scholarly journals Consensus inverted terminal repeat sequence ofParameciumlESs: resemblance to termini of Tc1 -related andEuplotesTec transposons

1995 ◽  
Vol 23 (11) ◽  
pp. 2006-2013 ◽  
Author(s):  
Lawrence A. Klobutcher ◽  
Glenn Herrick
2020 ◽  
Vol 31 (3-4) ◽  
pp. 151-162 ◽  
Author(s):  
Lauriel F. Earley ◽  
Laura M. Conatser ◽  
Victoria M. Lue ◽  
Amanda L. Dobbins ◽  
Chengwen Li ◽  
...  

Gene ◽  
1980 ◽  
Vol 10 (4) ◽  
pp. 301-306 ◽  
Author(s):  
Oka Yoshio ◽  
Shiota Susumu ◽  
Nakai Sumiko ◽  
Nishida Yasuyoshi ◽  
Okubo Shunzo

2014 ◽  
Vol 95 (7) ◽  
pp. 1574-1584 ◽  
Author(s):  
Kerstin Wunderlich ◽  
Esmeralda van der Helm ◽  
Dirk Spek ◽  
Mark Vermeulen ◽  
Adile Gecgel ◽  
...  

During the development of human adenovirus 35-derived replication-incompetent (rAd35) vaccine vectors for prevention of infectious diseases, we detected mutations in the terminal 8 nt of the inverted terminal repeats (ITRs) of rAd35. The switch from the plasmid-encoded sequence 5′-CATCATCA-3′ to the alternative sequence 5′-CTATCTAT-3′ in the ITRs was found to be a general in vitro propagation phenomenon, as shown for several vectors carrying different transgenes or being derived from different adenovirus serotypes. In each tested case, the plasmid-encoded ITR sequence changed to exactly the same alternative ITR sequence, 5′-CTATCTAT-3′. The outgrowth of this alternative ITR version should result from a growth advantage conferred by the alternative ITR sequence. Indeed, replication kinetics studies of rAd35 harbouring either the original or alternative ITR sequence confirmed an increase in replication speed for rAd35 vectors with the alternative ITR sequence. These findings can be applied to generate recombinant adenoviral vectors harbouring the alternative ITR sequence, which will facilitate the generation of genetically homogeneous seed virus batches. Moreover, vector production may be accelerated by taking advantage of the observed improved replication kinetics associated with the alternative ITR sequence.


1987 ◽  
Vol 7 (3) ◽  
pp. 1063-1069
Author(s):  
M B Vasudevachari ◽  
V Natarajan ◽  
N P Salzman

Linear DNAs, containing a copy of the adenovirus serotype 2 (Ad2) inverted terminal repeat sequence at each end, replicate in 293 cells when cotransfected with Ad2 DNA (Hay et al., J. Mol. Biol. 175:493-510, 1984). We have linked either the Ad2 IVa2 promoter (IVa2) or major late promoter (MLP) to the chloramphenicol acetyltransferase gene and inserted this DNA into such a plasmid (pARKR) between its two inverted terminal repeats. These recombinant plasmids were linearized and then used to transfect 293 cells in the presence or absence of Ad2 helper DNA. Synthesis of IVa2 and MLP RNAs, and production of chloramphenicol acetyltransferase was increased dramatically when the Ad2 DNA was included. However, unlike the patterns of temporal regulation which are seen during a cycle of virus replication when these genes are contained within the virion, there was no obvious difference in the timing of RNA synthesis from plasmid IVa2 or MLP after cotransfection. When linearized plasmids containing IVa2 and MLP sequences but lacking inverted terminal repeats at their ends (replication deficient plasmids) were used for transfection, an increase in RNA synthesis from IVa2 or MLP was also observed and similarly required cotransfection with Ad2 DNA. When HeLa cells, which do not constitutively express the adenovirus E1a gene, were cotransfected with linearized plasmids and adenovirus DNA that lacks the E1a region (H5dl312), a stimulation of transcription was also observed, although it was less than the level observed with wild-type DNA. The results of the present study demonstrate that an early gene product(s) besides E1a functions in trans to regulate transcription.


2010 ◽  
Vol 26 (12) ◽  
pp. 1327-1331 ◽  
Author(s):  
Mariana Cavalheiro Magri ◽  
Helena Kaminami Morimoto ◽  
Luis Fernando de Macedo Brígido ◽  
Rosangela Rodrigues ◽  
Adele Caterino–de–Araujo

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