scholarly journals Activity of Mitochondrial Respiratory Chain Enzymes after Transient Focal Ischemia in the Rat

1997 ◽  
Vol 17 (11) ◽  
pp. 1166-1169 ◽  
Author(s):  
Laura Canevari ◽  
Satoshi Kuroda ◽  
Timothy E. Bates ◽  
John B. Clark ◽  
Bo K. Siesjö
Keyword(s):  
Respiratory Chain ◽  
Citrate Synthase ◽  
Mitochondrial Respiratory Chain ◽  
Artery Occlusion ◽  
Respiratory Chain Complexes ◽  
Secondary Damage ◽  
Chain Complexes ◽  
Postischemic Recirculation ◽  
Transient Focal Ischemia ◽  
Mitochondrial Respiratory

Previous results demonstrated that after 2-hour middle cerebral artery occlusion (MCAO) in the rat, 1- to 2-hour recirculation temporarily restored the bioenergetic state and mitochondrial function, but secondary deterioration took place after 4 hours. The authors measured the activity of mitochondrial respiratory chain complexes, citrate synthase, and glutamate dehydrogenase as possible targets of secondary damage. Focal and penumbral tissues were sampled in the control condition, after 2 hours of MCAO, and after 1, 2, or 4 hours of postischemic recirculation; two groups were treated with α-phenyl-N- tert-butyl-nitrone (PBN). Complex IV activity transiently decreased after MCAO, but after recirculation all measured activities returned to control values.

scholarly journals Mitochondrial Respiratory Chain and Its Regulatory Elements SIRT1 and SIRT3 Play Important Role in the Initial Process of Energy Conversion after Moxibustion at Local Skin

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Ning Zhang ◽  
Na Zhao ◽  
Lu-shuang Xie ◽  
Biao Huang ◽  
Si-rui Lin ◽  
...  
Keyword(s):  
Energy Conversion ◽  
Respiratory Chain ◽  
Thermal Energy ◽  
Mitochondrial Respiratory Chain ◽  
Regulatory Elements ◽  
Respiratory Chain Complexes ◽  
Local Skin ◽  
Chain Complexes ◽  
Initial Process ◽  
Mitochondrial Respiratory

Objectives. To study how thermal energy is converted after moxibustion at local skin from the view of mitochondrial respiratory chain and its key regulatory elements of sirtuins 1 (SIRT1) and sirtuins 3 (SIRT3). Methods. Two moxibustion temperatures usually used in clinical practice (38°C and 46°C) were applied to Zusanli (ST36) acupoint for 30 minutes in C57BL/6J mice. Local skin samples were harvested at 30 min and 72 h after moxibustion intervention, respectively. The activity of mitochondrial respiratory chain complexes I–V was detected by spectrophotometry. The expression of SIRT1 and SIRT3 protein was detected by immunofluorescence staining or western blot. Results. Moxibustion at 38°C triggered more significant increase of mitochondrial respiratory chain complexes I–V expression. However, the protein expression of SIRT1 and SIRT3 at 46°C showed more obvious enhancement. In addition, the effect of mitochondrial respiratory chain complexes I–V activity on local skin of ST36 acupoint was more obvious at 30 min after moxibustion, while the expression of SIRT1 and SIRT3 protein was more significant at 72 h after moxibustion. Conclusion. Mitochondrial respiratory chain and its key regulatory element proteins SIRT1 and SIRT3 play important role in the initial process of thermal energy conversion stimulated by different moxibustion temperatures in local skin.

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