Long Terminal Repeat Sequence Analysis of HTLV-2 Molecular Variants Identified in Southern Brazil

2010 ◽  
Vol 26 (12) ◽  
pp. 1327-1331 ◽  
Author(s):  
Mariana Cavalheiro Magri ◽  
Helena Kaminami Morimoto ◽  
Luis Fernando de Macedo Brígido ◽  
Rosangela Rodrigues ◽  
Adele Caterino–de–Araujo
Keyword(s):  
Sequence Analysis ◽  
Long Terminal Repeat ◽  
Repeat Sequence ◽  
Terminal Repeat ◽  
Long Terminal Repeat Sequence ◽  
Southern Brazil ◽  
Molecular Variants ◽  
Terminal Repeat Sequence

scholarly journals Unregulated expression of the erythropoietin receptor gene caused by insertion of spleen focus-forming virus long terminal repeat in a murine erythroleukemia cell line.

1991 ◽  
Vol 11 (11) ◽  
pp. 5527-5533 ◽  
Author(s):  
M Hino ◽  
A Tojo ◽  
Y Misawa ◽  
H Morii ◽  
F Takaku ◽  
...  
Keyword(s):  
Long Terminal Repeat ◽  
Cell Line ◽  
Repeat Sequence ◽  
Terminal Repeat ◽  
Long Terminal Repeat Sequence ◽  
Erythroid Progenitor ◽  
Erythroleukemia Cell ◽  
Murine Erythroleukemia ◽  
Terminal Repeat Sequence ◽  
Murine Erythroleukemia Cell

A murine erythroleukemia (MEL) cell line, F5-5, expressed 10,000 binding sites for erythropoietin (EPO) per cell, 10-fold more than was expressed by other murine erythroleukemia cell lines and normal erythroid progenitors. Northern (RNA) and Southern blot analyses revealed overexpression of mRNA for the EPO receptor (EPOR) and rearrangement of one of the EPOR gene alleles in F5-5 cells, respectively. Molecular cloning of F5-5-derived cDNA encoding EPOR revealed that the 5' noncoding region of the EPOR cDNA corresponds to the 3' long terminal repeat sequence of the polycythemic strain of Friend spleen focus-forming virus (F-SFFVP). The aberrant EPOR transcripts containing the 3' long terminal repeat sequence were mainly expressed in F5-5 cells. The same integration upstream of the EPOR gene was also observed in other subclones and the parent cell line. It is possible that overexpression of EPOR by viral promoter insertion will confer growth advantage to an F-SFFVP-infected erythroid progenitor cell, leading to positive clonal selection through further leukemogenic steps.

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