scholarly journals Stability of the unique anticodon loop conformation of E.coli tRNAfMet

1979 ◽  
Vol 7 (6) ◽  
pp. 1457-1467 ◽  
Author(s):  
Paul Wrede ◽  
Alexander Rich
1979 ◽  
Vol 76 (7) ◽  
pp. 3289-3293 ◽  
Author(s):  
P. Wrede ◽  
N. H. Woo ◽  
A. Rich

Author(s):  
James F. Hainfeld ◽  
Kyra M. Alford ◽  
Mathias Sprinzl ◽  
Valsan Mandiyan ◽  
Santa J. Tumminia ◽  
...  

The undecagold (Au11) cluster was used to covalently label tRNA molecules at two specific ribonucleotides, one at position 75, and one at position 32 near the anticodon loop. Two different Au11 derivatives were used, one with a monomaleimide and one with a monoiodacetamide to effect efficient reactions.The first tRNA labeled was yeast tRNAphe which had a 2-thiocytidine (s2C) enzymatically introduced at position 75. This was found to react with the iodoacetamide-Aun derivative (Fig. 1) but not the maleimide-Aun (Fig. 2). Reaction conditions were 37° for 16 hours. Addition of dimethylformamide (DMF) up to 70% made no improvement in the labeling yield. A high resolution scanning transmission electron micrograph (STEM) taken using the darkfield elastically scattered electrons is shown in Fig. 3.


2019 ◽  
Vol 294 (14) ◽  
pp. 5281-5291 ◽  
Author(s):  
Ha An Nguyen ◽  
Eric D. Hoffer ◽  
Christine M. Dunham
Keyword(s):  

2021 ◽  
Vol 64 (6) ◽  
pp. 3048-3058
Author(s):  
Tobias F. Fischer ◽  
Clara T. Schoeder ◽  
Tristan Zellmann ◽  
Jan Stichel ◽  
Jens Meiler ◽  
...  
Keyword(s):  

1994 ◽  
Vol 269 (51) ◽  
pp. 32221-32225
Author(s):  
S. Nakanishi ◽  
T. Ueda ◽  
H. Hori ◽  
N. Yamazaki ◽  
N. Okada ◽  
...  

2005 ◽  
Vol 280 (37) ◽  
pp. 32177-32183 ◽  
Author(s):  
Neil P. Johnson ◽  
Walter A. Baase ◽  
Peter H. von Hippel

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