Isolation and full genomic characterization of Batai virus from mosquitoes, Italy 2009

In 2009, 2589 mosquitoes were collected in northwest Italy and screened for orthobunyavirus RNA by RT-PCR. One pool of Anopheles maculipennis complex mosquitoes was found to be positive and a virus was isolated from that pool. The isolate was identified as Batai virus (BATV) by sequencing. Previously, BATV was detected in Italy, but limited data and no prior isolates existed. Full-length sequences of the S, M and L segments were determined for the newly isolated Italian strain. For comparison, partial sequences were also determined for the BATV strain Calovo (former Czechoslovakia, 1960). Phylogenetic analyses revealed clustering of the newly derived Italian BATV along with a recent isolate from Germany and the historic strain Calovo. To the best of our knowledge, this represents the first isolation of BATV from Italy, which confirms a broader geographical distribution of BATV in Europe than was previously verified by isolation.

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Phylogenetic analysis of canine parvoviruses from Turkey

Medycyna Weterynaryjna ◽

10.21521/mw.6334 ◽

2020 ◽

Vol 76 (01) ◽

pp. 6334-2020

Author(s):

ZEYNEP AKKUTAY-YOLDAR ◽

TAYLAN KOÇ B.

Keyword(s):

High Mortality ◽

Phylogenetic Trees ◽

Clinical Symptoms ◽

Phylogenetic Analyses ◽

Canine Parvovirus ◽

Mortality And Morbidity ◽

Identity Match ◽

Genomic Characterization

Canine parvovirus (CPV) type 2 is the causative agent of acute hemorrhagic enteritis and high mortality in the affected dogs. Numerous studies have been done to understand the origin of the virus and to exhibit new variants and circulating strains. This report describes the detection and genomic characterization of CPV strains from indoor and outdoor dogs in Ankara, Turkey. Samples were sent to our laboratory due to clinical symptoms in puppies. We tested blood and swab samples to determine the presence of canine parvovirus (CPV) in three puppies and two adult dogs by reverse transcription-polymerase chain reaction (RT-PCR) using VP2 (capsid protein) region primers of canine parvoviruses. Following that, to provide molecular characterization data Maximum Likelihood (ML) method was used for phylogenetic analyses. Constructed phylogenetic trees from the aligned nucleotide sequences revealed that our CPV strains demonstrated high genetic similarities, with 100% identity match on nucleotide alignments with each other and classified in CPV-2b genotypes.They have placed on a monophyletic clade as a sister branch with CPV VAC S quantum with 98.9% nucleotide homology. Our findings suggest that CPV-2b is actual and frequently seen variant in Turkey and shows high similarities with other CPV variants and a bit less with FPVs in Turkey and around the world. CPV causes high mortality and morbidity in dogs and to develop effective vaccines for protection of dogs in Turkey where there are few numbers of studies that have been done, field strains should be isolated and characterised.

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Near Full-Length Genomic Characterization of Two Novel HIV-1 Unique Recombinant Forms (CRF01_AE/CRF07_BC) Among MSM in Shijiazhuang City, Hebei Province, China

AIDS Research and Human Retroviruses ◽

10.1089/aid.2021.0100 ◽

2021 ◽

Author(s):

Leilei Han ◽

Hanping Li ◽

Lijing Wang ◽

Lei Jia ◽

Jingwan Han ◽

...

Keyword(s):

Hebei Province ◽

Full Length ◽

Genomic Characterization ◽

Hiv 1

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African Basil (Ocimum gratissimum) Is a Reservoir of Divergent Begomoviruses in Uganda

Plant Disease ◽

10.1094/pdis-08-19-1675-re ◽

2020 ◽

Vol 104 (3) ◽

pp. 853-859

Author(s):

Happyness G. Mollel ◽

Joseph Ndunguru ◽

Peter Sseruwagi ◽

Titus Alicai ◽

John Colvin ◽

...

Keyword(s):

Mosaic Virus ◽

Phylogenetic Analyses ◽

Plant Viruses ◽

Wild Plants ◽

Bipartite Begomoviruses ◽

Ocimum Gratissimum ◽

Genomic Characterization ◽

Open Access Article

Begomoviruses are plant viruses that cause major losses to many economically important crops. Although they are poorly understood, begomoviruses infecting wild plants may have an important role as reservoirs in the epidemiology of viral diseases. This study reports the discovery and genomic characterization of three novel bipartite begomoviruses from wild and cultivated African basil (Ocimum gratissimum) plants collected in Uganda, East Africa. Based on the symptoms shown by the infected plants, the names proposed for these viruses are Ocimum yellow vein virus (OcYVV), Ocimum mosaic virus (OcMV), and Ocimum golden mosaic virus (OcGMV). Genome and phylogenetic analyses suggest that DNA-A of OcGMV is mostly related to begomoviruses infecting tomato in Africa, whereas those of OcYVV and OcMV are closely related to one another and highly divergent within the Old World begomoviruses. The DNA-A of all characterized begomovirus isolates are of a recombinant nature, revealing the role of recombination in the evolution of these begomoviruses. The viruses characterized here are the first identified in O. gratissimum and the first in Ocimum spp. in the African continent and could have important epidemiological consequences for cultivated basils and other important crops. [Formula: see text] Copyright © 2020 The Author(s). This is an open access article distributed under the CC BY 4.0 International license .

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Near Full-Length Genomic Characterization of a Novel HIV Type 1 CRF07_ BC/01_AE Recombinant in Men Who Have Sex with Men from Sichuan, China

AIDS Research and Human Retroviruses ◽

10.1089/aid.2013.0104 ◽

2013 ◽

Vol 29 (8) ◽

pp. 1173-1176 ◽

Cited By ~ 30

Author(s):

Huamian Wei ◽

Ling Su ◽

Yi Feng ◽

Xiang He ◽

Jenny His ◽

...

Keyword(s):

Full Length ◽

Genomic Characterization ◽

Hiv Type 1 ◽

Sex With Men

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Isolation and DNA characterization of a simian retrovirus 5 from a Japanese monkey (Macaca fuscata)

Journal of General Virology ◽

10.1099/vir.0.047621-0 ◽

2013 ◽

Vol 94 (5) ◽

pp. 955-959 ◽

Cited By ~ 5

Author(s):

Jun-Ichiro Takano ◽

Arlene Leon ◽

Miyoko Kato ◽

Yuko Abe ◽

Koji Fujimoto

Keyword(s):

Macaca Fuscata ◽

Direct Sequencing ◽

Phylogenetic Analyses ◽

Japanese Monkey ◽

Full Length ◽

Universal Primers ◽

Sequence Comparisons ◽

Dna Characterization

An SRV-like virus was isolated from a colony-born Japanese monkey. To identify this SRV-like virus, we designed universal primers at regions that were conserved among the reported SRV sequences in the 5′-LTR and the short ORF and we obtained plasmid clones containing the complete gag, prt, pol and env genes. The full-length sequences of the isolate were determined from the plasmids and by direct sequencing. Sequence comparisons and phylogenetic analyses indicated that this SRV-like virus had a sequence identical to the reported 626 bp of SRV-5. In this study, we isolated SRV5/JPN/2005/V1 from a Japanese monkey and characterized the full-length SRV-5 sequence.

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