A First Glimpse on Cold-Adapted PCB-Oxidizing Bacteria in Edmonson Point Lakes (Northern Victoria Land, Antarctica)

Antarctic freshwater ecosystems are especially vulnerable to human impacts. Polychlorobiphenyls (PCBs) are persistent organic pollutants that have a long lifetime in the environment. Despite their use having either been phased out or restricted, they are still found in nature, also in remote areas. Once in the environment, the fate of PCBs is strictly linked to bacteria which represent the first step in the transfer of toxic compounds to higher trophic levels. Data on PCB-oxidizing bacteria from polar areas are still scarce and fragmented. In this study, the occurrence of PCB-oxidizing cold-adapted bacteria was evaluated in water and sediment of four coastal lakes at Edmonson Point (Northern Victoria Land, Antarctica). After enrichment with biphenyl, 192 isolates were obtained with 57 of them that were able to grow in the presence of the PCB mixture Aroclor 1242, as the sole carbon source. The catabolic gene bphA, as a proxy for PCB degradation potential, was harbored by 37 isolates (out of 57), mainly affiliated to the genera Salinibacterium, Arthrobacter (among Actinobacteria) and Pusillimonas (among Betaproteobacteria). Obtained results enlarge our current knowledge on cold-adapted PCB-oxidizing bacteria and pose the basis for their potential application as a valuable eco-friendly tool for the recovery of PCB-contaminated cold sites.

Outlet Glacier and Landscape Evolution of Victoria Land, Antarctica

<p>Terrestrial cosmogenic exposure studies are an established and rapidly evolving tool for landscapes in both polar and non-polar regions. This thesis takes a multifaceted approach to utilizing and enhancing terrestrial cosmogenic methods. The three main components of this work address method development, reconstructing surface-elevation-changes in two large Antarctic outlet glaciers, and evaluating bedrock erosion rates in Victoria Land, Antarctica. Each facet of this work is intended to enhance its respective field, as well as benefit the other sections of this thesis. Quartz purification is a necessary and critical step to producing robust and reproducible results in terrestrial cosmogenic nuclide studies. Previous quartz purification work has centred on relatively coarse sample material (1 mm-500 μm) and is effective down to 125 μm. However, sample material finer than that poses significant purification challenges and this material is usually discarded. The new purification procedure outlined in this thesis shows that very fine sand size material (125-63 μm) can be reliably cleaned for use in terrestrial cosmogenic nuclide studies. The results below show that 35% mass loss in very fine-grained quartz is sufficient to remove major elements (Al, Ti, Na, K, Fe, Mg, Ca, Mn,) and trace elements (9Be, and 10B) along with meteoric 10Be. Insufficient leaching is most detrimental to Al concentration, however errors up to 27% in exposure age and up to 29% in erosion rate are possible if meteoric 10Be is not fully removed from quartz during the HF leaching stages. Outlet glaciers have been well observed since the beginning of the satellite era, approximately 60 years ago. However, we do not currently know how these important glaciers, which drain a significant portion of the Antarctic Ice Sheet, have behaved on centennial to millennial timescales. Dating glacial erratics deposited by a thinning outlet glacier provides a window into the long-term outlet glacier and ice sheet response to climatic forcing. New results in this thesis constrain the thinning history of Mawson and Tucker glaciers over the last several thousand years. Mawson Glacier undergoes rapid thinning from at least ~6.5 kya to ~4.9 kya then transitions to slower thinning until ~1 kya, with a minimum of 250 m of ice-surface-lowering. While Tucker Glacier ~450 km north undergoes gradual thinning from ~19 kya to ~5 kya with ~300 m of ice-surface-lowering. The results of this work show that either the Tucker Glacier was not significantly affected by the Ross Ice Shelf grounding line, or that Antarctic mountain glaciers respond differently to the outlet glaciers connected to the Easty Antarctic Ice Sheet. The style, rate, magnitude, and duration of thinning is unique to each outlet glacier, even with similar climate forcing. The results of this work shed light on the style and duration of outlet glacier thinning and retreat that is possible following a climate perturbation. Antarctica’s average bedrock erosion rate is consistently lower than 4.5 m/Myr, the lowest bedrock erosion rates for any region on Earth. Therefore, many cosmogenic dating studies assume zero erosion when calculating exposure ages. However, previous erosion rate work in Antarctica is biased to arid high-elevation inland sites (~60% of work) and the hyperarid ice-free McMurdo Dry Valleys (~40% of work). These studies do not capture the effects of coastal maritime climates, where many outlet glacier studies are conducted, on the rate of bedrock erosion. New results presented in this thesis show that the Northern Victoria Land coast has the highest known erosion rate in Antarctica. Two sample sites were selected, one coastal and one in the interior. The coastal bedrock erosion rates are 8.86±0.78 m/Myr and 7.15±0.6 m/Myr while the interior bedrock erosion rates are 1.07±0.08 m/Myr and 0.42±0.03 m/Myr. The coastal erosion rates are average for non-polar cold climates while the inland sites are below average for polar erosion rates. The results suggest a strong gradient in the rate of erosion is present from the Antarctic coastline inland. If exposure ages are not calculated with an appropriate erosion rate the apparent age may under-estimate the actual age by as much as 12%, which is thousands of years for Holocene thinning histories like those found in this thesis.</p>

Outlet Glacier and Landscape Evolution of Victoria Land, Antarctica

<p>Terrestrial cosmogenic exposure studies are an established and rapidly evolving tool for landscapes in both polar and non-polar regions. This thesis takes a multifaceted approach to utilizing and enhancing terrestrial cosmogenic methods. The three main components of this work address method development, reconstructing surface-elevation-changes in two large Antarctic outlet glaciers, and evaluating bedrock erosion rates in Victoria Land, Antarctica. Each facet of this work is intended to enhance its respective field, as well as benefit the other sections of this thesis. Quartz purification is a necessary and critical step to producing robust and reproducible results in terrestrial cosmogenic nuclide studies. Previous quartz purification work has centred on relatively coarse sample material (1 mm-500 μm) and is effective down to 125 μm. However, sample material finer than that poses significant purification challenges and this material is usually discarded. The new purification procedure outlined in this thesis shows that very fine sand size material (125-63 μm) can be reliably cleaned for use in terrestrial cosmogenic nuclide studies. The results below show that 35% mass loss in very fine-grained quartz is sufficient to remove major elements (Al, Ti, Na, K, Fe, Mg, Ca, Mn,) and trace elements (9Be, and 10B) along with meteoric 10Be. Insufficient leaching is most detrimental to Al concentration, however errors up to 27% in exposure age and up to 29% in erosion rate are possible if meteoric 10Be is not fully removed from quartz during the HF leaching stages. Outlet glaciers have been well observed since the beginning of the satellite era, approximately 60 years ago. However, we do not currently know how these important glaciers, which drain a significant portion of the Antarctic Ice Sheet, have behaved on centennial to millennial timescales. Dating glacial erratics deposited by a thinning outlet glacier provides a window into the long-term outlet glacier and ice sheet response to climatic forcing. New results in this thesis constrain the thinning history of Mawson and Tucker glaciers over the last several thousand years. Mawson Glacier undergoes rapid thinning from at least ~6.5 kya to ~4.9 kya then transitions to slower thinning until ~1 kya, with a minimum of 250 m of ice-surface-lowering. While Tucker Glacier ~450 km north undergoes gradual thinning from ~19 kya to ~5 kya with ~300 m of ice-surface-lowering. The results of this work show that either the Tucker Glacier was not significantly affected by the Ross Ice Shelf grounding line, or that Antarctic mountain glaciers respond differently to the outlet glaciers connected to the Easty Antarctic Ice Sheet. The style, rate, magnitude, and duration of thinning is unique to each outlet glacier, even with similar climate forcing. The results of this work shed light on the style and duration of outlet glacier thinning and retreat that is possible following a climate perturbation. Antarctica’s average bedrock erosion rate is consistently lower than 4.5 m/Myr, the lowest bedrock erosion rates for any region on Earth. Therefore, many cosmogenic dating studies assume zero erosion when calculating exposure ages. However, previous erosion rate work in Antarctica is biased to arid high-elevation inland sites (~60% of work) and the hyperarid ice-free McMurdo Dry Valleys (~40% of work). These studies do not capture the effects of coastal maritime climates, where many outlet glacier studies are conducted, on the rate of bedrock erosion. New results presented in this thesis show that the Northern Victoria Land coast has the highest known erosion rate in Antarctica. Two sample sites were selected, one coastal and one in the interior. The coastal bedrock erosion rates are 8.86±0.78 m/Myr and 7.15±0.6 m/Myr while the interior bedrock erosion rates are 1.07±0.08 m/Myr and 0.42±0.03 m/Myr. The coastal erosion rates are average for non-polar cold climates while the inland sites are below average for polar erosion rates. The results suggest a strong gradient in the rate of erosion is present from the Antarctic coastline inland. If exposure ages are not calculated with an appropriate erosion rate the apparent age may under-estimate the actual age by as much as 12%, which is thousands of years for Holocene thinning histories like those found in this thesis.</p>

Mid-Holocene thinning of David Glacier, Antarctica: chronology and controls

Quantitative satellite observations only provide an assessment of ice sheet mass loss over the last four decades. To assess long-term drivers of ice sheet change, geological records are needed. Here we present the first millennial-scale reconstruction of David Glacier, the largest East Antarctic outlet glacier in Victoria Land. To reconstruct changes in ice thickness, we use surface exposure ages of glacial erratics deposited on nunataks adjacent to fast-flowing sections of David Glacier. We then use numerical modelling experiments to determine the drivers of glacial thinning. Thinning profiles derived from 45 10Be and 3He surface exposure ages show David Glacier experienced rapid thinning of up to 2 m/yr during the mid-Holocene (∼ 6.5 ka). Thinning slowed at 6 ka, suggesting the initial formation of the Drygalski Ice Tongue at this time. Our work, along with ice thinning records from adjacent glaciers, shows simultaneous glacier thinning in this sector of the Transantarctic Mountains occurred 4–7 kyr after the peak period of ice thinning indicated in a suite of published ice sheet models. The timing and rapidity of the reconstructed thinning at David Glacier is similar to reconstructions in the Amundsen and Weddell embayments. To identify the drivers of glacier thinning along the David Glacier, we use a glacier flowline model designed for calving glaciers and compare modelled results against our geological data. We show that glacier thinning and marine-based grounding-line retreat are controlled by either enhanced sub-ice-shelf melting, reduced lateral buttressing or a combination of the two, leading to marine ice sheet instability. Such rapid glacier thinning events during the mid-Holocene are not fully captured in continental- or catchment-scale numerical modelling reconstructions. Together, our chronology and modelling identify and constrain the drivers of a ∼ 2000-year period of dynamic glacier thinning in the recent geological past.

Geology of the Sirius Group at Mount Feather and Table Mountain, South Victoria Land, Antarctica

<p>The Sirius Group comprises of wet based glacial and related deposits found at high elevations throughout the Transantarctic Mountains. The discovery of marine Pliocene diatoms from within glacial till by Harwood (1983) led Webb et al. (1984) to propose that they were sourced from diatom bearing sediment eroded by glaciers from middle Pliocene marine basins inland of the Transantarctic Mountains. Others consider that temperatures during middle Pliocene times were not high enough to melt back the Antarctic ice sheet and expose these inland basins. They support the long held view of a stable Antarctic ice sheet since middle Miocene times, and insist that the Sirius Group is much older, explaining the diatoms as wind blown. This study was undertaken in order to determine whether the diatoms were incorporated into Sirius Group tills during or after their deposition. Sites were sampled at Mount Feather and Table Mountain in South Victoria Land. The distribution of diatoms through the upper 37 cm of the till were documented. Samples were also taken in snow and from other non Sirius Group surfaces (regolith) for comparison purposes. The geomorphic setting of the Sirius Group tills at Mt. Feather and Table Mountain suggests that their deposition predated the deep valleys that now run through the Transantarctic Mountains. Diatom abundances from within the tills are low (averaging about 1 diatom diatom per gram) and highly variable from site to site. Initially 184 diatoms were recovered from 10 samples at Mt. Feather and less than 7 diatoms were found from 4 samples of till at Table Mountain. At Mount Feather diatoms are concentrated in the surface few centimetres of the till and numbers generally decrease with depth. The pore size within the tills is highly variable but is on average ten times the size of the average diatom (10-15 microns) from within the deposits, allowing at least some diatoms to work their way into the tills from the surface. Diatoms from the snow and regolith from other rock surfaces have a similar diatom assemblage to the Sirius tills, containing many of the same common forms. Some non Sirius Group regolith samples have much larger concentrations of diatoms suggesting they have a much better trapping ability than the Sirius Group tills. These data indicate that most diatoms from the Sirius Group tills have been introduced from the atmosphere and have worked their way into the till. Thus the Sirius diatoms record not Pliocene marine basins of the Antarctic interior and subsequent extensive over riding glaciation, but the atmospheric transport and collection of both modern and ancient diatom bearing dust from within and beyond the continent. The Sirius Group tills do however have a phytolith (siliceous clasts from the cells of plant tissue) flora of glaciogenic origin, indicated by the lack of a vertical trend in abundance and very low levels of phytoliths in nearby snow and regolith samples.</p>

Geology of the Sirius Group at Mount Feather and Table Mountain, South Victoria Land, Antarctica

<p>The Sirius Group comprises of wet based glacial and related deposits found at high elevations throughout the Transantarctic Mountains. The discovery of marine Pliocene diatoms from within glacial till by Harwood (1983) led Webb et al. (1984) to propose that they were sourced from diatom bearing sediment eroded by glaciers from middle Pliocene marine basins inland of the Transantarctic Mountains. Others consider that temperatures during middle Pliocene times were not high enough to melt back the Antarctic ice sheet and expose these inland basins. They support the long held view of a stable Antarctic ice sheet since middle Miocene times, and insist that the Sirius Group is much older, explaining the diatoms as wind blown. This study was undertaken in order to determine whether the diatoms were incorporated into Sirius Group tills during or after their deposition. Sites were sampled at Mount Feather and Table Mountain in South Victoria Land. The distribution of diatoms through the upper 37 cm of the till were documented. Samples were also taken in snow and from other non Sirius Group surfaces (regolith) for comparison purposes. The geomorphic setting of the Sirius Group tills at Mt. Feather and Table Mountain suggests that their deposition predated the deep valleys that now run through the Transantarctic Mountains. Diatom abundances from within the tills are low (averaging about 1 diatom diatom per gram) and highly variable from site to site. Initially 184 diatoms were recovered from 10 samples at Mt. Feather and less than 7 diatoms were found from 4 samples of till at Table Mountain. At Mount Feather diatoms are concentrated in the surface few centimetres of the till and numbers generally decrease with depth. The pore size within the tills is highly variable but is on average ten times the size of the average diatom (10-15 microns) from within the deposits, allowing at least some diatoms to work their way into the tills from the surface. Diatoms from the snow and regolith from other rock surfaces have a similar diatom assemblage to the Sirius tills, containing many of the same common forms. Some non Sirius Group regolith samples have much larger concentrations of diatoms suggesting they have a much better trapping ability than the Sirius Group tills. These data indicate that most diatoms from the Sirius Group tills have been introduced from the atmosphere and have worked their way into the till. Thus the Sirius diatoms record not Pliocene marine basins of the Antarctic interior and subsequent extensive over riding glaciation, but the atmospheric transport and collection of both modern and ancient diatom bearing dust from within and beyond the continent. The Sirius Group tills do however have a phytolith (siliceous clasts from the cells of plant tissue) flora of glaciogenic origin, indicated by the lack of a vertical trend in abundance and very low levels of phytoliths in nearby snow and regolith samples.</p>

An Analysis of the Temperature Profiles of Some Antarctic Lakes

<p>The temperature profiles of certain lakes in Taylor Valley, Victoria Land, Antarctica, are shown to be consistent with the hypothesis that these lakes were formerly cold brine pools; that their levels were raised by the addition of fresh water; and that they have since been heated principally by the absorption of sunlight. The temperature profile of a lake in Wright Valley, Victoria Land, is shown to be consistent with the hypothesis that this lake was formerly warm and stable, as are those Taylor Valley lakes which were analysed; that the addition of a further large quantity of fresh water caused instability and limited convection; and that the heat source is again absorbed sunlight. The study of this lake requires an understanding of convection in the presence of a gradient of solute concentration. A survey of existing knowledge of this type of convection shows that it is inadequate for the task. Experiments which provide the necessary information are described.</p>

An Analysis of the Temperature Profiles of Some Antarctic Lakes

<p>The temperature profiles of certain lakes in Taylor Valley, Victoria Land, Antarctica, are shown to be consistent with the hypothesis that these lakes were formerly cold brine pools; that their levels were raised by the addition of fresh water; and that they have since been heated principally by the absorption of sunlight. The temperature profile of a lake in Wright Valley, Victoria Land, is shown to be consistent with the hypothesis that this lake was formerly warm and stable, as are those Taylor Valley lakes which were analysed; that the addition of a further large quantity of fresh water caused instability and limited convection; and that the heat source is again absorbed sunlight. The study of this lake requires an understanding of convection in the presence of a gradient of solute concentration. A survey of existing knowledge of this type of convection shows that it is inadequate for the task. Experiments which provide the necessary information are described.</p>