scholarly journals Colonic Crypt‐Derived 3D Gut Organoid from Hypertensive Rat Maintain In vivo Characteristics

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Jing li ◽  
Qing Su ◽  
Marianthi M. Karas ◽  
Elaine M. Richards ◽  
Mohan K. Raizada
Keyword(s):  
Colonic Crypt ◽  
Hypertensive Rat

Histamine-induced protein leakage in hypertensive rats: inhibition by verapamil

1986 ◽  
Vol 250 (2) ◽  
pp. H284-H290 ◽  
Author(s):  
F. N. Miller ◽  
I. G. Joshua ◽  
J. T. Fleming ◽  
N. Parekh
Keyword(s):  
Fluorescent Microscopy ◽  
Fluorescein Isothiocyanate ◽  
Calcium Entry Blocker ◽  
Hypertensive Rats ◽  
Rat Serum ◽  
Protein Leakage ◽  
Hypertensive Rat ◽  
Increased Sensitivity ◽  
Microscopy Techniques

Hypertension has been associated with an enhanced transport of macromolecules from the vasculature to the interstitium. The first objective of this study was to determine if, under control conditions, there is an enhanced leakage of macromolecules from the cremaster vasculature of the hypertensive rat. The second objective was to determine if the response to a mediator of macromolecular leakage (histamine) was altered in the renovascular hypertensive rat. A third objective was to determine if a calcium entry blocker, verapamil, could inhibit histamine-induced leakage and, if so, was the sensitivity to verapamil different in the renovascular hypertensive rat. Rats were anesthetized with pentobarbital, and the cremaster preparation was used for in vivo television microscopy studies. Fluorescein isothiocyanate was tagged to rat serum albumin (FITC-RSA), and the leakage of this albumin from the vasculature to the interstitium was quantitated by the use of fluorescent microscopy techniques. There was no difference during control conditions in macromolecular leakage between the normotensive and hypertensive rats. However, histamine induced a greater leakage in the renovascular hypertensive rat than in the normotensive controls. In addition, verapamil, in the presence of normal calcium levels, inhibited the histamine-induced leakage in the hypertensive rats but not in the normotensive controls. These data suggest that enhanced macromolecular leakage during hypertension may be due to an increased sensitivity to mediators of protein leakage. These agents may produce protein leakage by enhancing entry of extracellular calcium into endothelial cells.

scholarly journals In vivo alterations in cardiac metabolism and function in the spontaneously hypertensive rat heart

2012 ◽  
Vol 95 (1) ◽  
pp. 69-76 ◽  
Author(s):  
M. S. Dodd ◽  
D. R. Ball ◽  
M. A. Schroeder ◽  
L. M. Le Page ◽  
H. J. Atherton ◽  
...  
Keyword(s):  
Rat Heart ◽  
Spontaneously Hypertensive Rat ◽  
Cardiac Metabolism ◽  
Spontaneously Hypertensive ◽  
Hypertensive Rat ◽  
And Function

Modification of renin isoelectric heterogeneity in Goldblatt hypertensive rat

1985 ◽  
Vol 248 (6) ◽  
pp. E694-E698 ◽  
Author(s):  
F. M. Sessler ◽  
R. L. Malvin
Keyword(s):  
Isoelectric Focusing ◽  
Rat Kidney ◽  
Venous Blood ◽  
Renin Activity ◽  
Hypertensive Rat ◽  
Isoelectric Points ◽  
The Difference ◽  
Cortical Slices

Six forms of renin have been described in rat kidney. Different stimuli resulted in secretion of unique profiles of those forms. We studied their storage and secretion in the two-kidney, one-clip Goldblatt hypertensive rat (GHR). Renal venous blood, kidney homogenates, and incubation media from cortical slices were subjected to isoelectric focusing. In all samples tested, six peaks of renin activity were found with isoelectric points at pH 5.90, 5.70, 5.40, 5.20, 5.00, and 4.80. The quantity of renin activity for each form was expressed as a percentage of the total recovered from the gel. In control kidneys the profile of renin stored and that released by in vitro slices were similar. However, in plasma, the percentage of renin focusing at the more basic pH was decreased. This is in agreement with other work showing that the liver removes the more basic forms more rapidly than the acidic forms. The clipped kidney of GHR secreted, both in vivo and in vitro, a profile of renin forms that was significantly different from the control kidney. The difference was expressed by an increase in the secretion of the more acidic forms by the clipped kidney. It is hypothesized that changes in the secretory profile of renin may reflect changes in storage and synthesis of those forms.

Helium-induced cardioprotection of healthy and hypertensive rat myocardium in vivo

2012 ◽  
Vol 684 (1-3) ◽  
pp. 125-131 ◽  
Author(s):  
Gezina T.M.L. Oei ◽  
Ragnar Huhn ◽  
Andre Heinen ◽  
Markus W. Hollmann ◽  
Wolfgang S. Schlack ◽  
...  
Keyword(s):  
Rat Myocardium ◽  
Hypertensive Rat
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