scholarly journals L‐type calcium current of isolated rat cardiac myocytes in experimental uraemia

2000 ◽  
Vol 15 (6) ◽  
pp. 791-798 ◽  
Author(s):  
Paul Donohoe ◽  
Aisling C. McMahon ◽  
Omal V. Walgama ◽  
Federica Bertaso ◽  
Mark E. C. Dockrell ◽  
...  
Keyword(s):  
Cardiac Myocytes ◽  
Calcium Current ◽  
Rat Cardiac Myocytes ◽  
Isolated Rat

scholarly journals Insulin-induced Recruitment of Glucose Transporter 4 (GLUT4) and GLUT1 in Isolated Rat Cardiac Myocytes

1997 ◽  
Vol 272 (11) ◽  
pp. 7085-7092 ◽  
Author(s):  
Yvan Fischer ◽  
Julia Thomas ◽  
Lidia Sevilla ◽  
Purificación Muñoz ◽  
Christoph Becker ◽  
...  
Keyword(s):  
Cardiac Myocytes ◽  
Glucose Transporter ◽  
Glucose Transporter 4 ◽  
Rat Cardiac Myocytes ◽  
Isolated Rat

Use of thapsigargin to study Ca2+ homeostasis in cardiac cells

1995 ◽  
Vol 15 (5) ◽  
pp. 341-349 ◽  
Author(s):  
Terry B. Rogers ◽  
Giuseppe Inesi ◽  
Robert Wade ◽  
W. J. Lederer
Keyword(s):  
Cardiac Myocytes ◽  
Calcium Current ◽  
Potent Inhibitor ◽  
Cell Voltage ◽  
Cardiac Cells ◽  
Heart Cells ◽  
Absolute Requirement ◽  
Rat Heart Myocytes ◽  
Rat Cardiac Myocytes

Several reports have documented that thapsigargin is a potent inhibitor of the SR Ca2+ ATPase isolated from cardiac or skeletal muscle. We have characterized the specificity of this agent in intact rat cardiac myocytes using cells maintained in the whole cell voltage clamp configuration. We have shown that thapsigargin decreases the magnitude of the Ca2+ transient and the twitch by about 80% while it slows the decay rate for these responses. These changes were not accompanied by any alterations in sarcolemmal currents or in the trigger Ca2+ generated by the inward calcium current. Taken together these results reveal that the action of thapsigargin is restricted to the SR Ca2+ ATPase in intact cardiac myocytes. Furthermore, it is demonstrated unambiguously that SR intracellular Ca2+ stores are an absolute requirement for the development of contractile tension in rat heart myocytes. It is shown that thapsigargin is a valuable probe to examine the importance of SR pools of Ca2+ and the role of the Ca2+ ATPase in intact myocytes as well as in genetically altered heart cells.

Correlation Between Direct ESR Spectroscopic Measurements and Electromechanical and Biochemical Assessments of Exogenous Free Radical Injury in Isolated Rat Cardiac Myocytes

1998 ◽  
Vol 24 (1) ◽  
pp. 121-131 ◽  
Author(s):  
Martine Courtois ◽  
Véronique Maupoil ◽  
Elisabeth Fantini ◽  
Isabelle Durot ◽  
Anne Javouhey-Donzel ◽  
...  
Keyword(s):  
Free Radical ◽  
Cardiac Myocytes ◽  
Spectroscopic Measurements ◽  
Rat Cardiac Myocytes ◽  
Isolated Rat
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