Modern lineages of Mycobacterium tuberculosis were recently introduced in western India and demonstrate increased transmissibility

Background: Mycobacterium tuberculosis (Mtb) transmissibility may vary between lineages (or variants) and this may contribute to the slow decline of tuberculosis (TB) incidence. The objective of our study was to compare transmissibility across four major lineages (L1-4) of Mtb among participants from two cohort studies in Pune, India. Methods: We performed whole-genome sequencing (WGS) of Mtb sputum culture-positive isolates from participants in two prospective cohort studies of adults with pulmonary TB seeking care at public treatment centers in Pune, Maharashtra. We performed genotypic susceptibility prediction for both first- and second-line drugs using a previously validated random forest model. We used single nucleotide substitutions (SNS) and maximum likelihood estimation to build isolate phylogenies by lineage. We used Bayesian molecular dating to estimate ancestral node ages and compared tree characteristics using a two-sample Kolmogorov-Smirnov (KS) test. Results: Of the 642 isolates from distinct study participants that underwent WGS, 612 met sequence quality criteria. The median age of the 612 participants was 31 years (IQR 24.4-44.2), the majority were male (64.7%) and sputum smear-positive (83.3%), and 6.7% had co-infection with HIV. Most isolates belonged to L3 (44.6%). The majority (61.1%) of multidrug-resistant isolates (MDR, resistant to isoniazid and rifampin) belonged to L2 (P < 0.001 [Fisher's Exact]). There was no significant difference in host characteristics between participants infected with the four major lineages. In phylogenetic analysis, we measured shorter terminal branch lengths in the L2 tree compared to L1 and L3 trees indicating less time elapsing between transmission and sampling and higher transmissibility (median branch lengths: L2 - 3.3, L3 - 7.8, p <0.001). Branching times for L2 and L4 were more recent than L1 and L3 indicating recent introduction into the region (p < 0.01 [KS test]). Conclusion: Modern Mtb lineages (L2 and L4) were more recently introduced in western India, compared to older lineages (L1 and L3). L2 shows a higher frequency of drug-resistance and higher transmissibility. Our findings highlight the need for contact tracing around cases of TB due to L2, and heightened surveillance of TB antibiotic resistance in India.

Soil Fertility Properties, Leaf Nutrient Status and Yield Of Coconut and Intercrops as Influenced and Coconut Based Cropping System in Coastal Plain of Western India

An experiment was conducted during 2013 -2019 to study the impact of intergrated nutrient management through organic matter recycling (OMR) on the soil properties, leaf nutrient status and yield of coconut and its components crops at Regional Coconut Research Station (AICRP on Palms), Bhatye, Maharashtra, India. The experiment was laid out in 0.40 ha with four treatments namely T1, T2, T3 and T4. Results showed that, INM practices significantly improved the soil physico-chemical, biological properties and leaf nutrient status, whereas it was more prominent for the T1 treatment. The soil organic carbon content during post experimental period gradually increased in the organic recyclable treatment under cropping system (0.80 to 0.86%) compared to monocropping (0.60%). The recyclable biomass produced from the system varied between 9.0 - 18.0 t/ha/year and vermicompost produced was 4.8 to 10.2 t/ha/year by composting through earthworm. The nut yield (147.2 and 138.4 nuts/palm), copra output (25.75 and 23.06 kg/palm) and oil output (17.11 and 15.72 kg/palm) under treatment T1 and T2 were on par and differed significantly compared to monocrop. Bangladesh J. Bot. 50(4): 1067-1075, 2021 (December)

Late quaternary palaeoclimates of western India : A geoarchaeological approach

Western India comprises parts of the states of Rajasthan, Gujarat and Maharashtra. This region has varied climate and landscape. Northwestern Rajasthan is an area of internal drainage, with dunes, playas and rocky pediments. The Luni, Sabarmati and Mahi basins to the south of this zone show the influence of both aeolian and fluvial processes. Aeolian landforms are absent in the Nrmada, Tapi, Godavari and Bhima basins and fluvial aggradation has alternated with erosion in response to climatic change. Dominance of semi-arid to arid climate throughtout the Quaternary in the entire region has ment that Quaternary geological processes have been weak, preserving many features of the Tertiary in the present landscape. The variation in the geomorphic processes shaping the landscape and the complex links between geomorphic proceses and climate mean that our understanding of palaeoclimates in western India in far from complete. This paper gives an overview of the Late Quaternary palaeoclimate of western India, based primarily on recent work by the authors in Rajasthan and Maharashtra. Some issues in the palaeoclimatic interpretation of the geological record are discussed. Signatures of Late Pleistocene aridity, fluctuating climate during the Pleistocene Holocene transitional period, early Holocene humid climate and increased aridity from the middle Holocene onwards are reflected in different ways in the different landscape settings.

The effect of Indian Ocean warming on the Indian Monsoon: An atmospheric model study

The results from an atmospheric modeling study using the Florida State University Global Spectral Model indicate that, in years such as 1997 when the Indian Ocean SSTs are large, the Indian monsoon exhibits a typical behaviour. During that year, an extended shift of the tropical convergence zone towards the north played a role in the regional Hadley cell anomalies. The local warm boundary conditions in the northwestern Indian Ocean aided the high rainfall anomaly in Western India during the model simulations. The upper level structure, exhibited in terms of the global velocity potential is slightly shifted east for 1997, but with the correct sign. This structure shows regions of convergence over Indonesia where severe drought had occurred. The performance of the model rainfall over the equatorial Indian Ocean was uncanny for most seasons studied. Overall, the model performed best over the oceanic regions.

COVID-19 among pregnant women: management outcome and risk of transmission to newborns in western India

Background: The WHO declared a global pandemic of coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 in March 2020. With so much female population in reproductive period in India the effect of corona virus in pregnancy warrants attention as the impact of maternal infection during pregnancy in India remains poorly understood. Objectives of current study were to know the management outcome and risk of transmission among COVI-19 positive pregnant women admitted in a tertiary care institute in western India.Materials: The present cross sectional study was conducted among 217 admitted COVID-19 positive pregnant women in a tertiary care institute in western India from May 2020 to April 2021. Data were collected from the department of obstetrics and gynaecology after taking permission from the head of department of obstetrics and gynaecology. Results: Most of patients were asymptomatic and managed conservatively but some also needed special interventions. Out of 217 patients 124 patients delivered in the hospital, 5 maternal deaths, one stillbirth and one congenital anomaly was reported. Only 25% of patients had normal delivery rest had caesarean and one neonate was found COVID-19 positive. Around one forth deliveries were pre term.Conclusions: The study suggests that most women do not have severe illness and that transmission of infection to infants of infected mothers is uncommon but there are increased chances of pre-term delivery. Majority of the women and neonates were managed and discharged without any major complications. But in some cases COVID in pregnancy may complicate the situation; so it should be handled by a multidisciplinary team of experts in COVID-19.