scholarly journals Blastobotrys baotianmanensis sp. nov. and Blastobotrys xishuangbannaensis f.a., sp. nov., two novel yeast species associated with insects and rotting wood

2020 ◽  
Vol 70 (7) ◽  
pp. 4217-4223
Author(s):  
Chun-Yue Chai ◽  
Ran-Ran Jia ◽  
Chong-Yi Chen ◽  
Feng-Li Hui
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Rrna Gene ◽  
Undescribed Species ◽  
Yeast Strains ◽  
Rotting Wood ◽  
Lsu Rrna Gene ◽  
Lsu Rrna ◽  
Type Strains

Five yeast strains were isolated from the gut of the groundbeetle Pterostichus gebleri and rotting wood, which were collected from two different localities in China. These strains were identified as representing two novel species of the genus Blastobotrys through comparison of sequences in the D1/D2 domains of the LSU rRNA gene and other taxonomic characteristics. Blastobotrys baotianmanensis sp. nov. produces two to three spherical ascospores per ascus, and is most closely related to the type strains of B. elegans, B. capitulata, B. arbuscula, and an undescribed species represented by strain BG02-7-20-006A-3-1. Blastobotrys baotianmanensis sp. nov. differed from these strains by 3.6–8.4 % divergence (21–46 substitutions and 0–4 gaps) in the D1/D2 sequences. Blastobotrys xishuangbannaensis f.a., sp. nov. is closely related to B. nivea, B. elegans and B. aristata but the formation of ascospores was not observed on various sporulation media, and it differed from its relatives by 6.2–8.5 % divergence (34–43 substitutions and 2–6 gaps) in the D1/D2 sequences. The holotype of Blastobotrys baotianmanensis sp. nov. is NYNU 1581 and the holotype of Blastobotrys xishuangbannaensis f.a., sp. nov. is NYNU 181030.

scholarly journals Teunomyces basidiocarpi sp. nov. and Teunomyces luguensis sp. nov., two ascomycetous yeast species related to the Candida kruisii clade isolated in Taiwan

2020 ◽  
Vol 70 (3) ◽  
pp. 2103-2107
Author(s):  
Chin-Feng Chang ◽  
Yi-Ru Liu ◽  
Ching-Fu Lee
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Rrna Gene ◽  
Ascomycetous Yeast ◽  
Domain Sequence ◽  
D2 Domain ◽  
Lsu Rrna Gene ◽  
Lsu Rrna ◽  
Physiological Tests ◽  
Type Strains

Four strains of anamorphic yeasts isolated from the fruiting bodies of mushrooms collected in Taiwan were found to represent two novel yeast species belonging to the genus Teunomyces, which was formally known as the Candida kruisii clade. Strains NY13M09T and NY14M14 were related to the type strains of Teunomyces panamensis, T. pallodes, T. tritomae and T. lycoperdinae, and strains GG4M07T and GG6M14 were related to T. kruisii NRRL Y-17087T and T. cretensis NRRL Y-27777T. However, strains NY13M09T and NY14M14 differed from their closest phylogenetic neighbours by 2.9–3.7 % in the D1/D2 domain sequence of the LSU rRNA gene and by 6.6–13.7 % in the internal transcribed spacer (ITS); GG4M07T and GG6M14 differed from their closest known species by 2.4 % in the D1/D2 domain sequence of the LSU rRNA gene and by 8.7–10.0 % in the ITS. Meanwhile, these strains were also clearly distinguished from their closest relatives based on the results of physiological tests. Based on the characteristics described above, the strains could be regarded as representing two novel species of the genus Teunomyces, for which the names Teunomyces basidiocarpi sp. nov. and Teunomyces luguensis sp. nov. are proposed. The holotypes are Teunomyces basidiocarpi BCRC 23475T and Teunomyces luguensis BCRC 23476T.

scholarly journals Candida baotianmanensis sp. nov. and Candida pseudoviswanathii sp. nov., two ascosporic yeast species isolated from the gut of beetles

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3580-3585 ◽  
Author(s):  
Yong-Cheng Ren ◽  
Long-Long Xu ◽  
Lin Zhang ◽  
Feng-Li Hui
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Phenotypic Traits ◽  
Rrna Gene ◽  
Candida Maltosa ◽  
Yeast Strains ◽  
Lsu Rrna Gene ◽  
Candida Viswanathii ◽  
Lsu Rrna

Four yeast strains were isolated from the gut of beetles collected on Baotianman Mountain and People's Park of Nanyang in Henan Province, China. These strains produced unconjugated asci with one or two ellipsoidal to elongate ascospores in a persistent ascus. Phylogenetic analysis of the D1/D2 domains of the LSU rRNA gene sequences indicated that the isolates represent two novel sexual species in the Candida/Lodderomyces clade. Candida baotianmanensis sp. nov. was located in a statistically well-supported branch together with Candida maltosa. Candida pseudoviswanathii sp. nov. formed a subclade with its closest relative Candida viswanathii supported by a strong bootstrap value. The two novel species were distinguished from their most closely related described species, Candida maltosa and Candida viswanathii, in the D1/D2 LSU rRNA gene and internal transcribed spacer (ITS) sequences and in phenotypic traits. The type strain of Candida baotianmanensis sp. nov. is NYNU 14719T ( = CBS 13915T = CICC 33052T), and the type strain of Candida pseudoviswanathii sp. nov. is NYNU 14772T ( = CBS 13916T = CICC 33053T). The MycoBank numbers for Candida baotianmanensis sp. nov. and Candida pseudoviswanathii sp. nov. are MB 812621 and MB 812622.

Citeromyces nyonsensis sp. nov., a novel yeast species isolated from black olive brine

2013 ◽  
Vol 63 (Pt_8) ◽  
pp. 3086-3090 ◽  
Author(s):  
Serge Casaregola ◽  
Noémie Jacques ◽  
Christelle Louis-Mondesir ◽  
Monika Coton ◽  
Emmanuel Coton
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Gene ◽  
Sequence Analyses ◽  
Lsu Rrna Gene ◽  
Black Olive ◽  
Lsu Rrna ◽  
The Town

A yeast strain was isolated from olive brines in a fermented black olive and olive oil manufacturing plant in the town of Nyons (France). On the basis of domains 1 and 2 (D1/D2) large subunit (LSU) rRNA gene and internal transcribed spacer (ITS) region sequence analyses, the strain CLIB 1303T was found to be closely related, but clearly distinct, from the three existing species of the genus Citeromyces: Citeromyces matritensis, Citeromyces siamensis and Citeromyces haiwaiiensis. Strain CLIB 1303T exhibited 6 bp, 7 bp and 12 bp divergences in the D1/D2 LSU rRNA gene with C. siamensis, C. matritensis and C. hawaiiensis, respectively. ITS region divergence amounted to more than 8 %, 4 % and 4.5 % with C. siamensis, C. matritensis and C. hawaiiensis, respectively, in addition to several indels. Like C. matritensis and C. siamensis strains, strain CLIB 1303T was shown to be halotolerant and osmotolerant. Phenotypically, strain CLIB 1303T can be distinguished from other species of the genus Citeromyces by its inability to assimilate trehalose. The strain CLIB 1303T ( = CBS 12700T) was assigned to a novel species, Citeromyces nyonsensis sp. nov.

scholarly journals Teunia siamensis f.a., sp. nov., a novel tremellaceous yeast species isolated from soil in a secondary peat swamp forest area

2020 ◽  
Vol 70 (6) ◽  
pp. 3673-3678 ◽  
Author(s):  
Pannida Khunnamwong ◽  
Noppon Lertwattanasakul ◽  
Savitree Limtong
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Its Region ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Peat Swamp ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

The strain DMKU-XD44, representing an anamorphic novel yeast species, was isolated from soil collected in a peat swamp forest (PSF) area in Rayong Botanical Garden in eastern Thailand. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, Teunia cuniculi CBS 10309T was the most closely related species. The novel species differed from the T. cuniculi type by 2.5 % (14 nucleotide substitutions) in the D1/D2 region of the LSU rRNA gene and by 8.0 % (40 nucleotide substitutions) in the ITS region. The results of a phylogenetic analysis, based on the combined sequences of the ITS region and the D1/D2 region, indicated that DMKU-XD44 represents a member of the Teunia clade in the Cryptococcaceae (Tremellales, Tremellomycetes, Agaricomycotina and Basidiomycota) and is phylogenetically distinct from other species of the genus Teunia in the clade. Therefore, DMKU-XD44 represents a novel species of the genus Teunia. The name Teunia siamensis f.a., sp. nov. is proposed. The holotype is DMKU-XD44, while the MycoBank number is MB 832816.

scholarly journals Barnettozyma siamensis f.a., sp. nov., a lipid-accumulating ascomycete yeast species

2014 ◽  
Vol 64 (Pt_9) ◽  
pp. 3053-3057 ◽  
Author(s):  
Pirapan Polburee ◽  
Wichien Yongmanitchai ◽  
Takao Ohashi ◽  
Kazuhito Fujiyama ◽  
Savitree Limtong
Keyword(s):  
Type Strain ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Isolation Technique ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

Two strains, DMKU-UbN24(1)T and DMKU-CPN24(1), of a novel yeast species were obtained from soil and palm oil fruit, respectively, collected in Thailand by an enrichment isolation technique using a nitrogen-limited medium containing glycerol as the sole source of carbon. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, the two strains were found to represent a novel species of the genus Barnettozyma although the formation of ascospores was not observed. The novel species was related most closely to the type strain of Candida montana but differed by 5.4 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and by 10.3–10.5 % nucleotide substitutions in the ITS region. The name Barnettozyma siamensis f.a., sp. nov. is proposed. The type strain is DMKU-UbN24(1)T ( = BCC 61189T = NBRC 109701T = CBS 13392T).

scholarly journals Hannaella phyllophila sp. nov., a basidiomycetous yeast species associated with plants in Thailand and Taiwan

2015 ◽  
Vol 65 (Pt_7) ◽  
pp. 2135-2140 ◽  
Author(s):  
Janjira Surussawadee ◽  
Sasitorn Jindamorakot ◽  
Takashi Nakase ◽  
Ching-Fu Lee ◽  
Savitree Limtong
Keyword(s):  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Nucleotide Substitutions ◽  
Lsu Rrna Gene ◽  
Anamorphic Yeast ◽  
Lsu Rrna

Five strains representing one novel anamorphic yeast species were isolated from plant leaves collected in Thailand (strains DMKU-SP186T, ST-111 and ST-201) and Taiwan (strains FN20L02 and SM13L16). On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, they were assigned to a single novel species of the genus Hannaella. The sequences of the D1/D2 regions of the LSU rRNA genes of four of the strains (DMKU-SP186T, ST-111, FN20L02 and SM13L16) were identical, while differing from strain ST-201 by 2 substitutions and 2 gaps. The nucleotide sequence of the ITS regions of the five strains differed from each other by between 0 and 3 nucleotide substitutions. The novel species was most closely related to Hannaella luteola, but showed 1.0–1.3 % nucleotide substitutions (between 6 substitutions out of 568–606 nt and 8 substitutions, and 2 gaps out of 597 nt) in the D1/D2 region of the LSU rRNA gene and 1.4–2.0 % nucleotide substitutions (6–9 substitutions out of 435 nt) in the ITS region. Ballistospores were produced by three of the strains on cornmeal agar at 15 and 20 °C after 4 weeks, while H. luteola did not produce ballistospores. The name Hannaella phyllophila sp. nov. is proposed. The type strain is DMKU-SP186T ( = BCC 69500T = NBRC 110428T = CBS 13921T).

scholarly journals W ickerhamiella siamensis f.a., sp. nov., an endophytic and epiphytic yeast species isolated from sugar cane leaf

2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3849-3855 ◽  
Author(s):  
Pannida Khunnamwong ◽  
Janjira Surussawadee ◽  
Sasitorn Jindamorakot ◽  
Savitree Limtong
Keyword(s):  
Sugar Cane ◽  
Yeast Species ◽  
Novel Species ◽  
Its Region ◽  
Rrna Gene ◽  
The Novel ◽  
Nucleotide Substitutions ◽  
Lsu Rrna Gene ◽  
Lsu Rrna ◽  
Epiphytic Yeast

Six strains representing a novel yeast species were isolated from tissue (DMKU-SE106T, DMKU-SE110, DMKU-SE112 and DMKU-SE132) and the external surface (DMKU-SP335 and DMKU-SP406) of sugar cane leaves collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 region of the LSU rRNA gene and the internal transcribed spacer (ITS) region, the six strains were found to represent a single novel species of the genus Wickerhamiella although the formation of ascospores was not observed. The sequences of the D1/D2 region of the LSU rRNA gene and ITS region of the six strains differed from each other by 0–2 and 2–3 nt substitutions, respectively. The novel species was related most closely to Candida infanticola but with 4.5–4.6 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and 6.6–7.1 % nucleotide substitutions in the ITS region. The name Wickerhamiella siamensis f.a., sp. nov. is proposed. The type strain is DMKU-SE106T ( = BCC 61185T = NBRC 109697T = CBS 13331T).

Nakazawaea siamensis f.a., sp. nov., a yeast species isolated from phylloplane

2014 ◽  
Vol 64 (Pt_1) ◽  
pp. 266-270 ◽  
Author(s):  
Rungluk Kaewwichian ◽  
Savitree Limtong
Keyword(s):  
Type Strain ◽  
External Surface ◽  
Yeast Species ◽  
Novel Species ◽  
Its Region ◽  
Rrna Gene ◽  
The Novel ◽  
Nucleotide Substitutions ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

Strain DMKU-RK467T, representing a novel yeast species, was isolated from the external surface of sugar cane leaves collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, and sequence analysis of the D1/D2 region of the LSU rRNA gene and the internal transcribed spacer (ITS) region, strain DMKU-RK467T was assigned to a novel species of the genus Nakazawaea. The novel species was related most closely to the type strain of Candida wickerhamii but they differed by 1.9 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and by 5.2 % nucleotide substitutions in the ITS region. The name Nakazawaea siamensis f.a., sp. nov. is proposed (type strain DMKU-RK467T = BCC 50734T = NBRC 108903T = CBS 12569T).

Wickerhamomyces siamensis sp. nov., a novel yeast species isolated from the phylloplane in Thailand

2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1568-1573 ◽  
Author(s):  
Rungluk Kaewwichian ◽  
Hiroko Kawasaki ◽  
Savitree Limtong
Keyword(s):  
External Surface ◽  
Yeast Species ◽  
Novel Species ◽  
Large Subunit ◽  
Its Region ◽  
Rrna Gene ◽  
The Novel ◽  
Nucleotide Substitutions ◽  
Lsu Rrna Gene ◽  
Lsu Rrna

Strain DMKU-RK359T, representing a novel yeast species, was isolated from the external surface of a sugar-cane leaf collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 region of the large-subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, strain DMKU-RK359T was assigned to a novel Wickerhamomyces species. The novel species was closest to Wickerhamomyces ciferrii, but differed from it by 0.7 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and 6 % nucleotide substitutions in the ITS region. The name Wickerhamomyces siamensis sp. nov. is proposed (type strain DMKU-RK359T  = BCC 50732T  = NBRC 108900T  = CBS 12570T).

scholarly journals Dioszegia antarctica sp. nov. and Dioszegia cryoxerica sp. nov., psychrophilic basidiomycetous yeasts from polar desert soils in Antarctica

2010 ◽  
Vol 60 (6) ◽  
pp. 1466-1472 ◽  
Author(s):  
Laurie B. Connell ◽  
Regina Redman ◽  
Russel Rodriguez ◽  
Anne Barrett ◽  
Melissa Iszard ◽  
...  
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Fungal Population ◽  
Desert Soils ◽  
Polar Desert ◽  
Yeast Strains ◽  
Victoria Land ◽  
Lsu Rrna Gene

During a survey of the culturable soil fungal population in samples collected in Taylor Valley, South Victoria Land, Antarctica, 13 basidiomycetous yeast strains with orange-coloured colonies were isolated. Phylogenetic analyses of internal transcribed spacer (ITS) and partial LSU rRNA gene sequences showed that the strains belong to the Dioszegia clade of the Tremellales (Tremellomycetes, Agaricomycotina), but did not correspond to any of the hitherto recognized species. Two novel species, Dioszegia antarctica sp. nov. (type strain ANT-03-116T =CBS 10920T =PYCC 5970T) and Dioszegia cryoxerica sp. nov. (type strain ANT-03-071T =CBS 10919T =PYCC 5967T), are described to accommodate ten and three of these strains, respectively. Analysis of ITS sequences demonstrated intrastrain sequence heterogeneity in D. cryoxerica. The latter species is also notable for producing true hyphae with clamp connections and haustoria. However, no sexual structures were observed. The two novel species can be considered obligate psychrophiles, since they failed to grow above 20 °C and grew best between 10 and 15 °C.

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