Alkaline Agarose Gel Electrophoresis
Keyword(s):
High Ph
◽
Alkaline agarose gels are run at high pH, which causes each thymine and guanine residue to lose a proton and thus prevents the formation of hydrogen bonds with their adenine and cytosine partners. The denatured DNA is maintained in a single-stranded state and migrates through an alkaline agarose gel as a function of its size. Other denaturants such as formamide and urea do not work well because they cause the agarose to become rubbery.
1986 ◽
Vol 158
(1)
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pp. 72-78
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1988 ◽
Vol 60
(02)
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pp. 133-136
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1976 ◽
Vol 17
(3)
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pp. 916-923
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Keyword(s):
Keyword(s):
2012 ◽
Vol 16
◽
pp. e395
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Keyword(s):
1989 ◽
Vol 17
(3)
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pp. 150-151
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Keyword(s):
Preparative Polyacrylamide-Agarose Gel Electrophoresis of Proteins and RNAS by the Use of New Device
1979 ◽
Vol 9
(3)
◽
pp. 303-320
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Keyword(s):
1979 ◽
Vol 91
(3)
◽
pp. 285-294
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