Routine Methods in Toxicology and Therapeutic Drug Monitoring by High-Performance Liquid Chromatography. I. Rapid Method for Determination of Acetaminophen in Plasma, Including a STAT Procedure

1987 ◽  
Vol 9 (2) ◽  
pp. 248 ◽  
Author(s):  
S. Paul Sood ◽  
Victor I. Green
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Therapeutic Drug Monitoring ◽  
Rapid Method ◽  
Drug Monitoring ◽  
High Performance ◽  
Therapeutic Drug

scholarly journals Simultaneous Determination of 12 β-Lactam Antibiotics in Human Plasma by High-Performance Liquid Chromatography with UV Detection: Application to Therapeutic Drug Monitoring

2011 ◽  
Vol 55 (10) ◽  
pp. 4873-4879 ◽  
Author(s):  
Marie-Clémence Verdier ◽  
Olivier Tribut ◽  
Pierre Tattevin ◽  
Yves Le Tulzo ◽  
Christian Michelet ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Therapeutic Drug Monitoring ◽  
Simultaneous Determination ◽  
Drug Monitoring ◽  
High Performance ◽  
Therapeutic Drug ◽  
Beta Lactam ◽  
Beta Lactam Antibiotics

ABSTRACTA rapid and specific high-performance liquid chromatography method with UV detection (HPLC-UV) for the simultaneous determination of 12 beta-lactam antibiotics (amoxicillin, cefepime, cefotaxime, ceftazidime, ceftriaxone, cloxacillin, imipenem, meropenem, oxacillin, penicillin G, piperacillin, and ticarcillin) in small samples of human plasma is described. Extraction consisted of protein precipitation by acetonitrile. An Atlantis T3 analytical column with a linear gradient of acetonitrile and a pH 2 phosphoric acid solution was used for separation. Wavelength photodiode array detection was set either at 210 nm, 230 nm, or 298 nm according to the compound. This method is accurate and reproducible (coefficient of variation [CV] < 8%), allowing quantification of beta-lactam plasma levels from 5 to 250 μg/ml without interference with other common drugs. This technique is easy to use in routine therapeutic drug monitoring of beta-lactam antibiotics.

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