scholarly journals Trypanosoma cruzi regulates the defensin alpha‐1 interactome network and defensin alpha‐1 causes trypanosome membrane pore formation to control the early process of infection

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Vyacheslav Furtak ◽  
Yuliya Kleshchenko ◽  
Pius Nde ◽  
Siddarth Pratap ◽  
Candice Johnson ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Pore Formation ◽  
Membrane Pore ◽  
Interactome Network

scholarly journals Human Defensin α-1 Causes Trypanosoma cruzi Membrane Pore Formation and Induces DNA Fragmentation, Which Leads to Trypanosome Destruction

2007 ◽  
Vol 75 (10) ◽  
pp. 4780-4791 ◽  
Author(s):  
M. Nia Madison ◽  
Yuliya Y. Kleshchenko ◽  
Pius N. Nde ◽  
Kaneatra J. Simmons ◽  
Maria F. Lima ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Dna Fragmentation ◽  
Pore Formation ◽  
Microscopic Analysis ◽  
Innate Immune ◽  
Microbial Pathogens ◽  
Severe Toxicity ◽  
Electron Microscopic ◽  
Membrane Pore ◽  
Immune Molecule

ABSTRACT Human defensins play a fundamental role in the initiation of innate immune responses to some microbial pathogens. Here we show that human defensin α-1 displays a trypanocidal role against Trypanosoma cruzi, the causative agent of Chagas' disease. The toxicity of human defensin α-1 against T. cruzi is mediated by membrane pore formation and the induction of nuclear and mitochondrial DNA fragmentation, leading to trypanosome destruction. Exposure of trypomastigote and amastigote forms of T. cruzi to defensin α-1 significantly reduced parasite viability in a peptide concentration-dependent and saturable manner. The toxicity of defensin α-1 against T. cruzi is blocked by anti-defensin α-1 immunoglobulin G. Electron microscopic analysis of trypomastigotes exposed to defensin α-1 revealed pore formation in the cellular and flagellar membranes, membrane disorganization, and blebbing as well as cytoplasmic vacuolization. Furthermore, human defensin α-1 enters the trypanosome when membrane pores are present and is associated with later intracellular damage. Trypanosome membrane depolarization abolished the toxicity of defensin α-1 against the parasite. Preincubation of trypomastigotes with defensin α-1 followed by exposure to human epithelial cells significantly reduced T. cruzi infection in these cells. Thus, human defensin α-1 is an innate immune molecule that causes severe toxicity to T. cruzi and plays an important role in reducing cellular infection. This is the first report showing that human defensin α-1 causes membrane pore formation in a human parasite, leading to trypanosome destruction.

scholarly journals Human defensin alpha‐1 causes Trypanosoma cruzi membrane pore formation and induces DNA fragmentation, which leads to trypanosome destruction

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Marisa Nia Madison ◽  
YY Kleshchenko ◽  
PN Nde ◽  
KJ Simmons ◽  
MF Lima ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Dna Fragmentation ◽  
Pore Formation ◽  
Membrane Pore

Membrane pore formation at protein–lipid interfaces

2014 ◽  
Vol 39 (11) ◽  
pp. 510-516 ◽  
Author(s):  
Robert J.C. Gilbert ◽  
Mauro Dalla Serra ◽  
Christopher J. Froelich ◽  
Mark I. Wallace ◽  
Gregor Anderluh
Keyword(s):  
Pore Formation ◽  
Membrane Pore

scholarly journals Molecular Basis for Membrane Pore Formation by Bax Protein Carboxyl Terminus

Biochemistry ◽  
2012 ◽  
Vol 51 (46) ◽  
pp. 9406-9419 ◽  
Author(s):  
Suren A. Tatulian ◽  
Pranav Garg ◽  
Kathleen N. Nemec ◽  
Bo Chen ◽  
Annette R. Khaled
Keyword(s):  
Molecular Basis ◽  
Pore Formation ◽  
Carboxyl Terminus ◽  
Membrane Pore ◽  
Bax Protein

Mechanisms of Membrane Pore Formation by Amyloidogenic Peptides in Amyotrophic Lateral Sclerosis

2016 ◽  
Vol 22 (29) ◽  
pp. 9958-9961 ◽  
Author(s):  
Charles H. Chen ◽  
Ayesha Khan ◽  
Joseph Jen-Tse Huang ◽  
Martin B. Ulmschneider
Keyword(s):  
Amyotrophic Lateral Sclerosis ◽  
Pore Formation ◽  
Membrane Pore ◽  
Amyloidogenic Peptides ◽  
Lateral Sclerosis

scholarly journals Mechanism of membrane pore formation by human gasdermin‐D

2018 ◽  
Vol 37 (14) ◽  
Author(s):  
Estefania Mulvihill ◽  
Lorenzo Sborgi ◽  
Stefania A Mari ◽  
Moritz Pfreundschuh ◽  
Sebastian Hiller ◽  
...  
Keyword(s):  
Pore Formation ◽  
Membrane Pore

scholarly journals Functional Assay to Correlate Protein Oligomerization States with Membrane Pore Formation

2020 ◽  
Vol 92 (22) ◽  
pp. 14861-14866
Author(s):  
Radek Šachl ◽  
Sabína Čujová ◽  
Vandana Singh ◽  
Petra Riegerová ◽  
Peter Kapusta ◽  
...  
Keyword(s):  
Pore Formation ◽  
Functional Assay ◽  
Protein Oligomerization ◽  
Membrane Pore

scholarly journals Medin Oligomer Membrane Pore Formation: A Potential Mechanism of Vascular Dysfunction

2020 ◽  
Vol 118 (11) ◽  
pp. 2769-2782 ◽  
Author(s):  
Scott Younger ◽  
Hyunbum Jang ◽  
Hannah A. Davies ◽  
Martin J. Niemiec ◽  
Joe G.N. Garcia ◽  
...  
Keyword(s):  
Pore Formation ◽  
Vascular Dysfunction ◽  
Potential Mechanism ◽  
Membrane Pore
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