Recent Developments in Forensic DNA Technology

2020 ◽  
pp. 105-127
Author(s):  
Henry Erlich ◽  
Cassandra Calloway ◽  
Steven B. Lee
Keyword(s):  
Mitochondrial Dna ◽  
Dna Analysis ◽  
Massively Parallel Sequencing ◽  
Massively Parallel ◽  
Degraded Dna ◽  
Forensic Dna ◽  
Current Standard ◽  
Forensic Dna Analysis ◽  
Str Loci ◽  
Recent Developments

The current standard of forensic DNA analysis is genotyping the length polymorphism of STR loci by capillary electrophoresis and analyzing the polymorphism of mitochondrial DNA by Sanger sequencing. However, the trend of dramatic technological developments begun in the mid 1980s has continued, with the most consequential recent innovations being (1) the development of next generation sequencing (NGS) or massively parallel sequencing (MPS) and (2) the implementation of commercial Rapid DNA instruments that automate genotyping of all CODIS core STR loci from sample to profile in 90 minutes. This chapter reviews the principles, benefits, and applications of NGS or MPS technology, with a focus on the critical features of massively parallel and clonal sequencing and the ability to perform quantitative analysis of mixtures. The potential to analyze degraded DNA by using NGS/MPS to sequence mitochondrial DNA and SNPs is discussed, as are the benefits and limitations of Rapid DNA instruments.

scholarly journals Progress in the implementation of massively parallel sequencing for forensic genetics: results of a European-wide survey among professional users

2021 ◽  
Author(s):  
Theresa E. Gross ◽  
Jan Fleckhaus ◽  
Peter M. Schneider
Keyword(s):  
Online Survey ◽  
Dna Analysis ◽  
Massively Parallel Sequencing ◽  
Forensic Genetics ◽  
Massively Parallel ◽  
Forensic Dna ◽  
Educational Training ◽  
Forensic Dna Analysis ◽  
Parallel Sequencing ◽  
Horizon 2020

AbstractA European-wide online survey was conducted to generate an overview on the state-of-the-art using massively parallel sequencing (MPS) platforms for forensic DNA analysis and DNA phenotyping among forensic practitioners in Europe. The survey was part of the dissemination activities of the “VISible Attributes through GEnomics – VISAGE” Horizon 2020 funded European research project [30], in preparation of a series of educational training activities. A total of 105 replies from 32 European countries representing participants from police, governmental, academic, and private laboratories providing professional services in the field of forensic genetics were included in the final analysis. Of these, 73% already own an MPS platform or are planning to acquire one within the next 1–2 years. One-third of the participants have already carried out MPS-based STR sequencing, identity, or ancestry SNP typing. A total of 23–40% of participants are planning to explore all FDP applications showing the overall very high interest in using MPS for the whole range of forensic MPS markers and applications. About 50% of the participants have previously gathered experience using forensic DNA phenotyping (FDP) markers based on conventional (i.e., not MPS-based) DNA typing methods. A total of 55% of the participants have attended training on the general use of MPS technology, but 36% have received no training whatsoever. Accordingly, 90% have expressed high or medium interest to attend training on the analysis and interpretation of DNA phenotyping data for predicting appearance, ancestry, and age. The results of our survey will provide valuable information for organizing relevant training workshops on all aspects of MPS-based DNA phenotyping for the forensic genetics scientific community.

scholarly journals FastID: Extremely Fast Forensic DNA Comparisons

2017 ◽  
Author(s):  
Darrell O. Ricke
Keyword(s):  
Massively Parallel Sequencing ◽  
Forensic Analysis ◽  
Massively Parallel ◽  
Forensic Dna ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Str Loci ◽  
Forensic Samples ◽  
Scalable Analysis ◽  
Short Tandem

AbstractRapid analysis of DNA forensic samples can have a critical impact on time sensitive investigations. Analysis of forensic DNA samples by massively parallel sequencing is creating the next gold standard for DNA forensic analysis. This technology enables the expansion of forensic profiles from the current 20 short tandem repeat (STR) loci to tens of thousands of single nucleotide polymorphism (SNP) loci. A forensic search scales by the product of the number of loci and the number of profile comparisons. This paper introduces a method (FastID) to address the need for rapid scalable analysis of DNA forensic samples (patent pending)[1]. FastID can search a profile of 2,500 SNP loci against 20 million profiles in 5.08 seconds using a single computational thread on a laptop (Intel i7 4.0 GHz).

scholarly journals Developments in forensic DNA analysis

2021 ◽  
Author(s):  
Penelope R. Haddrill
Keyword(s):  
Dna Analysis ◽  
Massively Parallel Sequencing ◽  
Forensic Genetics ◽  
Snp Markers ◽  
Tissue Type ◽  
Forensic Dna ◽  
Forensic Dna Analysis ◽  
Dna Database ◽  
Forensic Practice ◽  
Potential Benefits

The analysis of DNA from biological evidence recovered in the course of criminal investigations can provide very powerful evidence when a recovered profile matches one found on a DNA database or generated from a suspect. However, when no profile match is found, when the amount of DNA in a sample is too low, or the DNA too degraded to be analysed, traditional STR profiling may be of limited value. The rapidly expanding field of forensic genetics has introduced various novel methodologies that enable the analysis of challenging forensic samples, and that can generate intelligence about the donor of a biological sample. This article reviews some of the most important recent advances in the field, including the application of massively parallel sequencing to the analysis of STRs and other marker types, advancements in DNA mixture interpretation, particularly the use of probabilistic genotyping methods, the profiling of different RNA types for the identification of body fluids, the interrogation of SNP markers for predicting forensically relevant phenotypes, epigenetics and the analysis of DNA methylation to determine tissue type and estimate age, and the emerging field of forensic genetic genealogy. A key challenge will be for researchers to consider carefully how these innovations can be implemented into forensic practice to ensure their potential benefits are maximised.

Amelogenin test abnormalities revealed in Belarusian population during forensic DNA analysis

2015 ◽  
Vol 15 ◽  
pp. 98-104 ◽  
Author(s):  
Sergey Borovko ◽  
Alena Shyla ◽  
Victorya Korban ◽  
Alexandra Borovko
Keyword(s):  
Dna Analysis ◽  
Forensic Dna ◽  
Forensic Dna Analysis

scholarly journals Discovering a double murder through skeletal remains: A case report

2018 ◽  
Vol 59 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Samuele Manzoni ◽  
Andrea Ossoli ◽  
Venusia Cortellini ◽  
Andrea Verzeletti
Keyword(s):  
Dna Analysis ◽  
Skeletal Remains ◽  
Post Mortem ◽  
Forensic Dna ◽  
Biological Profile ◽  
Life Imprisonment ◽  
Forensic Dna Analysis ◽  
Complex Process ◽  
Forensic Case ◽  
Two Bodies

Forensic examination of human remains is a complex process that relies on the contribution of multidisciplinary forensic medicine specialties. Here we present a complex forensic case regarding a double murder whose victims were found almost completely skeletonized. Post-mortem investigations allowed us to define the biological profile of the two bodies (ancestry, sex, age and stature), to discover their identity through forensic DNA analysis, and to detect peri-mortem injuries caused by firearms and stabbing weapons. Three men were recognized as involved in the crime and two of them were condemned to life imprisonment for homicide. The judges accepted the reconstruction of the crime promoted by the Prosecutor (double firearm murder).

scholarly journals Improved forensic DNA analysis through the use of alternative DNA polymerases and statistical modeling of DNA profiles

BioTechniques ◽  
2009 ◽  
Vol 47 (5) ◽  
pp. 951-958 ◽  
Author(s):  
Johannes Hedman ◽  
Anders Nordgaard ◽  
Birgitta Rasmusson ◽  
Ricky Ansell ◽  
Peter Rådström
Keyword(s):  
Statistical Modeling ◽  
Dna Analysis ◽  
Dna Polymerases ◽  
Forensic Dna ◽  
Forensic Dna Analysis ◽  
Dna Profiles
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