scholarly journals Gene expression profiling of single cells from archival tissue with laser-capture microdissection and Smart-3SEQ

2019 ◽  
Vol 29 (11) ◽  
pp. 1816-1825 ◽  
Author(s):  
Joseph W. Foley ◽  
Chunfang Zhu ◽  
Philippe Jolivet ◽  
Shirley X. Zhu ◽  
Peipei Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Laser Capture Microdissection ◽  
Expression Profiling ◽  
Single Cells ◽  
Archival Tissue ◽  
Laser Capture

scholarly journals Gene-expression profiling of single cells from archival tissue with laser-capture microdissection and Smart-3SEQ

2017 ◽  
Author(s):  
Joseph W. Foley ◽  
Chunfang Zhu ◽  
Philippe Jolivet ◽  
Shirley X. Zhu ◽  
Peipei Lu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Laser Capture Microdissection ◽  
Expression Profiling ◽  
Single Cells ◽  
Ffpe Tissue ◽  
Small Samples ◽  
Clinical Samples ◽  
Rna Seq ◽  
Laser Capture

AbstractRNA sequencing (RNA-seq) is a sensitive and accurate method for quantifying gene expression. Small samples or those whose RNA is degraded, such as formalin-fixed, paraffin-embedded (FFPE) tissue, remain challenging to study with nonspecialized RNA-seq protocols. Here we present a new method, Smart-3SEQ, that accurately quantifies transcript abundance even with small amounts of total RNA and effectively characterizes small samples extracted by laser-capture microdissection (LCM) from FFPE tissue. We also obtain distinct biological profiles from FFPE single cells, which have been impossible to study with previous RNA-seq protocols, and we use these data to identify possible new macrophage phenotypes associated with the tumor microenvironment. We propose Smart-3SEQ as a highly cost-effective method to enable large gene-expression profiling experiments unconstrained by sample size and tissue availability. In particular, Smart-3SEQ’s compatibility with FFPE tissue unlocks an enormous number of archived clinical samples, and combined with LCM it allows unprecedented studies of small cell populations and single cells isolated by their in situ context.

scholarly journals Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer

2007 ◽  
Vol 8 (1) ◽  
pp. 25 ◽  
Author(s):  
Dagmar M Kube ◽  
Cemile D Savci-Heijink ◽  
Anne-Françoise Lamblin ◽  
Farhad Kosari ◽  
George Vasmatzis ◽  
...  
Keyword(s):  
Gene Expression ◽  
Prostate Cancer ◽  
Gene Expression Profiling ◽  
Laser Capture Microdissection ◽  
Expression Profiling ◽  
Rna Amplification ◽  
Laser Capture

scholarly journals Gene expression profiling of gastric cancer by microarray combined with laser capture microdissection

2005 ◽  
Vol 11 (47) ◽  
pp. 7405 ◽  
Author(s):  
Ming-Shiang Wu ◽  
Yi-Shing Lin ◽  
Yu-Ting Chang ◽  
Chia-Tung Shun ◽  
Ming-Tsan Lin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Gene Expression Profiling ◽  
Laser Capture Microdissection ◽  
Expression Profiling ◽  
Laser Capture

scholarly journals Gene expression profiling of gastric cancer by microarray combined with laser capture microdissection

2005 ◽  
Vol 11 (47) ◽  
pp. 7405 ◽  
Author(s):  
Ming-Shiang Wu ◽  
Yi-Shing Lin ◽  
Yu-Ting Chang ◽  
Chia-Tung Shun ◽  
Ming-Tsan Lin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Gene Expression Profiling ◽  
Laser Capture Microdissection ◽  
Expression Profiling ◽  
Laser Capture

Gene expression profiling of individual hypothalamic nuclei from single animals using laser capture microdissection and microarrays

2009 ◽  
Vol 177 (1) ◽  
pp. 87-93 ◽  
Author(s):  
Sarah Juel Paulsen ◽  
Leif Kongskov Larsen ◽  
Jacob Jelsing ◽  
Uwe Janßen ◽  
Bernhard Gerstmayer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Laser Capture Microdissection ◽  
Expression Profiling ◽  
Hypothalamic Nuclei ◽  
Laser Capture

scholarly journals Gene expression profiling of the hyperplastic growth zones of the late trout embryo myotome using laser capture microdissection and microarray analysis

BMC Genomics ◽  
2013 ◽  
Vol 14 (1) ◽  
pp. 173 ◽  
Author(s):  
Pierre-Yves Rescan ◽  
Jerome Montfort ◽  
Alain Fautrel ◽  
Cécile Rallière ◽  
Veronique Lebret
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Microarray Analysis ◽  
Laser Capture Microdissection ◽  
Expression Profiling ◽  
Growth Zones ◽  
Laser Capture
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