Serum creatine kinase isoenzyme MB activity: Evaluation of a kit employing agarose-gel electrophoresis with overlay paper fluorescence scanning

1979 ◽  
Vol 91 (3) ◽  
pp. 285-294 ◽  
Author(s):  
Stanley R. Hamilton ◽  
Thomas Wimsatt ◽  
Rosemary Torrieri ◽  
Robert C. Rock
Keyword(s):  
Creatine Kinase ◽  
Gel Electrophoresis ◽  
Serum Creatine Kinase ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Creatine Kinase Isoenzyme ◽  
Activity Evaluation ◽  
Creatine Kinase Isoenzyme Mb

Isoenzymes of creatine kinase in extracts of various parts and regions of the human central nervous system.

1980 ◽  
Vol 26 (6) ◽  
pp. 760-762 ◽  
Author(s):  
R R Petronia ◽  
A H Maas ◽  
C W van Veelen ◽  
G E Staal
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Creatine Kinase ◽  
Gel Electrophoresis ◽  
Cauda Equina ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Human Central Nervous System ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Isoenzymes

Abstract The distribution of isoenzymes of creatine kinase (EC 2.7.3.2.) was investigated by agarose gel electrophoresis of extracts of selected parts and regions of the human central nervous system. Besides the major brain isoenzyme BB, we demonstrated the presence of three other creatine kinase isoenzyme forms. The distribution of creatine kinase isoenzymes depending strongly on the region from which the biopsy was taken. We found substantial amounts of the MB isoenzyme in extracts of the dura from the cauda equina of the two adults examined.

Isoenzymes of creatine kinase in extracts of various parts and regions of the human central nervous system.

1980 ◽  
Vol 26 (6) ◽  
pp. 760-762 ◽  
Author(s):  
R R Petronia ◽  
A H Maas ◽  
C W van Veelen ◽  
G E Staal
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Creatine Kinase ◽  
Gel Electrophoresis ◽  
Cauda Equina ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Human Central Nervous System ◽  
Creatine Kinase Isoenzyme ◽  
Creatine Kinase Isoenzymes

Abstract The distribution of isoenzymes of creatine kinase (EC 2.7.3.2.) was investigated by agarose gel electrophoresis of extracts of selected parts and regions of the human central nervous system. Besides the major brain isoenzyme BB, we demonstrated the presence of three other creatine kinase isoenzyme forms. The distribution of creatine kinase isoenzymes depending strongly on the region from which the biopsy was taken. We found substantial amounts of the MB isoenzyme in extracts of the dura from the cauda equina of the two adults examined.

Creatine Kinase Isoenzyme Band Mimicking CK MB on Agarose Gel Electrophoresis

1991 ◽  
Vol 95 (5) ◽  
pp. 753-756
Author(s):  
J. Thomas Hindmarsh ◽  
Donald C. Greenway ◽  
Pok Cheung ◽  
Abid Khodadeen
Keyword(s):  
Creatine Kinase ◽  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Creatine Kinase Isoenzyme

Relative merits of two electrophoretic and two column-chromatographic kets for determining serum creatine kinase isoenzyme MB activity.

1978 ◽  
Vol 24 (11) ◽  
pp. 2013-2017 ◽  
Author(s):  
C Hamlin ◽  
E Ackerman
Keyword(s):  
Creatine Kinase ◽  
Clinical Evaluation ◽  
Serum Creatine Kinase ◽  
Cardiac Monitoring ◽  
Prospective Analysis ◽  
Creatine Kinase Isoenzyme ◽  
Discrepant Result ◽  
Column Chromatographic ◽  
Creatine Kinase Isoenzyme Mb

Abstract Prospective analysis (by use of commercially available kits) of 339 validated admission specimens from patients consecutively admitted to a cardiac monitoring unit revealed sensitivities of 68 and 63% for two electrophoretic assays (Bioware and Helena) and up to 73 and 77% for two chromatographic assays (Roche and Worthington). Specificities were 94.8, 96,6, 90.2, and 89.7% and efficiencies were 90.2, 90.7, 87.0, and 87.0, respectively. Although these results differ from early reports, they agree well with several recent studies. However, 86 (25%) of the specimens gave at least one discrepant result based on clinical evaluation of the patients, and 68 (20%) gave at least one discrepant result between methods.

Transient increase in serum creatine kinase isoenzyme BB activity after prostatectomy in a patient with massive benign prostatic hyperplasia.

1980 ◽  
Vol 26 (3) ◽  
pp. 511-512
Author(s):  
Z D Goodman ◽  
S R Hamilton
Keyword(s):  
Creatine Kinase ◽  
Benign Prostatic Hyperplasia ◽  
Myocardial Injury ◽  
Prostate Gland ◽  
Serum Creatine Kinase ◽  
Prostatic Hyperplasia ◽  
Prostatic Adenocarcinoma ◽  
Agarose Gel Electrophoresis ◽  
Creatine Kinase Isoenzyme ◽  
Prostatic Disease

Abstract The activity of creatine kinase isoenzyme BB (CK-BB) in serum is rarely abnormally high (i.e., detectable). An increase in immunoreactive CK-BB or CK-BB activity in patients with prostatic disease has been proposed as an indication of prostatic adenocarcinoma. Here we report the case of an elderly man with massive benign prostatic hyperplasia but no clinical or pathological evidence of prostatic adenocarcinoma, whose serum CK-BB activity was found by agarose gel electrophoresis to be 1 U/L (normal: 0%), 10% of his total CK activity. Serum CK-BB activity was further increased to 16 U/L (20% of total CK activity) 1 h after prostatectomy, but became undetectable by the second day after the operation. The findings suggest that: (a) the source of the serum CK-BB activity was the enlarged prostate gland; (b) abnormally high CK-BB activity in serum of men with prostatic disease does not necessarily indicate the presence of prostatic adenocarcinoma; and (c) myocardial injury could be erroneously diagnosed postoperatively in prostatectomy patients if CK isoenzyme methods are used that do not consistently separate "heart-specific" CK-MB from CK-BB.

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