scholarly journals Expression and Purification of Mitochondrial RNA Polymerase and Transcription Factor A from Drosophila melanogaster

2016 ◽  
pp. 199-210
Author(s):  
John P. Gajewski ◽  
Jamie J. Arnold ◽  
Tiina S. Salminen ◽  
Laurie S. Kaguni ◽  
Craig E. Cameron
Keyword(s):  
Transcription Factor ◽  
Drosophila Melanogaster ◽  
Rna Polymerase ◽  
Mitochondrial Rna ◽  
Expression And Purification ◽  
Mitochondrial Rna Polymerase

Isolation and characterization of a mitochondrial RNA polymerase from Drosophila melanogaster

1987 ◽  
Vol 65 (2) ◽  
pp. 173-182 ◽  
Author(s):  
Michael Goldenthal ◽  
James T. Nishiura
Keyword(s):  
Drosophila Melanogaster ◽  
Rna Polymerase ◽  
Nuclear Dna ◽  
Gradient Centrifugation ◽  
Mitochondrial Rna ◽  
Mitochondrial Rna Polymerase ◽  
Isolation And Characterization ◽  
Cscl Gradient ◽  
Nuclear Rna

A DNA-dependent RNA polymerase was solubilized from sucrose gradient isolated, DNase-treated mitochrondria of Drosophila melanogaster. The isolated mitochondria were not detectably contaminated with nuclear DNA as shown by CsCl gradient centrifugation and polylysine Kieselguhr chromatography. The detergent-solubilized RNA polymerase was sensitive to rifampicin, resistant to α-amanitin, had an apparent molecular mass of about 60 kilodaltons, and displayed a tendency to aggregate, both in crude extracts or when purified. The mitochondrial RNA polymerase could be distinguished from nuclear RNA polymerases on the basis of size, salt optima, rifampicin sensitivity, and α-amanitin resistance.

scholarly journals A study on the human mitochondrial RNA polymerase activity points to existence of a transcription factor B-like protein

FEBS Letters ◽  
2001 ◽  
Vol 503 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Ascensión Prieto-Martı́n ◽  
Julio Montoya ◽  
Francisco Martı́nez-Azorı́n
Keyword(s):  
Transcription Factor ◽  
Rna Polymerase ◽  
Polymerase Activity ◽  
Mitochondrial Rna ◽  
Factor B ◽  
Mitochondrial Rna Polymerase ◽  
Rna Polymerase Activity

scholarly journals The Yeast Mitochondrial RNA Polymerase and Transcription Factor Complex Catalyzes Efficient Priming of DNA Synthesis on Single-stranded DNA

2016 ◽  
Vol 291 (32) ◽  
pp. 16828-16839 ◽  
Author(s):  
Aparna Ramachandran ◽  
Divya Nandakumar ◽  
Aishwarya P. Deshpande ◽  
Thomas P. Lucas ◽  
Ramanagouda R-Bhojappa ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Rna Polymerase ◽  
Dna Synthesis ◽  
Mitochondrial Rna ◽  
Single Stranded Dna ◽  
Mitochondrial Rna Polymerase ◽  
Transcription Factor Complex
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