Nuclear progesterone receptor regulates blood coagulation facilitating ovulation in zebrafish

Ovulation is a remodeling process including blood capillary rupture and coagulation. Until now, there is no regulation and functional studies of coagulation factors in ovulation. Here, we report dramatic increases of coagulation factors (f5, f3a) in zebrafish preovulatory follicles. This upregulation was induced by progestin (DHP: 17α, 20β-dihydroxy-4-pregnen-3-one), a native ligand for nuclear progestin receptor (Pgr) that is essential for ovulation in zebrafish; but was abolished in pgr-/-. In addition, promoter activities of f5 and f3a were significantly enhanced by progestin via zebrafish Pgr. Similarly, we found promoter activities of human F5 were significantly stimulated by progesterone (P4) via human PGRB. Moreover, a dramatic increase of erythrocyte numbers in capillaries on ovarian follicles was associated with ovulation. Importantly, heparin, an anticoagulant, inhibited ovulation. Furthermore, reduced fecundity and impaired ovulation were observed in f5+/- female zebrafish. Together, our results provide plausible evidence for an exceptional function of coagulation factors in ovulation.

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Natural Oestrogen in the Female Climacteric - Influence on Blood Coagulation and Fibrinolysis

Thrombosis and Haemostasis ◽

10.1055/s-0038-1648686 ◽

1978 ◽

Vol 40 (02) ◽

pp. 532-541 ◽

Cited By ~ 2

Author(s):

Anders Lagrelius ◽

Nils-Olov Lunell ◽

Margareta Blombäck

Keyword(s):

Blood Coagulation ◽

Antithrombin Iii ◽

Coagulation Factors ◽

Control Group ◽

Blood Coagulation Factors ◽

Excessive Bleeding ◽

Oestrone Sulphate ◽

Menopausal Women ◽

History Of ◽

Coagulation And Fibrinolysis

SummaryThe aim of the present study was to investigate the effect on blood coagulation and fibrinolysis of a natural oestrogen preparation, piperazine oestrone sulphate, prospectively in menopausal women. Scopolamine was given to the control group.The women were investigated before and during treatment with regard to factors VIII, VII, X, V, fibrinopeptide A, antithrombin III, plasminogen, rapid antiplasmin and α1-antitrypsin. There was no significant change towards hypercoagulability or decreased fibrinolysis in any group. In the oestrogen group, however, a tendency towards an increased level of plasminogen and a decreased level of antiplasmin was demonstrated. In the scopolamine group there was an unexpected fall in factors X and V and also in plasminogen and α1,-antitrypsin. A low level of some blood coagulation factors in some of the women before treatment is somewhat astonishing; none of them had any history of excessive bleeding.

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Changes in Blood Coagulation and Fibrinolysis in Rats Fed Atherogenic Diets

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654785 ◽

1963 ◽

Vol 10 (02) ◽

pp. 295-308 ◽

Cited By ~ 8

Author(s):

Clarence Merskey ◽

Herbert Wohl

Keyword(s):

Blood Coagulation ◽

Blood Platelets ◽

Coagulation Factors ◽

Atherogenic Diet ◽

Normal Serum ◽

Significant Prolongation ◽

Lysis Time ◽

Factor Ii ◽

Euglobulin Lysis Time ◽

Coagulation And Fibrinolysis

Summary1. Groups of rats were fed thrombogenic diets and the effects on blood coagulation and fibrinolysis assessed.2. Animals fed a diet containing cholesterol, thiouracil and cholic acid developed high levels of coagulation factors I, II, V, VII—X, VIII, IX and X.3. Animals fed a similar diet with additional 40% beef fat developed even greater elevation of V, VII—X, VIII and X, similar elevation of factor II, and lesser (but still significant) elevation of factors I and IX. In addition marked elevation of blood platelets occurred.4. Euglobulin lysis time of the group not fed the additional fat was longer than in controls. Significant prolongation of euglobulin lysis time was not found in the group fed additional fat.5. If the increased levels of plasma fibrinogen were taken into account, it was found that a larger amount of fibrin was lysed per unit time in the euglobulin lysis test with plasma from rats fed either atherogenic diet compared with controls.6. Defective thromboplastin generation was present in both groups of rats fed an atherogenic diet. The defect was present in the serum and was not due to lack of a factor required for thromboplastin generation. An inhibitor was present in the serum which was capable of preventing the action of normal serum.7. No good correlation was found between the occurrence of changes in blood coagulation or fibrinolysis and the presence or absence of thrombosis and infarction.8. The exact cause of these anomalies remains unexplained, as does the cause of the thrombosis in these animals. Starvation per se does not account for these abnormal findings. They could not adequately be explained on the basis of “hypercoagulability” of the blood.

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STEROIDSTRUKTUR UND LEBERTOXIZITÄT

Acta Endocrinologica ◽

10.1530/acta.0.0540073 ◽

1967 ◽

Vol 54 (1) ◽

pp. 73-84 ◽

Cited By ~ 7

Author(s):

H. L. Krüskemper ◽

G. Noell

Keyword(s):

Alkaline Phosphatase ◽

Liver Function ◽

Blood Coagulation ◽

Electrophoretic Pattern ◽

Coagulation Factors ◽

Blood Coagulation Factors ◽

Methyl Group ◽

Liver Functions ◽

Male Subjects ◽

Androstane Derivatives

ABSTRACT In male subjects investigations have been carried out regarding the effect of C1- and C17-methylated androstane derivatives (20 mg per day, orally, two weeks) on liver functions (parameters: activities of GPT, GOT, alkaline phosphatase and cholinesterase in serum; electrophoretic pattern; blood coagulation factors V, VII, X and prothrombin; BSP-retention). In addition to the well known hepatotropic action of 17α-alkylated C-19-steroids a quasi-axial 1α-methyl configuration (in 1α-methylandrost-2-en-17β-ol) definitely increased BSP-retention and several coagulation factors. These steroid effects decreased gradually when a methyl group was introduced in C1 equatorially (1-methylandrost-1-en-17β-ol-3-one) or quasi-equatorially (1β-methylandrost-2-en-17β-ol), the latter compound completely lacking from any influence on parameters of liver function under investigation.

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Localization of the structural difference between bovine blood coagulation factors X1 and X2 to tyrosine 18 in the activation peptide.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)35614-4 ◽

1986 ◽

Vol 261 (9) ◽

pp. 4008-4014 ◽

Cited By ~ 2

Author(s):

T Morita ◽

C M Jackson

Keyword(s):

Blood Coagulation ◽

Structural Difference ◽

Coagulation Factors ◽

Bovine Blood ◽

Blood Coagulation Factors ◽

Activation Peptide

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EXPRESSION IN ONTOGENESIS OF HUMAN BLOOD COAGULATION FACTORS

10.1055/s-0038-1644610 ◽

1987 ◽

Author(s):

H J Hassan ◽

A Leonardi ◽

C Chelucci ◽

R Guerriero ◽

P M Mannucci ◽

...

Keyword(s):

Blood Coagulation ◽

Protein C ◽

Antithrombin Iii ◽

Liver Homogenate ◽

Coagulation Factor ◽

Coagulation Factors ◽

Factor Ix ◽

Adult Liver ◽

Blood Coagulation Factors ◽

Guanidine Isothiocyanate

We have analyzed the expression of several blood coagulation factors (IX, VIII, X, fibrinogen chains) and inhibitors (antithrombin III, protein C) in human embryonic and fetal livers, obtained from legal abortions at 6-11 week post-conception. The age was established by morphologic staging and particularly crown-rump lenght measurement.Total cellular RNA was isolated from partially purified hepatocytes or total liver homogenate using the guanidine isothiocyanate method. Poly(A)+ RNA was selected by oligodT cellulose chromatography. The size and the number of the embryonic and fetal transcripts are equivalent to those observed in adult liver, as evaluated by Northern blot analysis of total or poly(A)+ RNA hybridized to human cDNA probes.The level of coagulation factor transcripts in embryonic and fetal liver was evaluated by dot hybridization of total RNA (0.5-10 ug), as compared to RNA extracted from normal adult liver biopsies. The expression of blood coagulation factors in embryos is generally reduced for all factors, but at a different degree. In 5-11 wk liver, the level of factor IX is 5-10% of that observed in adults, while fibrinogen, protein C, antithrombin III RNA level rises from 25 to 50% and factor X is expressed at a level comparable to that observed in adult liver.We conclude that during these stages of development blood coagulation factors are expressed according to three different time, curves, possibly due to the effect of different types of regulatory mechanisms.

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