scholarly journals Sensory neuron-derived Nav1.7 contributes to dorsal horn neuron excitability

2019 ◽  
Author(s):  
Sascha R.A. Alles ◽  
Filipe Nascimento ◽  
Rafael Luján ◽  
Queensta Millet ◽  
Ali Bangash ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Functional Role ◽  
Dorsal Horn Neuron ◽  
Spiking Neurons ◽  
Pain Sensation ◽  
Tonic Firing ◽  
Dorsal Horn Neurons ◽  
Immuno Electron Microscopy ◽  
Horn Neuron

SummaryExpression of the voltage-gated sodium channel Nav1.7 in sensory neurons is required for pain sensation. We examined the role of Nav1.7 in the dorsal horn of the spinal cord using an epitope-tagged knock-in mouse. Immuno-electron microscopy showed the presence of Nav1.7 in dendrites of lamina II neurons, despite the absence of mRNA. Peripheral nervous system-specific Nav1.7 KO mice showed central deficits with lamina II dorsal horn tonic firing neurons more than halved and single spiking neurons more than doubled. Nav1.7 blocker PF05089771 diminished excitability in dorsal horn neurons, but had no effect on Nav1.7 KO mice. These data demonstrate an unsuspected functional role of peripherally generated Nav1.7 in dorsal horn neurons and an expression pattern that would not be predicted by transcriptomic analysis.

scholarly journals Sensory neuron–derived NaV1.7 contributes to dorsal horn neuron excitability

2020 ◽  
Vol 6 (8) ◽  
pp. eaax4568 ◽  
Author(s):  
Sascha R. A. Alles ◽  
Filipe Nascimento ◽  
Rafael Luján ◽  
Ana P. Luiz ◽  
Queensta Millet ◽  
...  
Keyword(s):  
Dorsal Horn ◽  
Dorsal Horn Neuron ◽  
Mutant Mice ◽  
Afferent Neuron ◽  
Null Mutant ◽  
Dorsal Horn Neurons ◽  
Afferent Nerves ◽  
Horn Neuron ◽  
Null Mutant Mice

Expression of the voltage-gated sodium channel NaV1.7 in sensory neurons is required for pain sensation. We examined the role of NaV1.7 in the dorsal horn of the spinal cord using an epitope-tagged NaV1.7 knock-in mouse. Immuno–electron microscopy showed the presence of NaV1.7 in dendrites of superficial dorsal horn neurons, despite the absence of mRNA. Rhizotomy of L5 afferent nerves lowered the levels of NaV1.7 in the dorsal horn. Peripheral nervous system–specific NaV1.7 null mutant mice showed central deficits, with lamina II dorsal horn tonic firing neurons more than halved and single spiking neurons more than doubled. NaV1.7 blocker PF05089771 diminished excitability in dorsal horn neurons but had no effect on NaV1.7 null mutant mice. These data demonstrate an unsuspected functional role of primary afferent neuron-generated NaV1.7 in dorsal horn neurons and an expression pattern that would not be predicted by transcriptomic analysis.

Role of D-serine in superficial dorsal horn neuron

PAIN RESEARCH ◽  
2011 ◽  
Vol 26 (1) ◽  
pp. 19-28
Author(s):  
Yasuhiko Kawasaki ◽  
Terumasa Nakatsuka ◽  
Mika Sasaki ◽  
Fumimasa Amaya ◽  
Tatsuro Kohno
Keyword(s):  
Dorsal Horn ◽  
Dorsal Horn Neuron ◽  
Superficial Dorsal Horn ◽  
Horn Neuron

scholarly journals Electroacupuncture reduces the evoked responses of the spinal dorsal horn neurons in ankle-sprained rats

2011 ◽  
Vol 105 (5) ◽  
pp. 2050-2057 ◽  
Author(s):  
Jae Hyo Kim ◽  
Hee Young Kim ◽  
Kyungsoon Chung ◽  
Jin Mo Chung
Keyword(s):  
Dorsal Horn ◽  
Ankle Sprain ◽  
Weight Bearing ◽  
Inhibitory Effect ◽  
Dorsal Horn Neuron ◽  
Lumbar Spinal Cord ◽  
Unit Recording ◽  
Dorsal Horn Neurons ◽  
Horn Neuron

Acupuncture is shown to be effective in producing analgesia in ankle sprain pain in humans and animals. To examine the underlying mechanisms of the acupuncture-induced analgesia, the effects of electroacupuncture (EA) on weight-bearing forces (WBR) of the affected foot and dorsal horn neuron activities were examined in a rat model of ankle sprain. Ankle sprain was induced manually by overextending ligaments of the left ankle in the rat. Dorsal horn neuron responses to ankle movements or compression were recorded from the lumbar spinal cord using an in vivo extracellular single unit recording setup 1 day after ankle sprain. EA was applied to the SI-6 acupoint on the right forelimb (contralateral to the sprained ankle) by trains of electrical pulses (10 Hz, 1-ms pulse width, 2-mA intensity) for 30 min. After EA, WBR of the sprained foot significantly recovered and dorsal horn neuron activities were significantly suppressed in ankle-sprained rats. However, EA produced no effect in normal rats. The inhibitory effect of EA on hyperactivities of dorsal horn neurons of ankle-sprained rats was blocked by the α-adrenoceptor antagonist phentolamine (5 mg/kg ip) but not by the opioid receptor antagonist naltrexone (10 mg/kg ip). These data suggest that EA-induced analgesia in ankle sprain pain is mediated mainly by suppressing dorsal horn neuron activities through α-adrenergic descending inhibitory systems at the spinal level.

Electrical stimulation of the primary somatosensory cortex inhibits spinal dorsal horn neuron activity

2005 ◽  
Vol 1057 (1-2) ◽  
pp. 134-140 ◽  
Author(s):  
Arun K. Senapati ◽  
Paula J. Huntington ◽  
Stacey C. LaGraize ◽  
Hilary D. Wilson ◽  
Perry N. Fuchs ◽  
...  
Keyword(s):  
Electrical Stimulation ◽  
Somatosensory Cortex ◽  
Dorsal Horn ◽  
Spinal Dorsal Horn ◽  
Dorsal Horn Neuron ◽  
Neuron Activity ◽  
Spinal Dorsal ◽  
Spinal Dorsal Horn Neuron ◽  
Horn Neuron ◽  
Stimulation Of

Multiple Firing Patterns in Deep Dorsal Horn Neurons of the Spinal Cord: Computational Analysis of Mechanisms and Functional Implications

2010 ◽  
Vol 104 (4) ◽  
pp. 1978-1996 ◽  
Author(s):  
Yann Le Franc ◽  
Gwendal Le Masson
Keyword(s):  
Spinal Cord ◽  
Dorsal Horn ◽  
Information Transfer ◽  
Network Effects ◽  
Neuronal Response ◽  
Sensory Information ◽  
Firing Patterns ◽  
Dorsal Horn Neurons ◽  
The Impact

Deep dorsal horn relay neurons (dDHNs) of the spinal cord are known to exhibit multiple firing patterns under the control of local metabotropic neuromodulation: tonic firing, plateau potential, and spontaneous oscillations. This work investigates the role of interactions between voltage-gated channels and the occurrence of different firing patterns and then correlates these two phenomena with their functional role in sensory information processing. We designed a conductance-based model using the NEURON software package, which successfully reproduced the classical features of plateau in dDHNs, including a wind-up of the neuronal response after repetitive stimulation. This modeling approach allowed us to systematically test the impact of conductance interactions on the firing patterns. We found that the expression of multiple firing patterns can be reproduced by changes in the balance between two currents (L-type calcium and potassium inward rectifier conductances). By investigating a possible generalization of the firing state switch, we found that the switch can also occur by varying the balance of any hyperpolarizing and depolarizing conductances. This result extends the control of the firing switch to neuromodulators or to network effects such as synaptic inhibition. We observed that the switch between the different firing patterns occurs as a continuous function in the model, revealing a particular intermediate state called the accelerating mode. To characterize the functional effect of a firing switch on information transfer, we used correlation analysis between a model of peripheral nociceptive afference and the dDHN model. The simulation results indicate that the accelerating mode was the optimal firing state for information transfer.

Role of cervical neurons in propriospinal inhibition of thoracic dorsal horn neurons

1992 ◽  
Vol 599 (2) ◽  
pp. 302-308 ◽  
Author(s):  
Lawrence R. Poree ◽  
Lawrence P. Schramm
Keyword(s):  
Dorsal Horn ◽  
Dorsal Horn Neurons

Dissection, Plating, and Maintenance of Dorsal Horn Neuron Cultures

2009 ◽  
Vol 2009 (8) ◽  
pp. pdb.prot5274-pdb.prot5274 ◽  
Author(s):  
C. Albuquerque ◽  
D. J. Joseph ◽  
P. Choudhury ◽  
A. B. MacDermott
Keyword(s):  
Dorsal Horn ◽  
Dorsal Horn Neuron ◽  
Horn Neuron
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