DONOR SELECTION FOR BONE MARROW TRANSPLANTATION PREDICTIVE VALUE OF DR TYPING FOR MIXED LYMPHOCYTE CULTURE COMPATIBILITY BETWEEN UNRELATED INDIVIDUALS

1978 ◽  
Vol 26 (6) ◽  
pp. 448-450 ◽  
Author(s):  
&NA;
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Predictive Value ◽  
Marrow Transplantation ◽  
Mixed Lymphocyte Culture ◽  
Lymphocyte Culture ◽  
Donor Selection ◽  
Selection For

BONE MARROW TRANSPLANTATION BETWEEN MIXED LYMPHOCYTE CULTURE-REACTIVE INDIVIDUALS

1975 ◽  
Vol 20 (3) ◽  
pp. 194-197 ◽  
Author(s):  
ROBERT PETER GALE ◽  
GERHARD OPELZ ◽  
ROBERT SPARKES ◽  
THE UCLA BONE
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Marrow Transplantation ◽  
Mixed Lymphocyte Culture ◽  
Lymphocyte Culture

scholarly journals Successful bone marrow transplantation against mixed lymphocyte culture barrier

Blood ◽  
1976 ◽  
Vol 48 (3) ◽  
pp. 385-391
Author(s):  
SA Feig ◽  
G Opelz ◽  
HS Winter ◽  
PM Falk ◽  
RC Neerhout ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Aplastic Anemia ◽  
Genetic Markers ◽  
Marrow Transplantation ◽  
Mixed Lymphocyte Culture ◽  
Lymphocyte Culture ◽  
Host Disease ◽  
The Face

If bone marrow transplantation is to become widely applicable in the treatment of patients with leukemia and aplastic anemia, the necessity to have a perfectly histocompatible donor must be overcome. In an effort to define the roles of HL-A type and mixed lymphocyte culture (MLC) reactivity in the determination of successful engraftment and the occurrence of graftversus-host disease (GVHD), we have attempted transplantation of a child with acute myeloblastic leukemia (AML) using an HL-A identical, MLC-reactive sibling donor. Successful engraftment has been accomplished, as documented by the appearance of multiple donor genetic markers in the recipient. There is no evidence of severe GVHD. The recipient is alive, without evidence of leukemia, and has returned to full activities 9 mo after transplantation. The recipient now produces lymphocytes, which have the MLC reactivity that characterize the donor's lymphocytes, rather than that of her own pretransplant lymphocytes. This experience demonstrates that successful bone marrow transplantation in patients with leukemia can be accomplished in the face of MLC reactivity.

scholarly journals Addition of exogenous cytokines in mixed lymphocyte culture for selecting related donors for bone marrow transplantation

2002 ◽  
Vol 120 (6) ◽  
pp. 175-179
Author(s):  
Jeane Eliete Laguila Visentainer ◽  
Sofia Rocha Lieber ◽  
Lígia Beatriz Lopes Persoli ◽  
Afonso Celso Vigorito ◽  
Francisco José Penteado Aranha ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Marrow Transplantation ◽  
Recipient Cell ◽  
Mixed Lymphocyte Culture ◽  
Lymphocyte Culture ◽  
Unrelated Control ◽  
Significant Difference ◽  
Lymphocyte Cultures ◽  
Mixed Lymphocyte Cultures

CONTEXT: Mixed lymphocyte culturing has led to conflicting opinions regarding the selection of donors for bone marrow transplantation. The association between a positive mixed lymphocyte culture and the development of graft-versus-host disease (GVHD) is unclear. The use of exogenous cytokines in mixed lymphocyte cultures could be an alternative for increasing the sensitivity of culture tests. OBJECTIVE: To increase the sensitivity of mixed lymphocyte cultures between donor and recipient human leukocyte antigen (HLA) identical siblings, using exogenous cytokines, in order to predict post-transplantation GVHD and/or rejection. TYPE OF STUDY: Prospective study. SETTING: Bone Marrow Transplantation Unit, Universidade Estadual de Campinas. PARTICIPANTS: Seventeen patients with hematological malignancies and their respective donors selected for bone marrow transplantation procedures. PROCEDURES: Standard and modified mixed lymphocyte culturing by cytokine supplementation was carried out using donor and recipient cells typed for HLA. MAIN MEASUREMENTS: Autologous and allogenic responses in mixed lymphocyte cultures after the addition of IL-4 or IL-2. RESULTS: In comparison with the standard method, average responses in the modified mixed lymphocyte cultures increased by a factor of 2.0 using IL-4 (p < 0.001) and 6.4 using IL-2 (p < 0.001), for autologous donor culture responses. For donor-versus-recipient culture responses, the increase was by a factor of 1.9 using IL-4 (p < 0.001) and 4.1 using IL-2 (p < 0.001). For donor-versus-unrelated culture responses, no significant increase was observed using IL-4, and a mean response inhibition of 20% was observed using IL-2 (p < 0.001). Neither of the cytokines produced a significant difference in the unrelated control versus recipient cell responses. CONCLUSION: IL-4 supplementation was the best for increasing the mixed lymphocyte culture sensitivity. However, IL-4 also increased autologous responses, albeit less intensively than IL-2. Thus, with this loss of specificity we believe that it is not worth modifying the traditional mixed lymphocyte culture method, even with IL-4 addition.

scholarly journals Bone marrow transplantation for constitutional pure red cell aplasia

Blood ◽  
1988 ◽  
Vol 71 (1) ◽  
pp. 226-229 ◽  
Author(s):  
C Lenarsky ◽  
K Weinberg ◽  
E Guinan ◽  
PP Dukes ◽  
Y Barak ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Marrow Transplantation ◽  
Pure Red Cell Aplasia ◽  
Mixed Lymphocyte Culture ◽  
Lymphocyte Culture ◽  
Red Cell ◽  
Curative Therapy ◽  
Red Cell Aplasia ◽  
First Year Of Life

Abstract Constitutional pure red cell aplasia (CPRCA) is a syndrome of failed erythropoiesis usually diagnosed within the first year of life. Four patients with CPRCA received transplants with marrow from their HLA- identical, mixed lymphocyte culture-nonreactive siblings. All patients were resistant to corticosteroid therapy and were dependent on regular red cell transfusions for at least 5 years. Three patients were conditioned with procarbazine, antithymocyte globulin, cyclophosphamide, and busulfan, and one was conditioned with antithymocyte serum, cyclophosphamide, and busulfan. Three patients promptly had successful engraftments with establishment of donor hematopoiesis. One patient initially rejected his graft but received a successful retransplant. All patients are currently alive with Karnofsky performance scores of 100 and normal erythropoiesis of donor origin. Despite a history of multiple transfusions, bone marrow transplantation is a potentially curative therapy for patients with CPRCA.

scholarly journals Bone marrow transplantation for constitutional pure red cell aplasia

Blood ◽  
1988 ◽  
Vol 71 (1) ◽  
pp. 226-229
Author(s):  
C Lenarsky ◽  
K Weinberg ◽  
E Guinan ◽  
PP Dukes ◽  
Y Barak ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Marrow Transplantation ◽  
Pure Red Cell Aplasia ◽  
Mixed Lymphocyte Culture ◽  
Lymphocyte Culture ◽  
Red Cell ◽  
Curative Therapy ◽  
Red Cell Aplasia ◽  
First Year Of Life

Constitutional pure red cell aplasia (CPRCA) is a syndrome of failed erythropoiesis usually diagnosed within the first year of life. Four patients with CPRCA received transplants with marrow from their HLA- identical, mixed lymphocyte culture-nonreactive siblings. All patients were resistant to corticosteroid therapy and were dependent on regular red cell transfusions for at least 5 years. Three patients were conditioned with procarbazine, antithymocyte globulin, cyclophosphamide, and busulfan, and one was conditioned with antithymocyte serum, cyclophosphamide, and busulfan. Three patients promptly had successful engraftments with establishment of donor hematopoiesis. One patient initially rejected his graft but received a successful retransplant. All patients are currently alive with Karnofsky performance scores of 100 and normal erythropoiesis of donor origin. Despite a history of multiple transfusions, bone marrow transplantation is a potentially curative therapy for patients with CPRCA.

scholarly journals Successful bone marrow transplantation against mixed lymphocyte culture barrier

Blood ◽  
1976 ◽  
Vol 48 (3) ◽  
pp. 385-391 ◽  
Author(s):  
SA Feig ◽  
G Opelz ◽  
HS Winter ◽  
PM Falk ◽  
RC Neerhout ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Aplastic Anemia ◽  
Genetic Markers ◽  
Marrow Transplantation ◽  
Mixed Lymphocyte Culture ◽  
Lymphocyte Culture ◽  
Host Disease ◽  
The Face

Abstract If bone marrow transplantation is to become widely applicable in the treatment of patients with leukemia and aplastic anemia, the necessity to have a perfectly histocompatible donor must be overcome. In an effort to define the roles of HL-A type and mixed lymphocyte culture (MLC) reactivity in the determination of successful engraftment and the occurrence of graftversus-host disease (GVHD), we have attempted transplantation of a child with acute myeloblastic leukemia (AML) using an HL-A identical, MLC-reactive sibling donor. Successful engraftment has been accomplished, as documented by the appearance of multiple donor genetic markers in the recipient. There is no evidence of severe GVHD. The recipient is alive, without evidence of leukemia, and has returned to full activities 9 mo after transplantation. The recipient now produces lymphocytes, which have the MLC reactivity that characterize the donor's lymphocytes, rather than that of her own pretransplant lymphocytes. This experience demonstrates that successful bone marrow transplantation in patients with leukemia can be accomplished in the face of MLC reactivity.

Sign in / Sign up

Export Citation Format

Share Document