scholarly journals Magnetic resonance spectroscopy of isocitrate dehydrogenase mutated gliomas: current knowledge on the neurochemical profile

2020 ◽  
Vol 33 (4) ◽  
pp. 413-421 ◽  
Author(s):  
Francesca Branzoli ◽  
Małgorzata Marjańska
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Isocitrate Dehydrogenase ◽  
Current Knowledge ◽  
Resonance Spectroscopy ◽  
Neurochemical Profile

scholarly journals Sperm Metabolomics through Nuclear Magnetic Resonance Spectroscopy

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1669
Author(s):  
Marta Lombó ◽  
Sara Ruiz-Díaz ◽  
Alfonso Gutiérrez-Adán ◽  
María-Jesús Sánchez-Calabuig
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Nuclear Magnetic Resonance Spectroscopy ◽  
Current Knowledge ◽  
Proton Nuclear Magnetic Resonance ◽  
Farm Animals ◽  
Plasma Metabolites ◽  
Resonance Spectroscopy ◽  
Nuclear Magnetic

This report reviews current knowledge of sperm metabolomics analysis using proton nuclear magnetic resonance spectroscopy (1 H-NMR) with particular emphasis on human and farm animals. First, we present the benefits of NMR over other techniques to identify sperm metabolites and then describe the specific methodology required for NMR sperm analysis, stressing the importance of analyzing metabolites extracted from both the hydrophilic and lipophilic phases. This is followed by a description of advances produced to date in the use of NMR to diagnose infertility in humans and to identify metabolic differences among the sperm of mammalian herbivore, carnivore, and omnivore species. This last application of NMR mainly seeks to explore the possible use of lipids to fuel sperm physiology, contrary to previous theories that glycolysis and oxidative phosphorylation (OXPHOS) are the only sources of sperm energy. This review describes the use of NMR to identify sperm and seminal plasma metabolites as possible indicators of semen quality, and to examine the metabolites needed to maintain sperm motility, induce their capacitation, and consequently, to predict animal fertility.

scholarly journals Metabolic signature in nucleus accumbens for anti-depressant-like effects of acetyl-L-carnitine: Anin vivo1H-magnetic resonance spectroscopy study at 14 T

2019 ◽  
Author(s):  
Antoine Cherix ◽  
Thomas Larrieu ◽  
Jocelyn Grosse ◽  
João Rodrigues ◽  
Bruce McEwen ◽  
...  
Keyword(s):  
Nucleus Accumbens ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Social Rank ◽  
Resonance Spectroscopy ◽  
Magnetic Resonance Spectroscopy Study ◽  
Metabolic Signature ◽  
Neurochemical Profile ◽  
Hierarchical Status

AbstractBackgroundEmerging evidence suggests that hierarchical status may provide vulnerability to develop stress-induced depression. Energy metabolism in the nucleus accumbens (NAc) was recently related to hierarchical status and vulnerability to develop depression-like behavior. Acetyl-L-carnitine (LAC), a mitochondria-boosting supplement, has shown promising antidepressant-like effects opening promising therapeutic strategies for restoring energy balance in depressed patients. Here, we investigated the metabolic impact in the NAc of antidepressant LAC treatment in chronically stressed mice.MethodMice were characterized for emotional behaviors and social rank. They were then exposed to chronic restraint stress (CRS) for 21 days and subsequently tested in a social behavior (SB) test. A group of mice was also given LAC supplementation during the 7 last CRS days. Mice were then tested in the SB and forced swim tests (FST) and scannedin vivousing1H-magnetic resonance spectroscopy (1H-MRS) to quantitatively assess the NAc neurochemical profile.ResultsDominant, but not subordinate, mice showed behavioral vulnerability to CRS. In the NAc, dominant mice showed reduced levels of several energy-related metabolites. LAC treatment counteracted stress-induced behavioral changes in dominant mice, and normalized levels of taurine, phosphocreatine, glutamine and phosphocholine in the NAc. No major accumbal metabolic changes were observed in subordinate mice.ConclusionHigh social rank is confirmed as a vulnerability factor to develop chronic stress-induced depressive-like behaviors. We reveal a metabolic signature in the NAc for the antidepressant-like effects of LAC in vulnerable mice, characterized by restoration of stress-induced alterations in neuroenergetics and lipid function.

scholarly journals Noninvasive assessment of isocitrate dehydrogenase mutation status in cerebral gliomas by magnetic resonance spectroscopy in a clinical setting

2018 ◽  
Vol 128 (2) ◽  
pp. 391-398 ◽  
Author(s):  
Anna Tietze ◽  
Changho Choi ◽  
Bruce Mickey ◽  
Elizabeth A. Maher ◽  
Benedicte Parm Ulhøi ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Isocitrate Dehydrogenase ◽  
Prognostic Significance ◽  
Resonance Spectroscopy ◽  
Idh Mutation ◽  
Imaging Protocol ◽  
Mutation Status ◽  
Idh Mutations ◽  
Cerebral Gliomas

OBJECTIVEMutations in the isocitrate dehydrogenase (IDH) genes are of proven diagnostic and prognostic significance for cerebral gliomas. The objective of this study was to evaluate the clinical feasibility of using a recently described method for determining IDH mutation status by using magnetic resonance spectroscopy (MRS) to detect the presence of 2-hydroxyglutarate (2HG), the metabolic product of the mutant IDH enzyme.METHODSBy extending imaging time by 6 minutes, the authors were able to include a point-resolved spectroscopy (PRESS) MRS sequence in their routine glioma imaging protocol. In 30 of 35 patients for whom this revised protocol was used the lesions were subsequently diagnosed histologically as gliomas. Of the remaining 5 patients, 1 had a gangliocytoma, 1 had a primary CNS lymphoma, and 3 had nonneoplastic lesions. Immunohistochemistry and/or polymerase chain reaction were used to detect the presence of IDH mutations in the glioma tissue resected.RESULTSIn vivo MRS for 2HG correctly identified the IDH mutational status in 88.6% of patients. The sensitivity and specificity was 89.5% and 81.3%, respectively, when using 2 mM 2HG as threshold to discriminate IDH-mutated from wildtype tumors. Two glioblastomas that had elevated 2HG levels did not have detectable IDH mutations, and in 2 IDH-mutated gliomas 2HG was not reliably detectable.CONCLUSIONSThe noninvasive determination of the IDH mutation status of a presumed glioma by means of MRS may be incorporated into a routine diagnostic imaging protocol and can be used to obtain additional information for patient care.

scholarly journals Expanded neurochemical profile in the early stage of Huntington disease using proton magnetic resonance spectroscopy

2018 ◽  
Vol 31 (3) ◽  
pp. e3880 ◽  
Author(s):  
Isaac M. Adanyeguh ◽  
Marie-Lorraine Monin ◽  
Daisy Rinaldi ◽  
Léorah Freeman ◽  
Alexandra Durr ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Huntington Disease ◽  
Proton Magnetic Resonance ◽  
Proton Magnetic Resonance Spectroscopy ◽  
Early Stage ◽  
Resonance Spectroscopy ◽  
Neurochemical Profile

scholarly journals Hippocampal Neurochemical Profile and Glucose Transport Kinetics in Patients With Type 1 Diabetes

2019 ◽  
Vol 105 (2) ◽  
pp. 479-491
Author(s):  
Petr Bednařík ◽  
Pierre-Gilles Henry ◽  
Amir Khowaja ◽  
Nathan Rubin ◽  
Anjali Kumar ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Magnetic Resonance ◽  
Magnetic Resonance Spectroscopy ◽  
Glucose Transport ◽  
Medical Center ◽  
Resonance Spectroscopy ◽  
Single Voxel ◽  
Impaired Awareness ◽  
Neurochemical Profile

Abstract Context Longstanding type 1 diabetes (T1D) may lead to alterations in hippocampal neurochemical profile. Upregulation of hippocampal glucose transport as a result of recurrent exposure to hypoglycemia may preserve cognitive function during future hypoglycemia in subjects with T1D and impaired awareness of hypoglycemia (IAH). The effect of T1D on hippocampal neurochemical profile and glucose transport is unknown. Objective To test the hypothesis that hippocampal neurochemical composition is altered in T1D and glucose transport is upregulated in T1D with IAH. Design and participants Hippocampal neurochemical profile was measured with single-voxel magnetic resonance spectroscopy at 3T during euglycemia in 18 healthy controls (HC), 10 T1D with IAH, and 12 T1D with normal awareness to hypoglycemia (NAH). Additionally, 12 HC, 8 T1D-IAH, and 6 T1D-NAH were scanned during hyperglycemia to assess hippocampal glucose transport with metabolic modeling. Setting University medical center. Main Outcome Measures Concentrations of hippocampal neurochemicals measured during euglycemia and ratios of maximal transport rate to cerebral metabolic rate of glucose (Tmax/CMRGlc), derived from magnetic resonance spectroscopy–measured hippocampal glucose as a function of plasma glucose. Results Comparison of hippocampal neurochemical profile revealed no group differences (HC, T1D, T1D-IAH, and T1D-NAH). The ratio Tmax/CMRGlc was not significantly different between the groups, T1D-IAH (1.58 ± 0.09) and HC (1.65 ± 0.07, P = 0.54), between T1D-NAH (1.50 ± 0.09) and HC (P = 0.19), and between T1D-IAH and T1D-NAH (P = 0.53). Conclusions Subjects with T1D with sufficient exposure to recurrent hypoglycemia to create IAH did not have alteration of Tmax/CMRglc or neurochemical profile compared with participants with T1D-NAH or HC.

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