scholarly journals Identification and characterization of the mitochondrial RNA polymerase and transcription factor in the fission yeast Schizosaccharomyces pombe

2011 ◽  
Vol 39 (12) ◽  
pp. 5119-5130 ◽  
Author(s):  
Hengyi Jiang ◽  
Wenxia Sun ◽  
Zhe Wang ◽  
Jing Zhang ◽  
Dongrong Chen ◽  
...  
1987 ◽  
Vol 65 (2) ◽  
pp. 173-182 ◽  
Author(s):  
Michael Goldenthal ◽  
James T. Nishiura

A DNA-dependent RNA polymerase was solubilized from sucrose gradient isolated, DNase-treated mitochrondria of Drosophila melanogaster. The isolated mitochondria were not detectably contaminated with nuclear DNA as shown by CsCl gradient centrifugation and polylysine Kieselguhr chromatography. The detergent-solubilized RNA polymerase was sensitive to rifampicin, resistant to α-amanitin, had an apparent molecular mass of about 60 kilodaltons, and displayed a tendency to aggregate, both in crude extracts or when purified. The mitochondrial RNA polymerase could be distinguished from nuclear RNA polymerases on the basis of size, salt optima, rifampicin sensitivity, and α-amanitin resistance.


FEBS Letters ◽  
2001 ◽  
Vol 503 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Ascensión Prieto-Martı́n ◽  
Julio Montoya ◽  
Francisco Martı́nez-Azorı́n

DNA Repair ◽  
2004 ◽  
Vol 3 (10) ◽  
pp. 1363-1374 ◽  
Author(s):  
Fuat K. Khasanov ◽  
Albina F. Salakhova ◽  
Olga V. Chepurnaja ◽  
Vladimir G. Korolev ◽  
Vladimir I. Bashkirov

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