EFFECT OF SERUM ON HUMAN BONE MARROW STROMAL CELLS: EX VIVO EXPANSION AND IN VIVO BONE FORMATION

2000 ◽  
Vol 70 (12) ◽  
pp. 1780-1787 ◽  
Author(s):  
Sergei A. Kuznetsov ◽  
Mahesh H. Mankani ◽  
Pamela Gehron Robey
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Ex Vivo ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
In Vivo Bone Formation

scholarly journals Association between in vivo bone formation and ex vivo migratory capacity of human bone marrow stromal cells

2015 ◽  
Vol 6 (1) ◽  
Author(s):  
Rikke K. Andersen ◽  
Walid Zaher ◽  
Kenneth H. Larsen ◽  
Nicholas Ditzel ◽  
Katharina Drews ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Ex Vivo ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Migratory Capacity ◽  
In Vivo Bone Formation

In Vivo Bone Formation by Human Bone Marrow Stromal Cells: Reconstruction of the Mouse Calvarium and Mandible

Stem Cells ◽  
2006 ◽  
Vol 24 (9) ◽  
pp. 2140-2149 ◽  
Author(s):  
Mahesh H. Mankani ◽  
Sergei A. Kuznetsov ◽  
Raymond M. Wolfe ◽  
Grayson W. Marshall ◽  
Pamela Gehron Robey
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
In Vivo Bone Formation

In vivo bone formation by human bone marrow stromal cells: Effect of carrier particle size and shape

2000 ◽  
Vol 72 (1) ◽  
pp. 96-107 ◽  
Author(s):  
Mahesh H. Mankani ◽  
Sergei A. Kuznetsov ◽  
Bruce Fowler ◽  
Albert Kingman ◽  
Pamela Gehron Robey
Keyword(s):  
Bone Marrow ◽  
Particle Size ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Particle Size And Shape ◽  
In Vivo Bone Formation

Effectiveness of Cryo-Preserved Human Bone Marrow Stromal Cells at Forming Bone in Vivo

2005 ◽  
Vol 116 (Supplement) ◽  
pp. 150-151
Author(s):  
Mahesh H. Mankani ◽  
Sergei Kuznetsov ◽  
Pamela Gehron Robey
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow

scholarly journals An in vivo model to assess magnesium alloys and their biological effect on human bone marrow stromal cells

2015 ◽  
Vol 28 ◽  
pp. 234-239 ◽  
Author(s):  
Sayuri Yoshizawa ◽  
Amy Chaya ◽  
Kostas Verdelis ◽  
Elizabeth A. Bilodeau ◽  
Charles Sfeir
Keyword(s):  
Bone Marrow ◽  
Magnesium Alloys ◽  
Stromal Cells ◽  
Biological Effect ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
In Vivo Model

scholarly journals Lamellar Spacing in Cuboid Hydroxyapatite Scaffolds Regulates Bone Formation by Human Bone Marrow Stromal Cells

2011 ◽  
Vol 17 (11-12) ◽  
pp. 1615-1623 ◽  
Author(s):  
Mahesh H. Mankani ◽  
Shahrzad Afghani ◽  
Jaime Franco ◽  
Max Launey ◽  
Sally Marshall ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Lamellar Spacing ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow

Aged Human Bone Marrow Stromal Cells Maintaining Bone Forming Capacity in vivo Evaluated Using an Improved Method of Visualization

2004 ◽  
Vol 5 (2) ◽  
pp. 107-118 ◽  
Author(s):  
K. Stenderup ◽  
C. Rosada ◽  
J. Justesen ◽  
T. Al-Soubky ◽  
F. Dagnaes-Hansen ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Improved Method

Relation between in vitro and in vivo osteogenic potential of cultured human bone marrow stromal cells

2004 ◽  
Vol 15 (10) ◽  
pp. 1123-1128 ◽  
Author(s):  
S. C. Mendes ◽  
J. M. Tibbe ◽  
M. Veenhof ◽  
S. Both ◽  
F. C. Oner ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteogenic Potential

scholarly journals Effects of dexamethasone on proliferation, activity, and cytokine secretion of normal human bone marrow stromal cells: possible mechanisms of glucocorticoid-induced bone loss

1999 ◽  
Vol 162 (3) ◽  
pp. 371-379 ◽  
Author(s):  
CH Kim ◽  
SL Cheng ◽  
GS Kim
Keyword(s):  
Bone Marrow ◽  
Bone Resorption ◽  
Bone Loss ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Cytokine Secretion ◽  
Human Bone Marrow

It is well documented that glucocorticoid excess causes bone loss, but the mechanisms of these effects remain poorly defined. To understand further the mechanisms of glucocorticoid-induced osteoporosis, we investigated the effects of glucocorticoids on bone formation and bone resorption by examining the proliferation, functional activities, and cytokine secretion of cultured human bone marrow stromal cells (hBMSC). Treatment with dexamethasone for 24 h at the concentration of 10(-8) M significantly suppressed [(3)H]thymidine incorporation and further inhibition was observed with longer treatment (8 days) or higher concentration (10(-7) M). Alkaline phosphatase activity of hBMSC was markedly stimulated with addition of dexamethasone (10(-8) M), to 191 +/- 22% (after 4 days) and 317 +/- 46% (after 7 days) of control. Dexamethasone (10(-8) M) treatment for 48 h decreased the incorporation of [(3)H]proline into collagenase-digestible protein (CDP; 43.7+/-7.9% of control) and non-collagen protein (65.2+/-8.4% of control), with a greater effect on CDP. Northern blot analysis indicated that alpha1(I)-collagen mRNA level was decreased by dexamethasone to 27.6 +/- 9.0% of the control value after 1 day of exposure, and to 55.2 +/- 6.2% after 7 days. Dexamethasone markedly suppressed basal production of interleukin (IL)-6 and IL-11 and that stimulated by parathyroid hormone (PTH), IL-1alpha, or tumour necrosis factor-alpha in a dose-dependent manner. These results suggest that the glucocorticoid-induced bone loss is derived at least in part via inhibition of bone formation, which includes the suppression of osteoblast proliferation and collagen synthesis. As both basal and PTH-stimulated production of IL-6 and IL-11 are decreased by dexamethasone, the increased bone resorption observed in glucocorticoid-induced osteopenia does not appear to be mediated by IL-6 or IL-11.

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