scholarly journals Adeno-Associated Virus Serotype-Specific Inverted Terminal Repeat Sequence Role in Vector Transgene Expression

2020 ◽  
Vol 31 (3-4) ◽  
pp. 151-162 ◽  
Author(s):  
Lauriel F. Earley ◽  
Laura M. Conatser ◽  
Victoria M. Lue ◽  
Amanda L. Dobbins ◽  
Chengwen Li ◽  
...  
Keyword(s):  
Transgene Expression ◽  
Repeat Sequence ◽  
Terminal Repeat ◽  
Inverted Terminal Repeat ◽  
Adeno Associated Virus ◽  
Inverted Terminal Repeat Sequence ◽  
Virus Serotype ◽  
Terminal Repeat Sequence

Inverted terminal repeat sequence in the macronuclear DNA of Stylonychia pustulata

Gene ◽  
1980 ◽  
Vol 10 (4) ◽  
pp. 301-306 ◽  
Author(s):  
Oka Yoshio ◽  
Shiota Susumu ◽  
Nakai Sumiko ◽  
Nishida Yasuyoshi ◽  
Okubo Shunzo
Keyword(s):  
Repeat Sequence ◽  
Terminal Repeat ◽  
Inverted Terminal Repeat ◽  
Inverted Terminal Repeat Sequence ◽  
Terminal Repeat Sequence

Universal Real-Time PCR for the Detection and Quantification of Adeno-Associated Virus Serotype 2-Derived Inverted Terminal Repeat Sequences

2011 ◽  
pp. 110805114044008 ◽  
Author(s):  
Christine Aurnhammer ◽  
Maren Haase ◽  
Nadine Muether ◽  
Martin Hausl ◽  
Christina Rauschhuber ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Terminal Repeat ◽  
Inverted Terminal Repeat ◽  
Adeno Associated Virus ◽  
Repeat Sequences ◽  
Virus Serotype ◽  
Detection And Quantification

scholarly journals An alternative to the adenovirus inverted terminal repeat sequence increases the viral genome replication rate and provides a selective advantage in vitro

2014 ◽  
Vol 95 (7) ◽  
pp. 1574-1584 ◽  
Author(s):  
Kerstin Wunderlich ◽  
Esmeralda van der Helm ◽  
Dirk Spek ◽  
Mark Vermeulen ◽  
Adile Gecgel ◽  
...  
Keyword(s):  
Human Adenovirus ◽  
Selective Advantage ◽  
Adenoviral Vectors ◽  
Repeat Sequence ◽  
Inverted Terminal Repeat ◽  
Genome Replication ◽  
Inverted Terminal Repeat Sequence ◽  
Viral Genome Replication ◽  
Terminal Repeat Sequence

During the development of human adenovirus 35-derived replication-incompetent (rAd35) vaccine vectors for prevention of infectious diseases, we detected mutations in the terminal 8 nt of the inverted terminal repeats (ITRs) of rAd35. The switch from the plasmid-encoded sequence 5′-CATCATCA-3′ to the alternative sequence 5′-CTATCTAT-3′ in the ITRs was found to be a general in vitro propagation phenomenon, as shown for several vectors carrying different transgenes or being derived from different adenovirus serotypes. In each tested case, the plasmid-encoded ITR sequence changed to exactly the same alternative ITR sequence, 5′-CTATCTAT-3′. The outgrowth of this alternative ITR version should result from a growth advantage conferred by the alternative ITR sequence. Indeed, replication kinetics studies of rAd35 harbouring either the original or alternative ITR sequence confirmed an increase in replication speed for rAd35 vectors with the alternative ITR sequence. These findings can be applied to generate recombinant adenoviral vectors harbouring the alternative ITR sequence, which will facilitate the generation of genetically homogeneous seed virus batches. Moreover, vector production may be accelerated by taking advantage of the observed improved replication kinetics associated with the alternative ITR sequence.

Universal Real-Time PCR for the Detection and Quantification of Adeno-Associated Virus Serotype 2-Derived Inverted Terminal Repeat Sequences

2012 ◽  
Vol 23 (1) ◽  
pp. 18-28 ◽  
Author(s):  
Christine Aurnhammer ◽  
Maren Haase ◽  
Nadine Muether ◽  
Martin Hausl ◽  
Christina Rauschhuber ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Terminal Repeat ◽  
Inverted Terminal Repeat ◽  
Adeno Associated Virus ◽  
Repeat Sequences ◽  
Virus Serotype ◽  
Detection And Quantification

scholarly journals Rescue of the Adeno-Associated Virus Genome from a Plasmid Vector: Evidence for Rescue by Replication

2003 ◽  
Vol 77 (21) ◽  
pp. 11480-11490 ◽  
Author(s):  
Peter Ward ◽  
Per Elias ◽  
R. Michael Linden
Keyword(s):  
Cultured Cells ◽  
Plasmid Vector ◽  
Virus Genome ◽  
Helper Virus ◽  
Repeat Sequence ◽  
Inverted Terminal Repeat ◽  
Chromosome 19 ◽  
Adeno Associated Virus ◽  
Inverted Terminal Repeat Sequence ◽  
Simplex Virus

ABSTRACT In cultured cells, adeno-associated virus (AAV) replication requires coinfection with a helper virus, either adenovirus or herpesvirus. In the absence of helper virus coinfection AAV can integrate its genome site specifically into the AAVS1 region of chromosome 19. Upon subsequent infection with a helper virus, the AAV genome is released from chromosome 19 by a process termed rescue, and productive replication ensues. The AAV genome cloned into a plasmid vector can also serve to initiate productive AAV replication. When such constructs are transfected into cells and those cells are simultaneously or subsequently infected with a helper virus, the AAV genome is released from the plasmid. This process is thought to serve as a model for rescue from the human genomic site. In this report we present a model for rescue of AAV genomes by replication. A hallmark of this model is the production of a partially single-stranded and partially double-stranded molecule. We show that the AAV2 Rep 68 protein, together with the UL30/UL42 herpes simplex virus type 1 DNA polymerase and the UL29 single-strand DNA binding protein ICP8, is sufficient to efficiently and precisely rescue AAV from a plasmid in a way that is dependent on the AAV inverted terminal repeat sequence.

scholarly journals A novel 165-base-pair terminal repeat sequence is the sole cis requirement for the adeno-associated virus life cycle.

1997 ◽  
Vol 71 (2) ◽  
pp. 941-948 ◽  
Author(s):  
X Xiao ◽  
W Xiao ◽  
J Li ◽  
R J Samulski
Keyword(s):  
Life Cycle ◽  
Base Pair ◽  
Repeat Sequence ◽  
Terminal Repeat ◽  
Adeno Associated Virus ◽  
Virus Life Cycle ◽  
Terminal Repeat Sequence

scholarly journals Cotransfection with adenovirus DNA enhances transcription from linear DNA containing eucaryotic promoters.

1987 ◽  
Vol 7 (3) ◽  
pp. 1063-1069
Author(s):  
M B Vasudevachari ◽  
V Natarajan ◽  
N P Salzman
Keyword(s):  
Rna Synthesis ◽  
Chloramphenicol Acetyltransferase ◽  
Repeat Sequence ◽  
Early Gene ◽  
Inverted Terminal Repeat ◽  
Adenovirus E1a ◽  
293 Cells ◽  
Inverted Terminal Repeat Sequence ◽  
Inverted Terminal Repeats ◽  
Terminal Repeats

Linear DNAs, containing a copy of the adenovirus serotype 2 (Ad2) inverted terminal repeat sequence at each end, replicate in 293 cells when cotransfected with Ad2 DNA (Hay et al., J. Mol. Biol. 175:493-510, 1984). We have linked either the Ad2 IVa2 promoter (IVa2) or major late promoter (MLP) to the chloramphenicol acetyltransferase gene and inserted this DNA into such a plasmid (pARKR) between its two inverted terminal repeats. These recombinant plasmids were linearized and then used to transfect 293 cells in the presence or absence of Ad2 helper DNA. Synthesis of IVa2 and MLP RNAs, and production of chloramphenicol acetyltransferase was increased dramatically when the Ad2 DNA was included. However, unlike the patterns of temporal regulation which are seen during a cycle of virus replication when these genes are contained within the virion, there was no obvious difference in the timing of RNA synthesis from plasmid IVa2 or MLP after cotransfection. When linearized plasmids containing IVa2 and MLP sequences but lacking inverted terminal repeats at their ends (replication deficient plasmids) were used for transfection, an increase in RNA synthesis from IVa2 or MLP was also observed and similarly required cotransfection with Ad2 DNA. When HeLa cells, which do not constitutively express the adenovirus E1a gene, were cotransfected with linearized plasmids and adenovirus DNA that lacks the E1a region (H5dl312), a stimulation of transcription was also observed, although it was less than the level observed with wild-type DNA. The results of the present study demonstrate that an early gene product(s) besides E1a functions in trans to regulate transcription.

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