Elicitation of Xenopus laevis Tadpole and Adult Frog Peritoneal Leukocytes

2017 ◽  
Vol 2018 (7) ◽  
pp. pdb.prot097642 ◽  
Author(s):  
Leon Grayfer
Keyword(s):  
Xenopus Laevis ◽  
Adult Frog ◽  
Peritoneal Leukocytes ◽  
Xenopus Laevis Tadpole

Author response for "Amphibian thalamic nuclear organization during larval development and in the adult frog Xenopus laevis : genoarchitecture and hodological analysis"

2020 ◽  
Author(s):  
Ruth Morona ◽  
Sandra Bandín ◽  
Jesús M. López ◽  
Nerea Moreno ◽  
Agustín González
Keyword(s):  
Xenopus Laevis ◽  
Larval Development ◽  
Nuclear Organization ◽  
Author Response ◽  
Adult Frog

scholarly journals Expression of the Ca2+-binding protein, parvalbumin, during embryonic development of the frog, Xenopus laevis

1987 ◽  
Vol 104 (4) ◽  
pp. 841-847 ◽  
Author(s):  
BK Kay ◽  
AJ Shah ◽  
WE Halstead
Keyword(s):  
Xenopus Laevis ◽  
Fusion Protein ◽  
Binding Protein ◽  
Expression Library ◽  
Simple Method ◽  
High Affinity ◽  
Tadpole Tail ◽  
Developing Muscle ◽  
Beta Galactosidase ◽  
Xenopus Laevis Tadpole

A cDNA segment encoding the Ca2+-binding protein, parvalbumin, was isolated with the use of antibodies, from a lambda gtll expression library of Xenopus laevis tadpole poly(A)+ RNAs. The bacterially expressed beta-galactosidase-parvalbumin fusion protein of one lambda recombinant shows high affinity 45Ca2+ binding. The sequence of the tadpole parvalbumin is highly similar to previously characterized beta-parvalbumins of other organisms. Data from protein and RNA blotting experiments demonstrate that parvalbumin is absent in oocytes, eggs, and early staged embryos, and only becomes expressed during embryogenesis at the time of myogenesis. The protein can be detected in individual developing muscle cells and in muscle fibers of tadpole tail muscles. A simple method is also described for the isolation of neural tube-notochord-somite complexes from Xenopus embryos.

scholarly journals Innate Immune Responses and Permissiveness to Ranavirus Infection of Peritoneal Leukocytes in the Frog Xenopus laevis

2010 ◽  
Vol 84 (10) ◽  
pp. 4912-4922 ◽  
Author(s):  
Heidi D. Morales ◽  
Lara Abramowitz ◽  
Jacqueline Gertz ◽  
Jessica Sowa ◽  
Ashley Vogel ◽  
...  
Keyword(s):  
T Cell ◽  
Immune Responses ◽  
Xenopus Laevis ◽  
T Cell Response ◽  
Necrosis Factor Alpha ◽  
Main Site ◽  
Frog Virus ◽  
Reverse Transcription Pcr ◽  
Arginase 1 ◽  
Peritoneal Leukocytes

ABSTRACT Ranaviruses such as frog virus 3 ([FV3] family Iridoviridae) are increasingly prevalent pathogens that infect reptiles, amphibians, and fish worldwide. Whereas studies in the frog Xenopus laevis have revealed the critical involvement of CD8 T-cell and antibody responses in host resistance to FV3, little is known about the role played by innate immunity to infection with this virus. We have investigated the occurrence, composition, activation status, and permissiveness to infection of peritoneal leukocytes (PLs) in Xenopus adults during FV3 infection by microscopy, flow cytometry, and reverse transcription-PCR. The total number of PLs and the relative fraction of activated mononucleated macrophage-like cells significantly increase as early as 1 day postinfection (dpi), followed by NK cells at 3 dpi, before the peak of the T-cell response at 6 dpi. FV3 infection also induces a rapid upregulation of proinflammatory genes including arginase 1, interleukin-1β, and tumor necrosis factor alpha. Although PLs are susceptible to FV3 infection, as evidenced by apoptotic cells, active FV3 transcription, and the detection of viral particles by electron microscopy, the infection is weaker (fewer infectious particles), more transitory, and involves a smaller fraction (less than 1%) of PLs than the kidney, the main site of infection. However, viral DNA remains detectable in PLs for at least 3 weeks postinfection, past the point of viral clearance observed in the kidneys. This suggests that although PLs are actively involved in anti-FV3 immune responses, some of these cells can be permissive and harbor quiescent, asymptomatic FV3.

Specific cell surface labels in the visual centers of Xenopus laevis tadpole identified using monoclonal antibodies

1987 ◽  
Vol 122 (1) ◽  
pp. 90-100 ◽  
Author(s):  
Shin Takagi ◽  
Toshiaki Tsuji ◽  
Takashi Amagai ◽  
Tetsuro Takamatsu ◽  
Hajime Fujisawa
Keyword(s):  
Monoclonal Antibodies ◽  
Cell Surface ◽  
Xenopus Laevis ◽  
Specific Cell ◽  
Specific Cell Surface ◽  
Xenopus Laevis Tadpole

The sensitivity of Xenopus laevis tadpole tail tissue to the action of thyroid hormones

1977 ◽  
Vol 32 (2) ◽  
pp. 179-186 ◽  
Author(s):  
Helen Robinson ◽  
Sara Chaffee ◽  
Valerie Anne Galton
Keyword(s):  
Xenopus Laevis ◽  
Thyroid Hormones ◽  
Tadpole Tail ◽  
Xenopus Laevis Tadpole

scholarly journals Cloning of Xenopus laevis Surfactant Protein C from Adult Frog Lung

1999 ◽  
Vol 45 (4, Part 2 of 2) ◽  
pp. 60A-60A
Author(s):  
Ernesto R Resnik ◽  
John Wermager ◽  
David N Cornfield
Keyword(s):  
Xenopus Laevis ◽  
Protein C ◽  
Surfactant Protein ◽  
Surfactant Protein C ◽  
Adult Frog
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