◽  
pp. 352-364 ◽  
Author(s):  
Tatsuya Tsurumi ◽  
Akio Kobayashi ◽  
Katsuyuki Tamai ◽  
Hiroshi Yamada ◽  
Tohru Daikoku ◽  
...  
Keyword(s):  
Dna Binding ◽  
Dna Synthesis ◽  
Protein Purification ◽  
Dna Polymerase ◽  
Epstein Barr Virus ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Viral Dna ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Crystal Structure of the DNA-Binding Domain of the Epstein–Barr Virus Origin-Binding Protein, EBNA1, Bound to DNA

Cell ◽  
1996 ◽  
Vol 84 (5) ◽  
pp. 791-800 ◽  
Author(s):  
Alexey Bochkarev ◽  
Jean A Barwell ◽  
Richard A Pfuetzner ◽  
Elena Bochkareva ◽  
Lori Frappier ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Binding ◽  
Epstein Barr Virus ◽  
Binding Protein ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Barr Virus ◽  
Virus Origin ◽  
Origin Binding Protein ◽  
Epstein Barr

scholarly journals Crystal structure of the DNA-binding domain of the Epstein-Barr virus origin-binding protein EBNA1

Cell ◽  
1995 ◽  
Vol 83 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Alexey Bochkarev ◽  
Jean A. Barwell ◽  
Richard A. Pfuetzner ◽  
William Furey ◽  
Aled M. Edwards ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Dna Binding ◽  
Epstein Barr Virus ◽  
Binding Protein ◽  
Binding Domain ◽  
Dna Binding Domain ◽  
Barr Virus ◽  
Virus Origin ◽  
Origin Binding Protein ◽  
Epstein Barr
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