High-Performance Liquid Chromatography Determination of Dapsone, Monoacetyldapsone, and Pyrimethamine in Filter Paper Blood Spots

1995 ◽  
Vol 17 (1) ◽  
pp. 79-83 ◽  
Author(s):  
A. M. Ronn ◽  
M. M. Lemnge ◽  
H. R. Angelo ◽  
I. C. Bygbjerg
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Filter Paper ◽  
High Performance ◽  
Blood Spots

Determination of vanillylmandelic acid, vanillactic acid, and homovanillic acid in dried urine on filter-paper discs by high-performance liquid chromatography with coulometric electrochemical detection for neuroblastoma screening.

1988 ◽  
Vol 34 (11) ◽  
pp. 2228-2230 ◽  
Author(s):  
Y Kinoshita ◽  
S Yamada ◽  
K Haraguchi ◽  
T Takayanagi ◽  
Y Mori ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrochemical Detection ◽  
Filter Paper ◽  
High Performance ◽  
Homovanillic Acid ◽  
Vanillylmandelic Acid ◽  
Chromatographic Procedure ◽  
One Year

Abstract We report a method for determination of vanillylmandelic acid, vanillactic acid, and homovanillic acid by high-performance liquid chromatography (HPLC), with coulometric electrochemical detection, for mass screening of neuroblastoma. Urine samples were collected on filter paper, dried, and then pretreated. The chromatographic procedure is reliable and fast, allowing for a large sample throughput for routine screening. Intricate extraction procedures and centrifugal separation are unnecessary. Screening for neuroblastoma by HPLC is rapidly gaining acceptance in Japan, and our method is being used at many screening centers. Of 26,571 infants screened in one year in Yokohama City, our method detected five with neuroblastoma.

scholarly journals Determination of Efavirenz in Human Dried Blood Spots by Reversed-phase High-performance Liquid Chromatography With UV Detection

2013 ◽  
Vol 35 (2) ◽  
pp. 203-208 ◽  
Author(s):  
Justin T. Hoffman ◽  
Steven S. Rossi ◽  
Rowena Espina-Quinto ◽  
Scott Letendre ◽  
Edmund V. Capparelli
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Dried Blood Spots ◽  
Reversed Phase ◽  
Uv Detection ◽  
Blood Spots

Phenylalanine analyses of blood-spot control materials: preparation of samples and evaluation of interlaboratory performance.

1985 ◽  
Vol 31 (2) ◽  
pp. 235-238 ◽  
Author(s):  
F W Spierto ◽  
T L Hearn ◽  
F H Gardner ◽  
W H Hannon
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Filter Paper ◽  
Whole Blood ◽  
High Performance ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Interlaboratory Studies ◽  
Blood Spot ◽  
Materials Preparation

Abstract Aliquots (0.1 mL) of whole-blood pools prepared to contain various concentrations of phenylalanine were applied to filter-paper collection cards, dried, and stored in sealed bags. We measured the phenylalanine content of the dried blood spots by bioassay, fluorometry, and "high-performance" liquid chromatography, and found that the concentrations remained constant for two years when samples were kept at -20 degrees C or lower. Intra- and interlaboratory studies showed that results for phenylalanine were greater for laboratories using bioassay procedures than for those using fluorometric procedures. Further, CVs (both among- and within-laboratory) obtained with fluorometric procedures were nearly half as great as the CVs obtained by laboratories using bioassay techniques.

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