Purification and Characterization of the Neutral Endopeptidase from Human Kidney

1983 ◽  
Vol 94 (1) ◽  
pp. 17-24 ◽  
Author(s):  
Masatoshi ISHIDA ◽  
Michio OGAWA ◽  
Goro KOSAKI ◽  
Tomohjro MEGA ◽  
Tokuji IKENAKA
Keyword(s):  
Human Kidney ◽  
Neutral Endopeptidase ◽  
Purification And Characterization

scholarly journals Further purification and characterization of the acid α-glucosidase

1968 ◽  
Vol 108 (2) ◽  
pp. 161-167 ◽  
Author(s):  
F. Auricchio ◽  
C. B. Bruni ◽  
V. Sica
Keyword(s):  
Rat Liver ◽  
Gel Electrophoresis ◽  
Gel Filtration ◽  
Human Kidney ◽  
Purification And Characterization ◽  
Preliminary Purification ◽  
Kidney Enzyme ◽  
Michaelis Constants ◽  
Final Degree

1. Centrifugation of rat liver acid glucosidase, which had been purified by adsorption on dextran gel, on a density gradient of sucrose showed the enzyme to be impure. 2. Preliminary purification of the enzyme before the gel filtration improved the final degree of purity of this preparation. Disc gel electrophoresis of this preparation showed a single band of protein. 3. The sedimentation co-efficient and the molecular weight determined on a sucrose gradient were 4·9–5·1s and 76000–83000 respectively for the rat liver enzyme, and 5·6s and 97000 for the acid α-glucosidase purified by means of the same procedure from the human kidney. 4. The Michaelis constants of rat liver and human kidney enzyme were 4·7×10−3m and 13·6×10−3m respectively with maltose as substrate. 5. The enzyme from both tissues was inhibited by tris and by erythritol. The inhibition of the rat liver acid glucosidase by erythritol was competitive.

The purification and characterization of human kidney l-arginine:Glycine amidinotransferase

1986 ◽  
Vol 251 (2) ◽  
pp. 747-755 ◽  
Author(s):  
Myron D. Gross ◽  
Mark A. Eggen ◽  
Alexander M. Simon ◽  
John F. Van Pilsum
Keyword(s):  
Human Kidney ◽  
Purification And Characterization

Purification and characterization of human kidney plasminogen activator dissimilar to urokinase

1982 ◽  
Vol 717 (2) ◽  
pp. 327-336 ◽  
Author(s):  
Katsuo Sueishi ◽  
Shigeru Nanno ◽  
Takashi Okamura ◽  
Sadami Inoue ◽  
Kenzo Tanaka
Keyword(s):  
Plasminogen Activator ◽  
Human Kidney ◽  
Purification And Characterization

Purification and characterization of recombinant human cathepsin E expressed in human kidney cell line 293

2004 ◽  
Vol 37 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Maria G Cappiello ◽  
Zhongren Wu ◽  
Boyd B Scott ◽  
Gerard M McGeehan ◽  
Richard K Harrison
Keyword(s):  
Cell Line ◽  
Kidney Cell ◽  
Human Kidney ◽  
Kidney Cell Line ◽  
Purification And Characterization ◽  
Cathepsin E ◽  
Human Cathepsin

Purification and characterization of glutathione S-transferases in human kidney

1987 ◽  
Vol 166 (2-3) ◽  
pp. 207-218 ◽  
Author(s):  
Noboru Tateoka ◽  
Shigeki Tsuchida ◽  
Yasushi Soma ◽  
Kiyomi Sato
Keyword(s):  
Human Kidney ◽  
Purification And Characterization ◽  
Glutathione S Transferases

Purification and characterization of a membrane-bound neutral endopeptidase from human placenta

1987 ◽  
Vol 37 (1) ◽  
pp. 22-30 ◽  
Author(s):  
Yasuhiro Watanabe ◽  
Yuka Kumagai ◽  
Yoshimitsu Shimamori ◽  
Yukio Fujimoto
Keyword(s):  
Human Placenta ◽  
Neutral Endopeptidase ◽  
Purification And Characterization ◽  
Membrane Bound

Purification and characterization of a neutral endopeptidase-like enzyme from human urine

1998 ◽  
Vol 16 (Supplement) ◽  
pp. 1971-1978 ◽  
Author(s):  
Giovana S. Di Marco ◽  
Beata M.R. Quinto ◽  
Maria Juliano ◽  
Adriana K. Carmona ◽  
Regina C.R. Stella ◽  
...  
Keyword(s):  
Human Urine ◽  
Neutral Endopeptidase ◽  
Purification And Characterization
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