scholarly journals Direct identification of bacteria from BacT/ALERT anaerobic positive blood cultures by MALDI-TOF MS: MALDI Sepsityper kit versus an in-house saponin method for bacterial extraction

2012 ◽  
Vol 61 (11) ◽  
pp. 1511-1516 ◽  
Author(s):  
Cécile Meex ◽  
Florence Neuville ◽  
Julie Descy ◽  
Pascale Huynen ◽  
Marie-Pierre Hayette ◽  
...  
Keyword(s):  
Blood Cultures ◽  
Direct Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Identification Of Bacteria ◽  
Tof Ms

scholarly journals Evaluation of a Rapid and Simplified Protocol for Direct Identification of Microorganisms From Positive Blood Cultures by Using Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)

2021 ◽  
Vol 11 ◽  
Author(s):  
Yufeng Dai ◽  
Xinyi Xu ◽  
Xue Yan ◽  
Daming Li ◽  
Wei Cao ◽  
...  
Keyword(s):  
Laser Desorption ◽  
Blood Cultures ◽  
Laser Desorption Ionization ◽  
Direct Identification ◽  
Ionization Time ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Matrix Assisted Laser

Early and rapid identification of microorganisms is critical for reducing the mortality rate caused by bloodstream infections (BSIs). The accuracy and feasibility of directly identifying pathogens in positive blood cultures by matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been intensely confirmed. In this study, we combined density centrifugation and extra chemical lysis-extraction to develop an optimized method in the blood culture process, which significantly improved the effectiveness of direct identification by MALDI-TOF MS. The accuracy was evaluated by 2,032 positive blood culture samples (115 species of microorganism). The overall MALDI-TOF MS based identification rate with scores ≥ 1.700 was 87.60%. 94.06% of gram-negative bacteria were identified consistently to the genus level, followed by anaerobes (93.33%), gram-positive bacteria (84.46%), and fungi (60.87%). This protocol could obtain results within 10–20 min at a cost of less than $0.1 per sample, which saved up to 24 h in identifying 87.60% of the microorganism from positive blood cultures. This rapid and simplified protocol facilitates the direct identification of microorganism in positive blood cultures, and exhibits the advantages of cost-effective, time-saving, and easy-to-use. It could provide the causative organism of the patient to clinicians in time for targeted treatment and reduce mortality.

scholarly journals Direct identification of microorganisms from positive blood cultures by MALDI-TOF MS using an in-house saponin method

2016 ◽  
Vol 52 ◽  
pp. 37-42 ◽  
Author(s):  
Shota Yonetani ◽  
Hiroaki Ohnishi ◽  
Kiyofumi Ohkusu ◽  
Tetsuya Matsumoto ◽  
Takashi Watanabe
Keyword(s):  
Blood Cultures ◽  
Direct Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

Facilitating effective treatment of septicaemia by direct identification of microorganisms in positive BacT/ALERT mds blood cultures using MALDI-TOF MS

Pathology ◽  
2018 ◽  
Vol 50 ◽  
pp. S115
Author(s):  
Christopher J. McIver ◽  
Noel Er ◽  
Chinmoy Mukerjee ◽  
Sophie Tokis ◽  
Peter C. Taylor
Keyword(s):  
Effective Treatment ◽  
Blood Cultures ◽  
Direct Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms

scholarly journals Lysis Centrifugation Method for the Direct Identification of Positive Blood Cultures Using MALDI-TOF MS

2017 ◽  
Vol 4 (suppl_1) ◽  
pp. S588-S588
Author(s):  
Hilal Al Sidairi ◽  
Khalifa BinKhamis ◽  
Joline Head ◽  
Ross Davidson
Keyword(s):  
Blood Cultures ◽  
Direct Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Centrifugation Method ◽  
Tof Ms

scholarly journals Cheap and rapid in-house method for direct identification of positive blood cultures by MALDI-TOF MS technology

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Maya Azrad ◽  
Yoram Keness ◽  
Orna Nitzan ◽  
Nina Pastukh ◽  
Linda Tkhawkho ◽  
...  
Keyword(s):  
Blood Cultures ◽  
Direct Identification ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Tof Ms
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