scholarly journals Human Embryonic Stem Cells in Culture Possess Primary Cilia with Hedgehog Signaling Machinery

2008 ◽  
Vol 22 (S1) ◽  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Hedgehog Signaling ◽  
Primary Cilia ◽  
Embryonic Stem ◽  
Cells In Culture

scholarly journals Human embryonic stem cells in culture possess primary cilia with hedgehog signaling machinery

2008 ◽  
Vol 180 (5) ◽  
pp. 897-904 ◽  
Author(s):  
Enko N. Kiprilov ◽  
Aashir Awan ◽  
Romain Desprat ◽  
Michelle Velho ◽  
Christian A. Clement ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Hedgehog Signaling ◽  
Primary Cilia ◽  
Embryonic Stem ◽  
Serum Starvation ◽  
Mechanistic Basis ◽  
Patched 1 ◽  
Hesc Lines

Human embryonic stem cells (hESCs) are potential therapeutic tools and models of human development. With a growing interest in primary cilia in signal transduction pathways that are crucial for embryological development and tissue differentiation and interest in mechanisms regulating human hESC differentiation, demonstrating the existence of primary cilia and the localization of signaling components in undifferentiated hESCs establishes a mechanistic basis for the regulation of hESC differentiation. Using electron microscopy (EM), immunofluorescence, and confocal microscopies, we show that primary cilia are present in three undifferentiated hESC lines. EM reveals the characteristic 9 + 0 axoneme. The number and length of cilia increase after serum starvation. Important components of the hedgehog (Hh) pathway, including smoothened, patched 1 (Ptc1), and Gli1 and 2, are present in the cilia. Stimulation of the pathway results in the concerted movement of Ptc1 out of, and smoothened into, the primary cilium as well as up-regulation of GLI1 and PTC1. These findings show that hESCs contain primary cilia associated with working Hh machinery.

scholarly journals Notch and Hedgehog Signaling Cooperate to Maintain Self-Renewal of Human Embryonic Stem Cells Exposed to Low Oxygen Concentration

2010 ◽  
Vol 3 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Simon C. Weli ◽  
Trine Fink ◽  
Cihan Cetinkaya ◽  
Mayuri S. Prasad ◽  
Cristian P. Pennisi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Oxygen Concentration ◽  
Human Embryonic Stem Cells ◽  
Hedgehog Signaling ◽  
Embryonic Stem ◽  
Low Oxygen ◽  
Self Renewal ◽  
Low Oxygen Concentration

scholarly journals Role of Sonic hedgehog signaling and the expression of its components in human embryonic stem cells

2010 ◽  
Vol 4 (1) ◽  
pp. 38-49 ◽  
Author(s):  
Selena Meiyun Wu ◽  
Andre B.H. Choo ◽  
Miranda G.S. Yap ◽  
Ken Kwok-Keung Chan
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Sonic Hedgehog ◽  
Human Embryonic Stem Cells ◽  
Hedgehog Signaling ◽  
Embryonic Stem ◽  
Sonic Hedgehog Signaling

scholarly journals TP53 promotes lineage commitment of human embryonic stem cells through ciliogenesis and sonic hedgehog signaling

2021 ◽  
Author(s):  
Sushama Sivakumar ◽  
Shutao Qi ◽  
Ningyan Cheng ◽  
Adwait amod sathe ◽  
Mohammed Kanchwala ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Sonic Hedgehog ◽  
Human Embryonic Stem Cells ◽  
Hedgehog Signaling ◽  
Neural Progenitor Cells ◽  
Embryonic Stem ◽  
Low Grade ◽  
Shh Signaling

Aneuploidy, defective differentiation, and inactivation of the tumor suppressor TP53 all occur frequently during tumorigenesis. Here, we probe the potential links among these cancer traits by inactivating TP53 in human embryonic stem cells (hESCs). TP53 knockout hESCs exhibit increased proliferation rates, mitotic errors, and low-grade structural aneuploidy; produce poorly differentiated immature teratomas in mice; and fail to differentiate into neural progenitor cells (NPC) in vitro. Genome-wide CRISPR screen reveals requirements of ciliogenesis and sonic hedgehog (Shh) pathways for hESC differentiation into NPCs. TP53 deletion causes abnormal ciliogenesis in neural rosettes. In addition to restraining cell proliferation through CDKN1A, TP53 activates the transcription of BBS9, which encodes a ciliogenesis regulator required for proper Shh signaling and NPC formation. This developmentally regulated transcriptional program of TP53 promotes ciliogenesis, restrains Shh signaling, and commits hESCs to neural lineages.

scholarly journals Human Adult Marrow Cells Support Prolonged Expansion of Human Embryonic Stem Cells in Culture

Stem Cells ◽  
2003 ◽  
Vol 21 (2) ◽  
pp. 131-142 ◽  
Author(s):  
Linzhao Cheng ◽  
Holly Hammond ◽  
Zhaohui Ye ◽  
Xiangcan Zhan ◽  
Gautam Dravid
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Cells In Culture ◽  
Human Adult ◽  
Marrow Cells
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