scholarly journals Discovery of powdery mildew resistance gene candidates from Aegilops biuncialis chromosome 2Mb based on transcriptome sequencing

2019 ◽  
Author(s):  
Huanhuan Li ◽  
Zhenjie Dong ◽  
Chao Ma ◽  
Xiubin Tian ◽  
Zhiguo Xiang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Disease Resistance Gene ◽  
R Genes ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Resistance Gene Candidates

AbstractPowdery mildew is one of the most widespread diseases of wheat. Breeding resistant varieties by utilization of resistance genes is considered as the most economic and effective method of controlling this disease. Previous study showed that the gene(s) at 2Mb in Chinese Spring (CS)-Aegilops biuncialis 2Mb disomic addition line TA7733 conferred high resistance to powdery mildew. In this study, 15 Bgt isolates prevalent in different regions of China were used to further test the resistance spectrum of TA7733. As a result, TA7733 was high resistance to all tested isolates, indicating that the gene(s) on chromosome 2Mb was broad-spectrum powdery mildew resistance. In order to mine resistance gene candidates and develop 2Mb-specific molecular markers to assist the transfer resistance gene(s) at chromosome 2Mb, RNA-seq of TA7733 and CS was conducted before and after Bgt-infection, generating a total of 158,953 unigenes. Of which, 7,278 unigenes were TA7733-specific which were not expressed in CS, and 295 out of these 7,278 unigenes were annotated as R genes. Based on Blastn against with CS Ref Seq v1.0, 61 R genes were further mapped to homoeologous group 2. Analysis of R gene-specific molecular markers designed from R gene sequences verified 40 out of 61 R genes to be 2Mb specific. Annotation of these 40 R genes showed most genes encoded nucleotide binding leucine rich repeat (NLR) protein, being most likely resistance gene candidates. The broad-spectrum powdery mildew resistance gene(s), disease resistance gene candidates, and functional molecular markers of 2Mb-specific in present study will not only lay foundations for transferring disease resistance gene(s) from 2Mb to common wheat by inducing CS-Ae. biuncialis homoeologous recombination, but also provide useful candidates for isolating and cloning resistance gene(s) and dissecting molecular and genetic mechanisms of disease resistance from 2Mb.

Genetic map of the powdery mildew resistance gene in soybean PI 243540

Genome ◽  
2010 ◽  
Vol 53 (5) ◽  
pp. 400-405 ◽  
Author(s):  
Sung-Taeg Kang ◽  
M.A. Rouf Mian
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Dominant Gene ◽  
Common Disease ◽  
Single Dominant Gene ◽  
Powdery Mildew Resistance Gene ◽  
Chromosome 16 ◽  
Mildew Resistance

Powdery mildew (caused by Microsphaera diffusa Cooke & Peck) is a common disease of soybean in many soybean-growing regions of the world and under greenhouse conditions. The previously reported Rmd locus of soybean for resistance to powdery mildew was mapped on soybean molecular linkage group J (chromosome 16). We have discovered a single dominant gene in PI 243540 that provides season-long resistance to powdery mildew. The objective of this study was to map the powdery mildew resistance gene in PI 243540 with PCR-based molecular markers. One hundred eighty-four F2 plants and their F2:3 families from a cross between the powdery mildew susceptible cultivar ‘Wyandot’ and PI 243540 were screened with M. diffusa in greenhouses. Bulked segregant analysis (BSA) with SSR markers was used to identify the tentative genomic location of the gene. The BSA localized the gene to a genomic region in soybean chromosome 16. A linkage map with seven SSR and six SNP markers flanking the gene was constructed. We positioned the gene between SSR marker Sat_224 and SNP marker BARC-021875-04228 at distances of 9.6 and 1.3 cM from the markers, respectively. The map position of the gene was slightly different from previously reported map positions of the only known Rmd locus. We have mapped a single dominant gene, tentatively called Rmd_PI243540, near the previously known Rmd locus on chromosome 16. The molecular markers flanking the gene will be useful for marker-assisted selection of this gene.

Characterization of PmHHXM, a New Broad-spectrum Powdery Mildew Resistance Gene in Chinese Wheat Landrace Honghuaxiaomai

Plant Disease ◽  
2021 ◽  
Author(s):  
Shulin Xue ◽  
Mingxue Lu ◽  
Shanshan Hu ◽  
Hongxing Xu ◽  
Yuyu Ma ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Powdery Mildew ◽  
Resistance Gene ◽  
Broad Spectrum ◽  
Powdery Mildew Resistance ◽  
Dominant Gene ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Wheat Landrace ◽  
Chinese Wheat

Powdery mildew, caused by fungal pathogen Blumeria graminis f. sp. tritici (Bgt), is one of agronomically important and widespread wheat diseases causing severe yield losses. Deployment of broad‐spectrum disease-resistance genes is the preferred strategy to prevent this pathogen. Chinese wheat landrace Honghuaxiaomai (HHXM) was resistant to all 23 tested Bgt isolates at the seedling stage. The F1, F2, and F2:3 progenies derived from the cross HHXM × Yangmai 158 were used in this study, and genetic analysis revealed that a single dominant gene, designated as PmHHXM, conferred resistance to Bgt isolate E09. Bulked segregant analysis and molecular mapping initially located PmHHXM to the distal region of chromosome 4AL. To fine map PmHHXM, two critical recombinants were identified from 592 F2 plants and delimited PmHHXM to a 0.18-cM Xkasp475200–Xhnu552 interval covering 1.77-Mb, in which a number of disease resistance-related gene clusters were annotated. Comparative mapping of this interval revealed a perturbed synteny among Triticeae species. This study reports the new powdery mildew resistance gene PmHHXM that seems different from three known QTL/genes identified on chromosome 4AL and has significant values for further genetic improvement. Analysis of the polymorphisms of 13 co-segregating markers between HHXM and 170 modern wheat cultivars indicates that Xhnu227 and Xsts478700 developed here are ideal for marker-assisted introgression of this resistance gene in wheat breeding.

scholarly journals Physical Mapping of Pm57, a Powdery Mildew Resistance Gene Derived from Aegilops searsii

2019 ◽  
Author(s):  
Zhenjie Dong ◽  
Xiubin Tian ◽  
Chao Ma ◽  
Qing Xia ◽  
Beilin Wang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Binding Site ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Powdery Mildew Resistance ◽  
Nucleotide Binding ◽  
Leucine Rich Repeat ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of many severe diseases that threaten bread wheat (Triticum aestivum L.) yield and quality worldwide. The discovery and deployment of powdery mildew resistance genes (Pm) can prevent this disease epidemic in wheat. In a previous study, we transferred the powdery mildew resistance gene Pm57 from Aegilops searsii into common wheat and cytogenetically mapped the gene in a chromosome region with the fraction length (FL) 0.75-0.87, which represents 12% of 2Ss#1 segment on the long arm of chromosome 2Ss#1. In this study, we performed RNA-Seq on three infected and mock-infected wheat-Ae. searsii 2Ss#1 introgression lines with Bgt-isolates inoculation at 0, 12, 24, and 48 hours after inoculation. Then we designed 79 molecular markers based on transcriptome sequences and physically mapped them to Ae. searsii chromosome 2Ss#1- in seven intervals. We used these markers to identify 46 wheat-Ae. searsii 2Ss#1 recombinants induced by ph1b, a deletion mutant of pairing homoelogous (Ph) genes. Analysis of the 46 ph1b-induced 2Ss#1L recombinants with different Bgt-responses using 28 2Ss#1L-specific molecular markers in the interval FL0.72-0.87 where Pm57 is located, and the flanking intervals, we physically mapped Pm57 gene on the long arm of 2Ss#1 in a 5.13 Mb genomic region, which was flanked by markers X67593 (773.72 Mb) and X62492 (778.85 Mb). By comparative synteny analysis of the corresponding region on chromosome 2B in Chinese spring (T. aestivum L.) with other model species we identified ten genes that are putative plant defense-related (R) genes which includes six coiled-coil nucleotide-binding site-leucine-rich repeat (CNL), three nucleotide-binding site-leucine-rich repeat (NL) and a leucine-rich receptor-like repeat (RLP) encoding proteins. This study will lay a foundation for further cloning of Pm57, and benefit the understanding of interactions between resistance genes of wheat and powdery mildew pathogens.

scholarly journals Fine mapping and identification of candidate genes for the peach powdery mildew resistance gene Vr3

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Neus Marimon ◽  
Jordi Luque ◽  
Pere Arús ◽  
Iban Eduardo
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Candidate Genes ◽  
Resistance Gene ◽  
Prunus Persica ◽  
Powdery Mildew Resistance ◽  
Disruptive Effect ◽  
Powdery Mildew Resistance Gene ◽  
Positional Candidate ◽  
Mildew Resistance

Abstract Powdery mildew is one of the major diseases of peach (Prunus persica), caused by the ascomycete Podosphaera pannosa. Currently, it is controlled through calendar-based fungicide treatments starting at petal fall, but an alternative is to develop resistant peach varieties. Previous studies mapped a resistance gene (Vr3) in interspecific populations between almond (‘Texas’) and peach (‘Earlygold’). To obtain molecular markers highly linked to Vr3 and to reduce the number of candidate genes, we fine-mapped Vr3 to a genomic region of 270 kb with 27 annotated genes. To find evidence supporting one of these positional candidate genes as being responsible of Vr3, we analyzed the polymorphisms of the resequences of both parents and used near-isogenic lines (NILs) for expression analysis of the positional candidate genes in symptomatic or asymptomatic leaves. Genes differentially expressed between resistant and susceptible individuals were annotated as a Disease Resistance Protein RGA2 (Prupe2G111700) or an Eceriferum 1 protein involved in epicuticular wax biosynthesis (Prupe2G112800). Only Prupe2G111700 contained a variant predicted to have a disruptive effect on the encoded protein, and was overexpressed in both heterozygous and homozygous individuals containing the Vr3 almond allele, compared with susceptible individuals. This information was also useful to identify and validate molecular markers tightly linked and flanking Vr3. In addition, the NILs used in this work will facilitate the introgression of this gene into peach elite materials, alone or pyramided with other known resistance genes such as peach powdery mildew resistance gene Vr2.

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