scholarly journals Impatiens necrotic spot virus and Tomato spotted wilt virus Diagnosed in Phalaenopsis Orchids from Two Florida Nurseries

Plant Disease ◽  
2007 ◽  
Vol 91 (11) ◽  
pp. 1515-1515 ◽  
Author(s):  
C. A. Baker ◽  
D. Davison ◽  
L. Jones
Keyword(s):  
Nucleocapsid Protein ◽  
Tomato Spotted Wilt Virus ◽  
Protein Gene ◽  
Impatiens Necrotic Spot Virus ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Tomato Spotted Wilt ◽  
Local Lesion Host ◽  
Nucleocapsid Protein Gene ◽  
Wilt Virus

In October 2006 (Arcadia, FL) and January 2007 (Sorrento, FL), several white Phalaenopsis orchids with large chlorotic/necrotic ringspot symptoms were sent to the Division of Plant Industry, Gainesville, FL. Symptomatic leaf tissues were tested with the Agdia immunostick-comb (Agdia, Elkhart, IN) for Impatiens necrotic spot virus (INSV), Tomato spotted wilt virus (TSWV), Cucumber mosaic, and Tobacco mosaic virus. Plants from the nursery in Sorrento, FL tested positive for TSWV, while those from the nursery in Arcadia, FL tested positive for INSV. Symptomless leaves from the infected plants tested negative for the viruses with the immunostick-comb. The plants also were tested for TSWV and INSV by double-antibody (DAS)-ELISA (Agdia Inc.) with the same results. Total RNA was extracted from one symptomatic orchid leaf from each nursery. Reverse transcription (RT)-PCR was performed with the universal tospovirus primer set BR60and BR65 (1). PCR bands of the expected size were amplified from each leaf. PCR products were sequenced directly. The orchid leaf that tested positive for TSWV by ELISA produced a 495-bp sequence with 97% identity to several isolates of the TSWV nucleocapsid protein gene listed in GenBank (Accession Nos. AY744479, AY8770391, DQ376185, and AF02659). The orchid leaf that tested positive for INSV by ELISA produced a 396-bp sequence with 98 to 99% identity to several isolates of the INSV nucleocapsid protein gene (Accession Nos. D00914, DQ425096, X66972, and AD109100). Although these viruses have been reported a few times in orchids previously (2,3), to our knowledge, this is the first time they have been reported in this host in Florida. In addition, white Phalaenopsis spp. appears to be a local lesion host and not a systemic host for these viruses. References: (1) M. Eiras et al. Fitopatol. Bras. 26:170, 2001. (2) J. S. Hu et al. Plant Dis. 77:464, 1993. (3) S. T. Koike and D. E. Mayhew. Orchids 70:746, 2001.

First Report on the Incidence of Mixed Infections of Impatiens necrotic spot virus (INSV) and Tomato spotted wilt virus (TSWV) in Tobacco Grown in Georgia, South Carolina, and Virginia

2003 ◽  
Vol 4 (1) ◽  
pp. 40 ◽  
Author(s):  
N. Martínez-Ochoa ◽  
A. S. Csinos ◽  
E. B. Whitty ◽  
A. W. Johnson ◽  
M. J. Parrish
Keyword(s):  
South Carolina ◽  
Tomato Spotted Wilt Virus ◽  
Impatiens Necrotic Spot Virus ◽  
Mixed Infections ◽  
Necrotic Spot ◽  
Spot Virus ◽  
Greenhouse Production ◽  
Tomato Spotted Wilt ◽  
Serious Disease ◽  
Wilt Virus

Tomato spotted wilt caused by Tomato spotted wilt virus (TSWV) continues to be a serious disease problem on tobacco (Nicotiana tabacum L.), peanut (Arachis hypogaea L.), tomato (Lycopersicon esculentum Mill.), and pepper (Capsicum annum L.) in the southeastern United States. Impatiens necrotic spot virus (INSV, formerly known as TSWV-I) is an emerging virus found mostly in greenhouse production of ornamentals and is also vectored by thrips. A few years ago INSV was detected in peanut in Georgia and Texas and its occurrence appears to be increasing). Mixed infections of TSWV and INSV in tobacco have been observed within the last two years in North Carolina and Kentucky. Our objective was to sample several locations in Georgia, Florida, South Carolina and Virginia to confirm and report the presence of natural TSWV and INSV mixed infections in tobacco. Accepted for publication 14 March 2003. Published 17 April 2003.

scholarly journals Occurrence of Impatiens necrotic spot virus and Tomato spotted wilt virus on Potatoes in Iran

Plant Disease ◽  
2012 ◽  
Vol 96 (5) ◽  
pp. 771-771 ◽  
Author(s):  
R. Pourrahim ◽  
A. R. Golnaraghi ◽  
Sh. Farzadfar
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Natural Infection ◽  
Impatiens Necrotic Spot Virus ◽  
Necrotic Spot ◽  
Leaf Sample ◽  
Potato Leaf ◽  
Spot Virus ◽  
Tomato Spotted Wilt ◽  
Necrotic Spots ◽  
Wilt Virus

There have been an increasing number of records of the natural infection of various crops and ornamentals in Iran with Impatiens necrotic spot virus (INSV), Tomato spotted wilt virus (TSWV), and Tomato yellow fruit ring virus (TYFRV), a recently proposed species in the genus Topovirus (3). TYFRV, originally believed to be TSWV and named as such, has been previously reported to occur in Iranian potato fields (2). During the growing seasons of 2004 to 2006, surveys were conducted in potato fields in different potato-producing (Solanum tuberosum) provinces of Iran (Ardabil, Azarbayejan-e-sharqi, Chaharmahal-va-bakhtiyari, Esfahan, Hamedan, Kerman, Khorasan, Khuzestan, Lorestan, Tehran, Qazvin, and Zanjan) to detect the presence of Tospovirus spp. infecting this crop, including Groundnut ringspot virus (GRSV), INSV, Iris yellow spot virus (IYSV), Tomato chlorotic spot virus (TCSV), TSWV, TYFRV, and Watermelon silver mottle virus (WSMoV). Overall, 186 fields were surveyed, and 2,823 potato leaf samples from plants showing tospovirus-like symptoms of chlorotic or necrotic spots, chlorosis, and necrosis were collected before or through the flowering stage, approximately 50 to 90 days after planting. Each leaf sample was tested by double-antibody sandwich (DAS)-ELISA using specific antisera (Bioreba, Reinach, Switzerland; Loewe, Sauerlach, Germany; DSMZ, Braunschweig, Germany) for the presence of the aforementioned tospoviruses. TYFRV, TSWV, and INSV were found in 24.0, 4.1, and 0.4% of the samples collected from 133, 51, and 7 fields surveyed, respectively. None of the samples had a positive reaction in ELISA to GRSV, IYSV, TCSV, and WSMoV. To confirm this testing, a number of the leaf samples that were found to be positive for INSV, TSWV, and TYFRV in ELISA tests were mechanically inoculated on Petunia × hybrid and Nicotiana benthamiana; the inoculated plants showed typical necrotic local lesions of tospoviruses and chlorotic or necrotic spots followed by systemic infection, respectively; their infection was subsequently confirmed by ELISA. The samples also were tested by reverse transcription-PCR technique using previously described specific primers (1,4). The PCR reaction resulted in the specific amplification of a 0.59-, 0.71-, and 0.67-kb (or 1.2-kb) fragment of INSV, TSWV, and TYFRV RNAs, respectively. This study showed that tospoviruses, especially TYFRV, are widespread in Iranian potato fields. It is hoped that the results may help us to improve a seed potato certification program in the future. To our knowledge, this is the first report of INSV and TSWV from potatoes in Iran. References: (1) A. R. Golnaraghi et al. Plant Dis. 92:1280, 2008. (2) R. Pourrahim et al. Plant Dis. 84:442, 2001. (3) S. Winter et al. Plant Pathol. 55:287, 2006. (4) H. Uga and S. Tsuda. Phytopathology 95:166, 2005.

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