scholarly journals Cloning and Sequencing of 5' Terminal Sequences from Avian Infectious Bronchitis Virus Genomic RNA

1986 ◽  
Vol 67 (2) ◽  
pp. 221-228 ◽  
Author(s):  
T. D. K. Brown ◽  
M. E. G. Boursnell ◽  
M. M. Binns ◽  
F. M. Tomley
Virology ◽  
1988 ◽  
Vol 165 (2) ◽  
pp. 589-595 ◽  
Author(s):  
Shizuyo Sutou ◽  
Seiji Sato ◽  
Tatsuji Okabe ◽  
Masahisa Nakai ◽  
Norimasa Sasaki

1998 ◽  
Vol 26 (5) ◽  
pp. 629-634
Author(s):  
Emiliana Falcone ◽  
Edoardo Vignolo ◽  
Livia Di Trani ◽  
Simona Puzelli ◽  
Maria Tollis

A reverse transcriptase polymerase chain reaction (RT-PCR) assay specific for identifying avian infectious bronchitis virus (IBV) in poultry vaccines, and the serological response to IBV induced by the inoculation of chicks with a Newcastle disease vaccine spiked with the Massachusetts strain of IBV, were compared for their ability to detect IBV as a contaminant of avian vaccines. The sensitivity of the IBV-RT-PCR assay provided results which were at least equivalent to the biological effect produced by the inoculation of chicks, allowing this assay to be considered a valid alternative to animal testing in the quality control of avian immunologicals. This procedure can easily be adapted to detect a number of contaminants for which the in vivo test still represents the only available method of detection.


2000 ◽  
Vol 146 (7) ◽  
pp. 191-193 ◽  
Author(s):  
A. Zanella ◽  
R. Coaro ◽  
R. Marchi ◽  
G. Fabris ◽  
A. Lavazza

1987 ◽  
Vol 66 (4) ◽  
pp. 765-767 ◽  
Author(s):  
MOHAMMAD A. MUNEER ◽  
JOHN A. NEWMAN ◽  
SAGAR M. GOYAL ◽  
M. AJMAL

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