Signal transduction and intracellular signaling in pancreatic acinar cells

1995 ◽  
Vol 11 (5) ◽  
pp. 397-401 ◽  
Author(s):  
John A. Williams
Keyword(s):  
Signal Transduction ◽  
Intracellular Signaling ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells

Adenovirus-mediated gene transfer of RasN17 inhibits specific CCK actions on pancreatic acinar cells

1999 ◽  
Vol 276 (2) ◽  
pp. G499-G506 ◽  
Author(s):  
Barbara Nicke ◽  
Min-Jen Tseng ◽  
Marycarol Fenrich ◽  
Craig D. Logsdon
Keyword(s):  
Dna Synthesis ◽  
Intracellular Signaling ◽  
Acinar Cells ◽  
Dominant Negative ◽  
Pancreatic Acinar Cells ◽  
Dominant Negative Mutant ◽  
Ras Gene ◽  
Amylase Release ◽  
Jun Kinase

CCK stimulates pleiotrophic responses in pancreatic acinar cells; however, the intracellular signaling pathways involved are not well understood. To evaluate the role of the ras gene product in CCK actions, a strategy involving in vitro adenoviral-mediated gene delivery of a dominant-negative mutant Ras (RasN17) was utilized. Isolated acini were infected with various titers of either a control adenovirus or an adenoviral construct expressing RasN17 for 24 h before being treated with CCK. Titer-dependent expression of RasN17 in the acini was confirmed by Western blotting. Infection with control adenovirus [106–109plaque-forming units/mg acinar protein (multiplicity of infection of ∼1–1,000)] had no effect on CCK stimulation of acinar cell amylase release, extracellular-regulated kinase (ERK) or c-Jun kinase (JNK) kinases, or DNA synthesis. In contrast, infection with adenovirus bearing ras N17 increased basal amylase release, inhibited CCK-mediated JNK activation, had no effect on CCK activation of ERK, and inhibited DNA synthesis. These data demonstrate important roles for Ras in specific actions of CCK on pancreatic acinar function.

scholarly journals Free radicals and the pancreatic acinar cells: role in physiology and pathology

2005 ◽  
Vol 360 (1464) ◽  
pp. 2273-2284 ◽  
Author(s):  
M Chvanov ◽  
O.H Petersen ◽  
A Tepikin
Keyword(s):  
Nitric Oxide ◽  
Signal Transduction ◽  
Acute Pancreatitis ◽  
Vascular Endothelium ◽  
Cell Injury ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells ◽  
Nitrogen Species ◽  
Key Enzyme ◽  
Oxide Synthase

Reactive oxygen and nitrogen species (ROS and RNS) play an important role in signal transduction and cell injury processes. Nitric oxide synthase (NOS)—the key enzyme producing nitric oxide (NO)—is found in neuronal structures, vascular endothelium and, possibly, in acinar and ductal epithelial cells in the pancreas. NO is known to regulate cell homeostasis, and its effects on the acinar cells are reviewed here. ROS are implicated in the early events within the acinar cells, leading to the development of acute pancreatitis. The available data on ROS/RNS involvement in the apoptotic and necrotic death of pancreatic acinar cells will be discussed.

Effects of guanine nucleotides on bombesin-stimulated signal transduction in rat pancreatic acinar cells

1993 ◽  
Vol 193 (1) ◽  
pp. 323-335 ◽  
Author(s):  
Albrecht Piiper ◽  
Danuta Stryjek-Kaminska ◽  
Jürgen Stein ◽  
Wolfgang F. Caspary ◽  
Stefan Zeuzem
Keyword(s):  
Signal Transduction ◽  
Acinar Cells ◽  
Guanine Nucleotides ◽  
Pancreatic Acinar Cells

scholarly journals Signal transduction of MCP-1 expression induced by pancreatitis-associated ascitic fluid in pancreatic acinar cells

2009 ◽  
Vol 13 (7) ◽  
pp. 1314-1320 ◽  
Author(s):  
Laura Ramudo ◽  
Sara Yubero ◽  
Manuel A. Manso ◽  
Secundino Vicente ◽  
Isabel De Dios
Keyword(s):  
Signal Transduction ◽  
Ascitic Fluid ◽  
Acinar Cells ◽  
Pancreatic Acinar Cells
Sign in / Sign up

Export Citation Format

Share Document