scholarly journals First Report of Sclerotium rolfsii on Island Ash Seedlings in Taiwan

Plant Disease ◽  
2003 ◽  
Vol 87 (12) ◽  
pp. 1539-1539
Author(s):  
C. H. Fu ◽  
H. J. Hsieh ◽  
J. C. Yao
Keyword(s):  
Plant Protection ◽  
Sclerotium Rolfsii ◽  
Plant Diseases ◽  
Peat Moss ◽  
First Report ◽  
Republic Of China ◽  
Initial Symptoms ◽  
Stem Necrosis ◽  
The Republic Of China ◽  
The Republic

Island ash (Fraxinus formosana Hay.) is a large, semideciduous tree in Taiwan. It is used for forestation, a shade tree, and producing wood for furniture. During the summer of 2001, a sudden wilt of 1-year-old plants was observed in a nursery in northern Taiwan. Initial symptoms included stem necrosis at the soil line and yellowing and tan discoloration of the leaves. As stem necrosis progressed, infected plants wilted and died. Necrotic tissues were covered with white mycelium that differentiated into reddish brown, spherical (1 to 2 mm in diameter) sclerotia. Sclerotium rolfsii was consistently recovered from the surface of symptomatic stem sections that were disinfected for 1 min in 0.5% NaOCl and then plated on potato dextrose agar (PDA) amended with 100 ppm of ampicillin. Pathogenicity of two S. rolfsii isolates was confirmed on 1-year-old island ash seedlings grown in 12.7 cm- (5-in) plastic pots in a sterilized mixture of peat moss and vermiculite (3:1). Seedlings were inoculated with mycelia and sclerotia of the pathogen grown on PDA. Three plants each were inoculated with four disks (5 mm) of agar with mycelium and three were inoculated with 10 sclerotia that were placed on the soil surface around the base of each plant. Noninoculated plants served as controls. All plants were kept in a growth chamber at 25 to 35°C and >95% relative humidity. The test was repeated once. All inoculated plants developed symptoms within 14 days, while control plants remained symptomless. Sclerotia developed on infected tissues, and S. rolfsii was reisolated from symptomatic tissues. This disease has been observed on many species of plants (1), but to our knowledge, this is the first report of Southern blight of Island ash seedlings caused by S. rolfsii in Taiwan. Reference: (1) Y. P. Tsai ed. List of Plant Diseases in Taiwan. The Plant Protection Society of the Republic of China and The Phytopathological Society of the Republic of China, 1991.

scholarly journals First Report of Southern Blight of Silvery Messerschmidia Seedlings in Taiwan

Plant Disease ◽  
2007 ◽  
Vol 91 (9) ◽  
pp. 1198-1198
Author(s):  
C. Y. Chen ◽  
C. H. Fu ◽  
W. W. Hsiao ◽  
E. J. Sun
Keyword(s):  
Plant Protection ◽  
Plant Diseases ◽  
First Report ◽  
Southern Blight ◽  
Republic Of China ◽  
Agar Plug ◽  
Initial Symptoms ◽  
Stem Necrosis ◽  
The Republic Of China ◽  
The Republic

Silvery messerschmidia, Messerschmidia argentea (L.) Johnston, of the Boraginaceae, is indigenous to Taiwan and grown as an ornamental, for windbreaks, or as a shade tree. During the summer of 2005, a sudden wilt of 1-year-old plants was observed in a nursery in central Taiwan. Initial symptoms included stem necrosis at the collar, leaf yellowing, and tan discoloration of leaves. As stem necrosis progressed, infected plants wilted, defoliated, and died. Necrotic tissues were covered with whitish mycelium with clamp connections that formed reddish brown spherical (1 to 2.2 mm in diameter) sclerotia. A fungus was consistently recovered from the interface of diseased and healthy stem tissue, disinfested for 1 min in 0.5% NaOCl, and plated on Difco (Sparks, MD) potato dextrose agar (PDA) amended with 100 ppm of ampicillin. Pure cultures were prepared by transferring single hyphal tips to PDA, and Sclerotium rolfsii (Sacc.) was identified (1). Pathogenicity of two S. rolfsii isolates was confirmed by inoculating 3-month-old silvery messerschmidia seedlings grown in pots. Inoculum consisted of a single agar disk of a 7-day-old culture used per pot or a single sclerotium produced in 10 days on PDA and added per pot. Both the mycelium on the 0.5-cm-diameter agar plug and the sclerotium touched the base of the plant stem. Four plants were inoculated with mycelia, four with sclerotia, and four were noninoculated controls. All plants were kept in a growth chamber at 25 to 35°C with relative humidity of more than 95%. Initially, the basal stems were covered by whitish mycelia growth with a fanlike pattern from the inoculum, and brown, water-soaked necrotic lesions developed near the soil line. Inoculated plants developed symptoms within 4 days, wilted gradually in 7 days, and all were eventually killed in 11 days. Plants inoculated with sclerotia developed disease at a slower rate and control plants remained symptomless. Sclerotia developed on diseased tissues and S. rolfsii was reisolated. This disease has been observed on many species of plants (2), but to our knowledge, this is the first report of southern blight of silvery messerschmidia seedlings caused by S. rolfsii in Taiwan. References: (1) R. K. Jones and D. M. Benson, eds. Diseases of Woody Ornamentals and Trees in Nurseries. The American Phytopathological Society, St. Paul, MN, 2001. (2) Y. P. Tsai, ed. List of Plant Diseases in Taiwan. The Plant Protection Society of the Republic of China and The Phytopathological Society of the Republic of China, 1991.

scholarly journals First Report of Southern blight of Formosan Michelia Seedlings in Taiwan

Plant Disease ◽  
2006 ◽  
Vol 90 (5) ◽  
pp. 682-682
Author(s):  
W. W. Hsiao ◽  
C. H. Fu ◽  
C. Y. Chen
Keyword(s):  
Plant Protection ◽  
Soil Surface ◽  
Plant Diseases ◽  
First Report ◽  
Southern Blight ◽  
Republic Of China ◽  
Initial Symptoms ◽  
Stem Necrosis ◽  
The Republic Of China ◽  
The Republic

Formosan michelia (Michelia compressa (Maxim.) Sargent) is a native, giant evergreen tree in Taiwan. This species is used for afforestation, provision of shade in domestic situations, used in parks, and also for furniture. During the summer of 2004, a sudden wilt of 1-year-old plants was observed in a nursery of northern Taiwan. Initial symptoms included stem necrosis at the soil line, yellowing, and tan discoloration of leaves. As stem necrosis progressed, infected plants wilted and died. Necrotic tissues were covered with whitish mycelium that differentiated into reddish brown spherical (1 to 2 mm in diameter) sclerotia. Sclerotium rolfsii was consistently recovered from the surface of symptomatic stem sections that were disinfected for 1 min in 0.5% NaOCl and then plated on potato-dextrose agar amended with 100 ppm ampicillin. Pathogenicity of two S. rolfsii isolates was confirmed by inoculating 1-year-old Formosan michelia seedlings grown in pots. Inoculum consisted of mycelium and sclerotia of the pathogen placed on the soil surface around the base of each plant. Noninoculated plants served as controls. All plants were kept in a growth chamber at 25 to 35°C with relative humidity >95%. Inoculated plants developed symptoms within 14 days while control plants remained symptomless. Sclerotia developed on infected tissues, and S. rolfsii was reisolated from symptomatic tissues. This disease has been observed on many species of plants (1), but to our knowledge, this is the first report of southern blight of Formosan michelia seedlings caused by S. rolfsii in Taiwan. Reference: (1) Y. P. Tsai, ed. List of Plant Diseases in Taiwan. The Plant Protection Society of the Republic of China and The Phytopathological Society of the Republic of China, 1991.

scholarly journals First Report of Sclerotium rolfsii on Star-cluster (Pentas lanceolata) in Taiwan

Plant Disease ◽  
2002 ◽  
Vol 86 (11) ◽  
pp. 1275-1275
Author(s):  
C. H. Fu ◽  
H. J. Hsieh ◽  
J. C. Yao
Keyword(s):  
Sclerotium Rolfsii ◽  
Star Cluster ◽  
Plant Diseases ◽  
Pathogenicity Test ◽  
First Report ◽  
Republic Of China ◽  
Stem Necrosis ◽  
The Republic Of China ◽  
The Republic ◽  
Pentas Lanceolata

Star-cluster (Pentas lanceolata (Forssk.) Deflers) has recently become popular as a bedding plant in Taiwan. During the summer of 2000, a sudden wilt of 60-day-old plants was observed in a nursery in Tainan City (southern Taiwan). Initial symptoms included stem necrosis at the soil line and yellowing and tan discoloration of leaves. As stem necrosis progressed, infected plants wilted and died. Necrotic tissues were covered with white mycelium that differentiated into reddish brown, spherical (1 to 2 mm in diameter) sclerotia. Sclerotium rolfsii was consistently recovered from the surface of symptomatic stem sections that were disinfected for 1 min in 0.5% NaOCl and plated on potato dextrose agar amended with 100 ppm streptomycin sulfate. Pathogenicity of three isolates of S. rolfsii was confirmed by inoculating 90-day-old plants of P. lanceolata that were grown in pots. Three plants each were inoculated with a 5-mm plug of agar with mycelium or two sclerotia of the pathogen. Inoculum was placed on the soil surface against the stem of each plant. Three noninoculated plants served as controls. All plants were kept in a growth chamber at 20 to 30°C with relative humidity >85%. The pathogenicity test was repeated. Inoculated plants developed symptoms within 7 days, while control plants remained symptomless. Sclerotia developed on infected tissues and S. rolfsii was reisolated from symptomatic tissues. Although this disease has been observed on many species of plants (1), to our knowledge, this is the first report of southern blight of P. lanceolata caused by S. rolfsii in Taiwan. Reference: (1) Tsai, Y. P., ed. List of Plant Diseases in Taiwan. The Plant Protection Society of the Republic of China and The Phytopathological Society of the Republic of China. 1991.

scholarly journals First Report of Anthracnose Caused by Colletotrichum gloeosporioides on Taxus mairei in Taiwan

Plant Disease ◽  
2003 ◽  
Vol 87 (7) ◽  
pp. 873-873
Author(s):  
C. H. Fu ◽  
W. W. Hsiao ◽  
J. C. Yao
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Plant Protection ◽  
First Record ◽  
Plant Diseases ◽  
Malt Extract ◽  
Forest Protection ◽  
Republic Of China ◽  
Taxus Mairei ◽  
The Republic Of China ◽  
The Republic

Taxus mairei (Lemee & Levl.) S.Y. Hu ex Liu is a giant evergreen tree native to Taiwan. T. mairei and the Pacific yew, T. brevifolia, produce taxol, a highly effective antitumor drug. Anthracnose was observed on cuttings and seedlings of T. mairei in nurseries and on larger plants grown in plantations in Taiwan. Circular or irregular, brown leaf spots were associated with defoliation. Stems lesions and tip dieback were also observed. Colletotrichum gloeosporioides (Penz.) Sacc. was isolated from diseased tissues, and this fungus grew well on potato dextrose agar and malt extract agar (MEA), with a growth rate of 6.3 mm per day on MEA at 32°C in the dark. Colonies were white to grayish white and became dark gray with age. Acervuli produced on leaves were 115 to 155 μm in diameter, with one or several brown-to-black setae and pale salmon conidial masses. Conidia were guttulate, straight, cylindrical, obtuse at the apex and truncate at the base, and approximately 12 to 17 × 3.5 to 6.0 μm. The disease was reproduced by spraying T. mairei seedlings with a suspension of 104 to 105 conidia per ml, and the control plants were inoculated with distilled water. Inoculated plants were kept in a transparent moist chamber with a constant humidity near 100% (1). Symptoms appeared within 7 days when the temperature was over 32°C. When the temperature was below 24°C, symptoms were delayed. The fungus was reisolated from the inoculated plants, fulfilling Koch's postulates. To our knowledge, this is the first record of anthracnose on Taxus mairei (2), and anthracnose appears to be the most important disease on this plant in Taiwan. Voucher specimens have been deposited at the Division of Forest Protection, Taiwan Forest Research Institute as TFRIFCH Herbarium specimen 137. References: (1) W. W. Hsiao et al. Taiwan J. For. Sci.17:119, 2002. (2) Y. P. Tsai, ed. List of plant diseases in Taiwan. The Plant Protection Society of the Republic of China and The Phytopathological Society of the Republic of China, 1991.

scholarly journals First Report of Downy Mildew Caused by Pseudoperonospora cubensis on Chayote (Sechium edule) in Taiwan

Plant Disease ◽  
2008 ◽  
Vol 92 (12) ◽  
pp. 1706-1706 ◽  
Author(s):  
Y. Ko ◽  
C. Y. Chen ◽  
C. W. Liu ◽  
S. S. Chen ◽  
S. Maruthasalam ◽  
...  
Keyword(s):  
Downy Mildew ◽  
Plant Diseases ◽  
Pseudoperonospora Cubensis ◽  
First Report ◽  
Agricultural Improvement ◽  
Republic Of China ◽  
Sechium Edule ◽  
Leaf Surfaces ◽  
Leaf Tissues ◽  
The Republic

Chayote (Sechium edule) is cultivated on more than 500 ha in Taiwan for its edible shoots and fruit. In August 2005 and later, 40 to 75% of the chayote plants cultivated in the Taichung District Agricultural Improvement Station in Puli developed pale yellow, irregular spots on the upper leaf surfaces with corresponding sporulation on the lower leaf surfaces. The lesions eventually became necrotic and spread over the entire leaf surface, leading to defoliation. Pseudoperonospora cubensis, which was previously reported as the cause of downy mildew on squash (Cucurbita moschata), muskmelon (Cucumis melo), cucumber (Cucumis sativus), and sponge gourd (Luffa cylindrica) in Taiwan, was identified (1). Sporangiophores were 182 to 410 μm long, 4.8 to 7.2 μm wide, and dichotomously branched. Sporangia were grayish, ovoid to ellipsoidal, 18.2 to 38.6 μm long, and 13.5 to 25.2 μm wide. Biflagellate zoospores were 9.5 to 12.6 μm in diameter. Pathogenicity tests were conducted four times with six 2-week-old plants in each trial. A sporangial suspension (1 × 105 spores per ml) prepared from infected leaves (5 to 6 weeks after infection) was sprayed on all leaves until runoff. The plants were then covered with polythene bags and incubated for 48 h at 18 ± 1°C in a growth chamber. Control plants were sprayed with sterile water. Characteristic symptoms developed on all inoculated plants after 20 days, while control plants remained symptomless. Microscopic observation of leaf tissues of symptomatic plants confirmed the presence of P. cubensis. To our knowledge, this is the first report of P. cubensis causing downy mildew on chayote in Taiwan. References: (1) S. T. Hsu et al. List of Plant Diseases in Taiwan. The Phytopathological Society of the Republic of China, 2002.

scholarly journals Southern Blight of Areca Palm (Chrysalidocarpus lutescens) Caused by Sclerotium rolfsii in California

Plant Disease ◽  
2005 ◽  
Vol 89 (9) ◽  
pp. 1012-1012
Author(s):  
S. T. Koike
Keyword(s):  
Sclerotium Rolfsii ◽  
Plant Diseases ◽  
Crown Rot ◽  
Peat Moss ◽  
Southern Blight ◽  
Foliage Plants ◽  
Rooting Medium ◽  
Brown Discoloration ◽  
Initial Symptoms ◽  
Rot Disease

Areca palm (Chrysalidocarpus lutescens) is a popular ornamental palm that can be grown outdoors in mild climates and is commonly used as an indoor ornamental plant. During 2005, commercial palm producers lost significant numbers of areca palm seedlings grown in transplant trays to a crown rot disease. Initial symptoms consisted of a light brown discoloration of stems near the soil line. As disease progressed, the brown discoloration extended up the stem and down into the crown, foliage became gray green, and the entire plant then dried up and died. Extensive, white, cottony mycelium and numerous sclerotia developed externally on the lower stem, crown, attached palm seed, and surrounding peat moss medium. Mycelial growth was so extensive that the fungus often grew from one transplant tray cell, bridged across the plastic cell border, and into an adjacent transplant cell. Tan, spherical sclerotia measured approximately 1 mm in diameter. Isolations from diseased plants resulted in the recovery of the same white fungus that produced sclerotia. On the basis of sclerotia morphology and the presence of clamp connections at hyphal septa, the fungus was identified as Sclerotium rolfsii. Pathogenicity was tested by growing isolates on potato dextrose agar, drying the resulting sclerotia for 48 h, and then depositing 8 to 10 sclerotia at the base of healthy areca palm seedlings. Five isolates were tested using 40 plants per isolate. Non-inoculated controls were also included. All plants were incubated in a greenhouse at 22 to 25°C. After 2 weeks, inoculated plants began to show brown necrosis at the base of the stems; by the third week, plants began to dry up, and mycelium and sclerotia developed on the crowns. S. rolfsii was reisolated from all necrotic crown and stem tissues. Noninoculated controls did not develop any disease symptoms. To my knowledge, this is the first report of southern blight of C. lutescens in California. This disease has been reported on areca palms and other foliage plants in the southern United States and Central and South America (1). Circumstantial evidence (the disease occurred on palm seedlings that were planted in previously unused transplant trays and new peat moss rooting medium) suggests that the pathogen may have been brought in on palm seed. In the nursery, other foliage plants that are susceptible to S. rolfsii were planted in the same rooting medium but were unaffected by southern blight. Reference: (1) A. R. Chase. Compendium of Ornamental Foliage Plant Diseases. The American Phytopathological Society. St. Paul, MN, 1987.

scholarly journals First Report of Sclerotium rolfsii on Jerusalem Cherry (Solanum pseudocapsicum) in Europe

Plant Disease ◽  
2000 ◽  
Vol 84 (9) ◽  
pp. 1048-1048
Author(s):  
A. Garibaldi ◽  
A. Minuto ◽  
M. L. Gullino
Keyword(s):  
United States ◽  
Sclerotium Rolfsii ◽  
Soil Surface ◽  
The United States ◽  
Northern Italy ◽  
Ornamental Plant ◽  
Potato Dextrose Agar ◽  
First Report ◽  
Initial Symptoms ◽  
Stem Necrosis

Jerusalem cherry (Solanum pseudocapsicum) has recently become popular as a potted ornamental plant in Italy. During the summer of 1999, a sudden wilt of 60-day-old plants was observed in the Albenga region (Northern Italy), an area of intensive floriculture. Initial symptoms included stem necrosis at the soil line and yellowing and tan discoloration of leaves. As stem necrosis progressed, infected plants wilted and died. Necrotic tissues were covered with whitish mycelium that differentiated into reddish brown, spherical (1 to 2 mm diameter) sclerotia. Sclerotium rolfsii was consistently recovered from the surface of symptomatic stem sections that were disinfected for 1 min in 1% NaOCl and then plated on potato-dextrose agar (PDA) amended with 100 ppm streptomycin sulfate. Pathogenicity of three S. rolfsii isolates was confirmed by inoculating 90-day-old S. pseudocapsicum plants grown in pots. Inoculum consisted of mycelium and sclerotia of the pathogen placed on the soil surface around the base of each plant. Noninoculated plants served as controls. All plants were kept in a growth chamber at 18 to 28°C and RH > 85%. Inoculated plants developed symptoms within 7 days, while control plants remained symptomless. Sclerotia developed on infected tissues and S. rolfsii was reisolated from symptomatic tissues. The disease has been observed in the United States (1), but this is the first report of stem blight of S. pseudocapsicum caused by S. rolfsii in Europe. Reference: (1) S. A. Alfieri, Jr., K. R. Langdon, C. Wehlburg, and J. W. Kimbrough, J. W. Index Plant Dis. Florida Bull. 11:215, 1984.

The Normalization of Sino-French Diplomatic Relations in 1964 and the Formation of the “One-China” Principle: Negotiations over Breaking French Diplomatic Relations with the Republic of China Government and the Recognition of the People’s Republic of China as the Sole Legitimate Government

2012 ◽  
Vol 8 (1) ◽  
pp. 252-271
Author(s):  
Madoka Fukuda
Keyword(s):  
The United States ◽  
People’S Republic Of China ◽  
Diplomatic Relations ◽  
People's Republic Of China ◽  
African Countries ◽  
Republic Of China ◽  
The Republic Of China ◽  
The Republic ◽  
The Government ◽  
The One

AbstractThis article examines the substance and modification of the “One-China” principle, which the government of the People’s Republic of China (PRC) pursued in the mid 1960s. Under this principle, a country wishing to establish diplomatic relations with the PRC was required first to break off such relations with the Republic of China (ROC). In 1964 the PRC established diplomatic relations with France. This was its first ambassadorial exchange with a Western government. The PRC, in the negotiations over the establishment of diplomatic relations, attempted to achieve some consensus with France on the matter of “One-China”. The PRC, nevertheless, had to abandon these attempts, even though it demanded fewer conditions of France than of the United States (USA), Japan and other Western countries in the 1970s. The PRC had demanded adherence to the “One-China” principle since 1949. France, however, refused to accept this condition. Nevertheless, the PRC established diplomatic relations with France before the latter broke off relations with the ROC. Subsequently, the PRC abandoned the same condition in negotiations with the African governments of the Republic of Congo, Central Africa, Dahomey and Mauritania. After the negotiations with France, the PRC began to insist that the joint communiqué on the establishment of diplomatic relations should clearly state that “the Government of the People’s Republic of China is the sole legal government of China”. However, France refused to insert these words into the communiqué. Afterwards, the PRC nevertheless insisted on putting such a statement into the joint communiqués or exchanges of notes on the establishment of diplomatic relations with the African countries mentioned above. This was done in order to set precedents for making countries accede to the “One-China” principle. The “One-China” principle was, thus, gradually formed in the process of the negotiation and bargaining between the PRC and other governments.

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