Deep Anterior Lamellar Keratoplasty With Melles Technique for Granular Corneal Dystrophy

Cornea ◽  
2009 ◽  
Vol 28 (2) ◽  
pp. 140-143 ◽  
Author(s):  
Ramin Salouti ◽  
Hamid Hosseini ◽  
Masoomeh Eghtedari ◽  
Mohammad R Khalili
Keyword(s):  
Corneal Dystrophy ◽  
Lamellar Keratoplasty ◽  
Deep Anterior Lamellar Keratoplasty ◽  
Anterior Lamellar Keratoplasty ◽  
Granular Corneal Dystrophy

scholarly journals Compound Heterozygous Mutations in TGFBI Cause a Severe Phenotype of Granular Corneal Dystrophy Type 2

2021 ◽  
Author(s):  
Ikhyun Jun ◽  
Yong Woo Ji ◽  
Seung-il Choi ◽  
Bo Ram Lee ◽  
Ji Sang Min ◽  
...  
Keyword(s):  
Corneal Dystrophy ◽  
Lamellar Keratoplasty ◽  
Deep Anterior Lamellar Keratoplasty ◽  
Compound Heterozygosity ◽  
Slit Lamp ◽  
Severe Phenotype ◽  
Corneal Dystrophies ◽  
Anterior Lamellar Keratoplasty ◽  
Granular Corneal Dystrophy

Abstract We investigated the clinical and genetic features of patients with severe phenotype of granular corneal dystrophy type 2 (GCD2) associated with compound heterozygosity in the transforming growth factor-β-induced (TGFBI) gene. Patients with severe GCD2 underwent ophthalmic examination (best-corrected visual acuity test, intraocular pressure measurement, slit-lamp examination, and slit-lamp photograph analysis) and direct Sanger sequencing of whole-TGFBI. The patient’s family was tested to determine the pedigrees. Five novel mutations (p.His174Asp, p.Ile247Asn, p.Tyr88Cys, p.Arg257Pro, and p.Tyr468*) and two known mutations (p.Asn544Ser and p.Arg179*) in TGFBI were identified, along with p.Arg124His, in the patients. Trans-phase of TGFBI second mutations was confirmed by pedigree analysis. Multiple, extensive discoid granular, and increased linear deposits were observed in the probands carrying p.Arg124His and other nonsense mutations. Some patients who had undergone phototherapeutic keratectomy experienced rapid recurrence (p.Ile247Asn and p.Asn544Ser); however, the cornea was well-maintained in a patient who underwent deep anterior lamellar keratoplasty (p.Ile247Asn). Thus, compound heterozygosity of TGFBI is associated with the phenotypic variability of TGFBI corneal dystrophies, suggesting that identifying TGFBI second mutations may be vital in patients with extraordinarily severe phenotypes. Our findings indicate the necessity for a more precise observation of genotype-phenotype correlation and additional care when treating TGFBI corneal dystrophies.

scholarly journals Compound heterozygous mutations in TGFBI cause a severe phenotype of granular corneal dystrophy type 2

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ikhyun Jun ◽  
Yong Woo Ji ◽  
Seung-il Choi ◽  
Bo Ram Lee ◽  
Ji Sang Min ◽  
...  
Keyword(s):  
Corneal Dystrophy ◽  
Lamellar Keratoplasty ◽  
Deep Anterior Lamellar Keratoplasty ◽  
Compound Heterozygosity ◽  
Slit Lamp ◽  
Severe Phenotype ◽  
Corneal Dystrophies ◽  
Anterior Lamellar Keratoplasty ◽  
Granular Corneal Dystrophy

AbstractWe investigated the clinical and genetic features of patients with severe phenotype of granular corneal dystrophy type 2 (GCD2) associated with compound heterozygosity in the transforming growth factor-β-induced (TGFBI) gene. Patients with severe GCD2 underwent ophthalmic examination (best-corrected visual acuity test, intraocular pressure measurement, slit-lamp examination, and slit-lamp photograph analysis) and direct Sanger sequencing of whole-TGFBI. The patient’s family was tested to determine the pedigrees. Five novel mutations (p.(His174Asp), p.(Ile247Asn), p.(Tyr88Cys), p.(Arg257Pro), and p.(Tyr468*)) and two known mutations (p.(Asn544Ser) and p.(Arg179*)) in TGFBI were identified, along with p.(Arg124His), in the patients. Trans-phase of TGFBI second mutations was confirmed by pedigree analysis. Multiple, extensive discoid granular, and increased linear deposits were observed in the probands carrying p.(Arg124His) and other nonsense mutations. Some patients who had undergone phototherapeutic keratectomy experienced rapid recurrence (p.(Ile247Asn) and p.(Asn544Ser)); however, the cornea was well-maintained in a patient who underwent deep anterior lamellar keratoplasty (p.(Ile247Asn)). Thus, compound heterozygosity of TGFBI is associated with the phenotypic variability of TGFBI corneal dystrophies, suggesting that identifying TGFBI second mutations may be vital in patients with extraordinarily severe phenotypes. Our findings indicate the necessity for a more precise observation of genotype–phenotype correlation and additional care when treating TGFBI corneal dystrophies.

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