Purification and Characterization of Proteinase Inhibitors from Winged Bean (Psophocarpus tetragonolobus (L.) DC.) Seeds1

1986 ◽  
Vol 99 (4) ◽  
pp. 1147-1155 ◽  
Author(s):  
Hiroshi SHIBATA ◽  
Saburo HARA ◽  
Tokuji IKENAKA ◽  
Jiro ABE
Keyword(s):  
Proteinase Inhibitors ◽  
Winged Bean ◽  
Psophocarpus Tetragonolobus ◽  
Purification And Characterization

Purification and Characterization of the Acidic Lectin from Winged Bean Seeds

1986 ◽  
Vol 50 (7) ◽  
pp. 1847-1853
Author(s):  
Masako Higuchi ◽  
Yutaka Ohtani ◽  
Kazuo Iwai
Keyword(s):  
Winged Bean ◽  
Purification And Characterization

scholarly journals Proteases of germinating winged-bean (Psophocarpus tetragonolobus) seeds: purification and characterization of an acidic protease

1996 ◽  
Vol 313 (2) ◽  
pp. 423-429 ◽  
Author(s):  
Rajamma USHA ◽  
Manoranjan SINGH
Keyword(s):  
Physiological Role ◽  
Immunoblot Analysis ◽  
Protein Band ◽  
Winged Bean ◽  
Psophocarpus Tetragonolobus ◽  
Ph Optimum ◽  
Apparent Homogeneity ◽  
Sds Page ◽  
Storage Protein Mobilization

Two major classes of protease are shown to occur in germinating winged-bean (Psophocarpus tetragonolobus) seeds, by assaying extracts at pH 8.0 and pH 5.1 with [14C]gelatin as substrate. At pH 8.0, the activity profile of the enzyme shows a steady rise throughout the period of germination, whereas the activity at the acidic pH is very low up to day 5 and then increases sharply reaching a peak on day 11, followed by an equally sharp decline. The winged-bean acidic protease (WbAP) has been purified to apparent homogeneity, as attested by a single protein band on both PAGE and SDS/PAGE. WbAP is a monomeric enzyme with a molecular mass of 35 kDa and a pH optimum of 6.0. It is a thiol protease that does not belong to the papain family and it has tightly bound Ca2+ as shown by 45Ca2+-exchange studies. Besides gelatin and casein, it hydrolyses a 29 kDa winged-bean protein, indicating a prospective physiological role for it in storage-protein mobilization. Immunoblot analysis shows that it occurs only in the seeds and sprouting tubers of this plant and also that it is synthesized in developing seeds just before desiccation. It appears that the newly synthesized enzyme is inactive, and activation takes place around day 6 of germination. However, neither the mechanism of activation nor the signal that triggers it is clearly understood.

scholarly journals Characterization of winged bean (<i>Psophocarpus tetragonolobus</i> (L.) DC.) based on molecular, chemical and physiological parameters

2013 ◽  
Vol 03 (04) ◽  
pp. 187-197 ◽  
Author(s):  
Chandra Sekhar Mohanty ◽  
Sushma Verma ◽  
Vinayak Singh ◽  
Shahina Khan ◽  
Priyanka Gaur ◽  
...  
Keyword(s):  
Physiological Parameters ◽  
Winged Bean ◽  
Psophocarpus Tetragonolobus
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