Characterization of PmHHXM, a New Broad-spectrum Powdery Mildew Resistance Gene in Chinese Wheat Landrace Honghuaxiaomai

Plant Disease ◽  
2021 ◽  
Author(s):  
Shulin Xue ◽  
Mingxue Lu ◽  
Shanshan Hu ◽  
Hongxing Xu ◽  
Yuyu Ma ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Broad Spectrum ◽  
Powdery Mildew Resistance ◽  
Dominant Gene ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Wheat Landrace ◽  
Chinese Wheat

Powdery mildew, caused by fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is one of agronomically important and widespread wheat diseases causing severe yield losses. Deployment of broad‐spectrum disease-resistance genes is the preferred strategy to prevent this pathogen. Chinese wheat landrace Honghuaxiaomai (HHXM) was resistant to all 23 tested Bgt isolates at the seedling stage. The F1, F2, and F2:3 progenies derived from the cross HHXM × Yangmai 158 were used in this study, and genetic analysis revealed that a single dominant gene, designated as PmHHXM, conferred resistance to Bgt isolate E09. Bulked segregant analysis and molecular mapping initially located PmHHXM to the distal region of chromosome 4AL. To fine map PmHHXM, two critical recombinants were identified from 592 F2 plants and delimited PmHHXM to a 0.18-cM Xkasp475200–Xhnu552 interval covering 1.77-Mb, in which a number of disease resistance-related gene clusters were annotated. Comparative mapping of this interval revealed a perturbed synteny among Triticeae species. This study reports the new powdery mildew resistance gene PmHHXM that seems different from three known QTL/genes identified on chromosome 4AL and has significant values for further genetic improvement. Analysis of the polymorphisms of 13 co-segregating markers between HHXM and 170 modern wheat cultivars indicates that Xhnu227 and Xsts478700 developed here are ideal for marker-assisted introgression of this resistance gene in wheat breeding.

Characterization of Pm59, a novel powdery mildew resistance gene in Afghanistan wheat landrace PI 181356

2018 ◽  
Vol 131 (5) ◽  
pp. 1145-1152 ◽  
Author(s):  
Chengcheng Tan ◽  
Genqiao Li ◽  
Christina Cowger ◽  
Brett F. Carver ◽  
Xiangyang Xu
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Wheat Landrace

Genetic map of the powdery mildew resistance gene in soybean PI 243540

Genome ◽  
2010 ◽  
Vol 53 (5) ◽  
pp. 400-405 ◽  
Author(s):  
Sung-Taeg Kang ◽  
M.A. Rouf Mian
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Dominant Gene ◽  
Common Disease ◽  
Single Dominant Gene ◽  
Powdery Mildew Resistance Gene ◽  
Chromosome 16 ◽  
Mildew Resistance

Powdery mildew (caused by Microsphaera diffusa Cooke & Peck) is a common disease of soybean in many soybean-growing regions of the world and under greenhouse conditions. The previously reported Rmd locus of soybean for resistance to powdery mildew was mapped on soybean molecular linkage group J (chromosome 16). We have discovered a single dominant gene in PI 243540 that provides season-long resistance to powdery mildew. The objective of this study was to map the powdery mildew resistance gene in PI 243540 with PCR-based molecular markers. One hundred eighty-four F2 plants and their F2:3 families from a cross between the powdery mildew susceptible cultivar ‘Wyandot’ and PI 243540 were screened with M. diffusa in greenhouses. Bulked segregant analysis (BSA) with SSR markers was used to identify the tentative genomic location of the gene. The BSA localized the gene to a genomic region in soybean chromosome 16. A linkage map with seven SSR and six SNP markers flanking the gene was constructed. We positioned the gene between SSR marker Sat_224 and SNP marker BARC-021875-04228 at distances of 9.6 and 1.3 cM from the markers, respectively. The map position of the gene was slightly different from previously reported map positions of the only known Rmd locus. We have mapped a single dominant gene, tentatively called Rmd_PI243540, near the previously known Rmd locus on chromosome 16. The molecular markers flanking the gene will be useful for marker-assisted selection of this gene.

scholarly journals Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing

2019 ◽  
Author(s):  
Huanhuan Li ◽  
Zhenjie Dong ◽  
Chao Ma ◽  
Xiubin Tian ◽  
Zhiguo Xiang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Disease Resistance Gene ◽  
R Genes ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Resistance Gene Candidates

AbstractPowdery mildew is one of the most widespread diseases of wheat. Breeding resistant varieties by utilization of resistance genes is considered as the most economic and effective method of controlling this disease. Previous study showed that the gene(s) at 2Mb in Chinese Spring (CS)-Aegilops biuncialis 2Mb disomic addition line TA7733 conferred high resistance to powdery mildew. In this study, 15 Bgt isolates prevalent in different regions of China were used to further test the resistance spectrum of TA7733. As a result, TA7733 was high resistance to all tested isolates, indicating that the gene(s) on chromosome 2Mb was broad-spectrum powdery mildew resistance. In order to mine resistance gene candidates and develop 2Mb-specific molecular markers to assist the transfer resistance gene(s) at chromosome 2Mb, RNA-seq of TA7733 and CS was conducted before and after Bgt-infection, generating a total of 158,953 unigenes. Of which, 7,278 unigenes were TA7733-specific which were not expressed in CS, and 295 out of these 7,278 unigenes were annotated as R genes. Based on Blastn against with CS Ref Seq v1.0, 61 R genes were further mapped to homoeologous group 2. Analysis of R gene-specific molecular markers designed from R gene sequences verified 40 out of 61 R genes to be 2Mb specific. Annotation of these 40 R genes showed most genes encoded nucleotide binding leucine rich repeat (NLR) protein, being most likely resistance gene candidates. The broad-spectrum powdery mildew resistance gene(s), disease resistance gene candidates, and functional molecular markers of 2Mb-specific in present study will not only lay foundations for transferring disease resistance gene(s) from 2Mb to common wheat by inducing CS-Ae. biuncialis homoeologous recombination, but also provide useful candidates for isolating and cloning resistance gene(s) and dissecting molecular and genetic mechanisms of disease resistance from 2Mb.

scholarly journals Fine Mapping a Broad-Spectrum Powdery Mildew Resistance Gene in Chinese Landrace Datoumai, PmDTM, and Its Relationship with Pm24

Plant Disease ◽  
2020 ◽  
Vol 104 (6) ◽  
pp. 1709-1714
Author(s):  
Nan Lu ◽  
Mingxue Lu ◽  
Pan Liu ◽  
Hongxing Xu ◽  
Xiaolong Qiu ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Dominant Gene ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Resistant Varieties ◽  
Chinese Landrace ◽  
Chinese Wheat ◽  
Simple Sequence

Powdery mildew, caused by the biotrophic fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is a globally important wheat disease causing severe yield losses, and deployment of resistant varieties is the preferred choice for managing this disease. Chinese wheat landrace Datoumai was resistant to 22 of 23 Bgt isolates at the seedling stage. Genetic analysis based on the inoculation of Bgt isolate E09 on the F1, F2, and F2:3 populations derived from the cross Datoumai × Huixianhong revealed that the powdery mildew resistance of Datoumai is controlled by a single dominant gene, temporarily designated as PmDTM. Bulked segregant analysis and simple sequence repeat mapping with 200 F2 plants showed that PmDTM was located in the same genetic region as Pm24 on chromosome 1DS. To fine map PmDTM, 12 critical recombinants were identified from 1,192 F2 plants and delimited PmDTM to a 0.5-cM Xhnu58800 to Xhnu59000 interval covering 180.5 Kb (38,728,125 to 38,908,656 bp) on chromosome 1DS, and only one highly confident gene, TraesCS1D02G058900, was annotated within this region. TraesCS1D02G058900 encodes a receptor-like serine/threonine-protein kinase (STK), and a 6-bp deletion in exon 5 may confer the resistance to powdery mildew. Allele frequency analysis indicated that the STK allele with 6-bp deletion was only present in three landraces (Datoumai, Chiyacao [Pm24], and Hulutou) and was absent in all of the 353 Chinese modern cultivars and 147 foreign cultivars. These results demonstrate that PmDTM is mapped to the same locus as Pm24 and can be widely used to enhance powdery mildew resistance in wheat growing regions worldwide.

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