scholarly journals Partial Purification and Some Properties of ATP: Citrate Lyase from the Oleaginous Yeast Lipomyces starkeyi

Microbiology ◽  
1983 ◽  
Vol 129 (9) ◽  
pp. 2863-2869 ◽  
Author(s):  
C. A. Boulton ◽  
C. Ratledge
Keyword(s):  
Oleaginous Yeast ◽  
Partial Purification ◽  
Lipomyces Starkeyi ◽  
Atp Citrate Lyase ◽  
Citrate Lyase

scholarly journals Citrate-Mediated Acyl-CoA Synthesis Is Required for the Promotion of Growth and Triacylglycerol Production in Oleaginous Yeast Lipomyces starkeyi

2021 ◽  
Vol 9 (8) ◽  
pp. 1693
Author(s):  
Rikako Sato ◽  
Satoshi Ara ◽  
Harutake Yamazaki ◽  
Koji Ishiya ◽  
Sachiyo Aburatani ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Gradient Centrifugation ◽  
Screening Method ◽  
Lipid Productivity ◽  
Biodiesel Production ◽  
Lipomyces Starkeyi ◽  
Atp Citrate Lyase ◽  
Percoll Density Gradient Centrifugation ◽  
Citrate Lyase ◽  
Percoll Density Gradient

The oleaginous yeast Lipomyces starkeyi is an excellent producer of triacylglycerol (TAG) as a feedstock for biodiesel production. To understand the regulation of TAG synthesis, we attempted to isolate mutants with decreased lipid productivity and analyze the expression of TAG synthesis-related genes in this study. A mutant with greatly decreased lipid productivity, sr22, was obtained by an effective screening method using Percoll density gradient centrifugation. The expression of citrate-mediated acyl-CoA synthesis-related genes (ACL1, ACL2, ACC1, FAS1, and FAS2) was decreased in the sr22 mutant compared with that of the wild-type strain. Together with a notion that L. starkeyi mutants with increased lipid productivities had increased gene expression, there was a correlation between the expression of these genes and TAG synthesis. To clarify the importance of citrate-mediated acyl-CoA synthesis pathway on TAG synthesis, we also constructed a strain with no ATP-citrate lyase responsible for the first reaction of citrate-mediated acyl-CoA synthesis and investigated the importance of ATP-citrate lyase on TAG synthesis. The ATP-citrate lyase was required for the promotion of cell growth and TAG synthesis in a glucose medium. This study may provide opportunities for the development of an efficient TAG synthesis for biodiesel production.

Cloning and Expression Analysis of ATP-Citrate Lyase Genes from Sugarcane

2013 ◽  
Vol 38 (11) ◽  
pp. 2024-2033 ◽  
Author(s):  
Chang-Ning LI ◽  
Qian NONG ◽  
Qin-Liang TAN ◽  
SRIVASTAVA Manoj Kumar ◽  
Li-Tao YANG ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Cloning And Expression ◽  
Atp Citrate Lyase ◽  
Citrate Lyase

Genetically Engineered Oleaginous Yeast Lipomyces starkeyi for Sesquiterpene α-Zingiberene Production

2021 ◽  
Author(s):  
Ziyu Dai ◽  
Kyle R. Pomraning ◽  
Ellen A. Panisko ◽  
Beth A. Hofstad ◽  
Kristen B. Campbell ◽  
...  
Keyword(s):  
Oleaginous Yeast ◽  
Genetically Engineered ◽  
Lipomyces Starkeyi

Acetyl-CoA is produced by the citrate synthase homology module of ATP-citrate lyase

2021 ◽  
Author(s):  
Kenneth Verstraete ◽  
Koen H. G. Verschueren ◽  
Ann Dansercoer ◽  
Savvas N. Savvides
Keyword(s):  
Citrate Synthase ◽  
Atp Citrate Lyase ◽  
Acetyl Coa ◽  
Citrate Lyase ◽  
Homology Module

scholarly journals IGF1-mediated HOXA13 overexpression promotes colorectal cancer metastasis through upregulating ACLY and IGF1R

2021 ◽  
Vol 12 (6) ◽  
Author(s):  
Chenyang Qiao ◽  
Wenjie Huang ◽  
Jie Chen ◽  
Weibo Feng ◽  
Tongyue Zhang ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Growth Factor ◽  
Cancer Metastasis ◽  
Combined Treatment ◽  
Insulin Like Growth Factor ◽  
Atp Citrate Lyase ◽  
Citrate Lyase ◽  
Elevated Expression ◽  
Igf1r Inhibitor ◽  
Colorectal Cancer Metastasis

AbstractMetastasis is the major reason for the high mortality of colorectal cancer (CRC) patients and its molecular mechanism remains unclear. Here, we report a novel role of Homeobox A13 (HOXA13), a member of the Homeobox (HOX) family, in promoting CRC metastasis. The elevated expression of HOXA13 was positively correlated with distant metastasis, higher AJCC stage, and poor prognosis in two independent CRC cohorts. Overexpression of HOXA13 promoted CRC metastasis whereas downregulation of HOXA13 suppressed CRC metastasis. Mechanistically, HOXA13 facilitated CRC metastasis by transactivating ATP-citrate lyase (ACLY) and insulin-like growth factor 1 receptor (IGF1R). Knockdown of ACLY and IGFIR inhibited HOXA13-medicated CRC metastasis, whereas ectopic overexpression of ACLY and IGFIR rescued the decreased CRC metastasis induced by HOXA13 knockdown. Furthermore, Insulin-like growth factor 1 (IGF1), the ligand of IGF1R, upregulated HOXA13 expression through the PI3K/AKT/HIF1α pathway. Knockdown of HOXA13 decreased IGF1-mediated CRC metastasis. In addition, the combined treatment of ACLY inhibitor ETC-1002 and IGF1R inhibitor Linsitinib dramatically suppressed HOXA13-mediated CRC metastasis. In conclusion, HOXA13 is a prognostic biomarker in CRC patients. Targeting the IGF1-HOXA13-IGF1R positive feedback loop may provide a potential therapeutic strategy for the treatment of HOXA13-driven CRC metastasis.

scholarly journals Purification of a hepatic 123,000-dalton hormone-stimulated 32P-peptide and its identification as ATP-citrate lyase.

1979 ◽  
Vol 254 (16) ◽  
pp. 8052-8056 ◽  
Author(s):  
M.C. Alexander ◽  
E.M. Kowaloff ◽  
L.A. Witters ◽  
D.T. Dennihy ◽  
J. Avruch
Keyword(s):  
Atp Citrate Lyase ◽  
Citrate Lyase
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