Purification of Yeast Artificial Chromosome DNA for Microinjection Using a Two-Gel Electrophoresis Procedure

2018 ◽  
Vol 2018 (8) ◽  
pp. pdb.prot093930 ◽  
Author(s):  
Lluis Montoliu
Keyword(s):  
Gel Electrophoresis ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome

Long-range mapping of the 11q23 region involved in chromosome aberrations in human tumors by pulsed-field gel electrophoresis with a yeast artificial chromosome

1993 ◽  
Vol 8 (3) ◽  
pp. 167-171 ◽  
Author(s):  
Yukihiro Akao ◽  
Yoshihide Tsujimoto ◽  
Masao Seto ◽  
Takashi Imai ◽  
Dominique Bergeron ◽  
...  
Keyword(s):  
Long Range ◽  
Chromosome Aberrations ◽  
Gel Electrophoresis ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Pulsed Field Gel Electrophoresis ◽  
Human Tumors ◽  
Pulsed Field

Construction of yeast artificial chromosome libraries by pulsed-field gel electrophoresis

1994 ◽  
Vol 1 (3) ◽  
pp. 241-249
Author(s):  
Anthony P. Monaco ◽  
Zoia Larin ◽  
Hans Lehrach
Keyword(s):  
Gel Electrophoresis ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Construction of yeast artificial chromosome libraries with large inserts using fractionation by pulsed-field gel electrophoresis

1989 ◽  
Vol 17 (9) ◽  
pp. 3425-3433 ◽  
Author(s):  
Rakesh Anand ◽  
Alfredo Villasante ◽  
Chris Tyler-Smith
Keyword(s):  
Gel Electrophoresis ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field

scholarly journals Structure of the Chromosome VII Centromere Region in Neurospora crassa: Degenerate Transposons and Simple Repeats

1998 ◽  
Vol 18 (9) ◽  
pp. 5465-5477 ◽  
Author(s):  
Edward B. Cambareri ◽  
Rafael Aisner ◽  
John Carbon
Keyword(s):  
Neurospora Crassa ◽  
Yeast Artificial Chromosome ◽  
Fragment Length Polymorphism ◽  
Artificial Chromosome ◽  
Polymorphism Analysis ◽  
Dna Repeats ◽  
Centromere Region ◽  
Simple Repeats ◽  
Restriction Fragment Length ◽  
Repeat Induced Point Mutation

ABSTRACT DNA from the centromere region of linkage group (LG) VII ofNeurospora crassa was cloned previously from a yeast artificial chromosome library and was found to be atypical ofNeurospora DNA in both composition (AT rich) and complexity (repetitive). We have determined the DNA sequence of a small portion (∼16.1 kb) of this region and have identified a cluster of three new retrotransposon-like elements as well as degenerate fragments from the 3′ end of Tad, a previously identified LINE-like retrotransposon. This region contains a novel full-length but nonmobilecopia-like element, designated Tcen, that is only associated with centromere regions. Adjacent DNA contains portions of a gypsy-like element designated Tgl1. A third new element, Tgl2, shows similarity to theTy3 transposon of Saccharomyces cerevisiae. All three of these elements appear to be degenerate, containing predominantly transition mutations suggestive of the repeat-induced point mutation (RIP) process. Three new simple DNA repeats have also been identified in the LG VII centromere region. While Tcenelements map exclusively to centromere regions by restriction fragment length polymorphism analysis, the defective Tad elements appear to occur most frequently within centromeres but are also found at other loci including telomeres. The characteristics and arrangement of these elements are similar to those seen in theDrosophila centromere, but the relative abundance of each class of repeats, as well as the sequence degeneracy of the transposon-like elements, is unique to Neurospora. These results suggest that the Neurospora centromere is heterochromatic and regional in character, more similar to centromeres of Drosophila than to those of most single-cell yeasts.

Entire maize chloroplast genome is stably maintained in a yeast artificial chromosome

1991 ◽  
Vol 17 (3) ◽  
pp. 361-369 ◽  
Author(s):  
Manju Gupta ◽  
Brad Hoo
Keyword(s):  
Chloroplast Genome ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Maize Chloroplast

scholarly journals Chromosomal localization of human genes for arylamine N-acetyltransferase

1994 ◽  
Vol 297 (3) ◽  
pp. 441-445 ◽  
Author(s):  
D Hickman ◽  
A Risch ◽  
V Buckle ◽  
N K Spurr ◽  
S J Jeremiah ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
In Situ Hybridization ◽  
Yeast Artificial Chromosome ◽  
Chromosomal Localization ◽  
Artificial Chromosome ◽  
Acetylator Phenotype ◽  
Chromosome 8 ◽  
Slow Acetylator ◽  
Human Genes

Arylamine N-acetyltransferase is encoded at two loci, AAC-1 and AAC-2, on human chromosome 8. The products of the two loci are able to catalyse N-acetylation of arylamine carcinogens, such as benzidine and other xenobiotics. AAC-2 is polymorphic and individuals carrying the slow-acetylator phenotype are more susceptible to benzidine-induced bladder cancer. We have identified yeast artificial chromosome clones encoding AAC-1 and AAC-2 and have used the cloned DNAs as fluorescent probes for in situ hybridization. The hybridization patterns allow assignment of AAC-1 and AAC-2 to chromosome 8p21.3-23.1, a region in which deletions have been associated with bladder cancer [Knowles, Shaw and Proctor (1993) Oncogene 8, 1357-1364].

Compositional heterogeneity and patterns of molecular evolution in the Drosophila genome.

Genetics ◽  
1993 ◽  
Vol 134 (3) ◽  
pp. 837-845
Author(s):  
J P Carulli ◽  
D E Krane ◽  
D L Hartl ◽  
H Ochman
Keyword(s):  
Molecular Evolution ◽  
Base Composition ◽  
Yeast Artificial Chromosome ◽  
Strong Support ◽  
Direct Determination ◽  
Artificial Chromosome ◽  
Significant Heterogeneity ◽  
Sequence Evolution ◽  
Drosophila Genome ◽  
Eukaryotic Organisms

Abstract The rates and patterns of molecular evolution in many eukaryotic organisms have been shown to be influenced by the compartmentalization of their genomes into fractions of distinct base composition and mutational properties. We have examined the Drosophila genome to explore relationships between the nucleotide content of large chromosomal segments and the base composition and rate of evolution of genes within those segments. Direct determination of the G + C contents of yeast artificial chromosome clones containing inserts of Drosophila melanogaster DNA ranging from 140-340 kb revealed significant heterogeneity in base composition. The G + C content of the large segments studied ranged from 36.9% G + C for a clone containing the hunchback locus in polytene region 85, to 50.9% G + C for a clone that includes the rosy region in polytene region 87. Unlike other organisms, however, there was no significant correlation between the base composition of large chromosomal regions and the base composition at fourfold degenerate nucleotide sites of genes encompassed within those regions. Despite the situation seen in mammals, there was also no significant association between base composition and rate of nucleotide substitution. These results suggest that nucleotide sequence evolution in Drosophila differs from that of many vertebrates and does not reflect distinct mutational biases, as a function of base composition, in different genomic regions. Significant negative correlations between codon-usage bias and rates of synonymous site divergence, however, provide strong support for an argument that selection among alternative codons may be a major contributor to variability in evolutionary rates within Drosophila genomes.

scholarly journals Construction of a yeast artificial chromosome contig spanning the spinal muscular atrophy disease gene region.

1993 ◽  
Vol 90 (14) ◽  
pp. 6801-6805 ◽  
Author(s):  
P. W. Kleyn ◽  
C. H. Wang ◽  
L. L. Lien ◽  
E. Vitale ◽  
J. Pan ◽  
...  
Keyword(s):  
Spinal Muscular Atrophy ◽  
Disease Gene ◽  
Yeast Artificial Chromosome ◽  
Muscular Atrophy ◽  
Artificial Chromosome ◽  
Gene Region ◽  
Yeast Artificial Chromosome Contig
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