Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients: Continuous Gradients

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot3927 ◽  
Author(s):  
Joseph Sambrook ◽  
David W. Russell
Keyword(s):  
Ethidium Bromide ◽  
Circular Dna ◽  
Equilibrium Centrifugation

Purification of Closed Circular DNA by Equilibrium Centrifugation in CsCl-Ethidium Bromide Gradients: Discontinuous Gradients

2006 ◽  
Vol 2006 (1) ◽  
pp. pdb.prot3913
Author(s):  
Joseph Sambrook ◽  
David W. Russell
Keyword(s):  
Ethidium Bromide ◽  
Circular Dna ◽  
Equilibrium Centrifugation

Disappearance of the covalently closed circular cryptic plasmid PO-2 in Salmonella pullorum MS35 containing F77

1974 ◽  
Vol 20 (4) ◽  
pp. 551-557
Author(s):  
Paul W. Stiffler ◽  
D. E. Schoenhard
Keyword(s):  
Salmonella Typhimurium ◽  
Sucrose Gradient ◽  
Buoyant Density ◽  
Sedimentation Coefficient ◽  
Apparent Effect ◽  
Donor Property ◽  
Circular Dna ◽  
Salmonella Pullorum ◽  
Cryptic Plasmids ◽  
Equilibrium Centrifugation

The physical basis of the donor property of Salmonella pullorum donor strains MS8300, MS830, and MS831 carrying the F77 factor from Salmonella typhimurium was investigated by dye-buoyant density equilibrium centrifugation and zonal centrifugation in neutral sucrose gradients. Centrifugation of the MS8300 and MS831 closed circular DNA material in a 20 to 31% neutral sucrose gradient resulted in a profile having one sharp peak of radioactivity with a sedimentation coefficient of 17 S and a broad peak extending from 65 to 70 S. The 17- and 65-S species were isolated from the isogenic F− strain MS83. These appeared identical with those isolated previously in S. pullorum MS53 as the cryptic plasmids PO-1 and PO-2 respectively. Cosedimentation of differentially labeled F77 DNA and the lysate containing the 65-S and 70-S species suggested that the 70-S species is the autonomous F77 factor in strains MS8300 and MS831. Lysates of MS830 similarly treated produced a profile containing the 17-S molecule and possibly some 70-S molecules but no 65-S molecules. It was concluded that the F77 factor was integrated in most cells and that the covalently closed circular state of PO-2 plasmid was lost. The mutation in the cysE gene of the F77 factor carried by MS831 had no apparent effect on the covalently closed circular nature of PO-2 plasmid, although F77 no longer seemed to mobilize the chromosome from the cysE locus.

The use of ethidium bromide-circular DNA complexes for the fluorometric analysis of breakage and joining of DNA

1971 ◽  
Vol 55 (1) ◽  
pp. 75-100 ◽  
Author(s):  
C. Paoletti ◽  
J.B. LePecq ◽  
I.R. Lehman
Keyword(s):  
Ethidium Bromide ◽  
Dna Complexes ◽  
Circular Dna ◽  
Fluorometric Analysis

Restricted uptake of ethidium bromide and propidium diiodide by denatured closed circular DNA in buoyant cesium chloride

1974 ◽  
Vol 86 (2) ◽  
pp. 271-283 ◽  
Author(s):  
Lawrence I. Grossman ◽  
Robert Watson ◽  
Jerome Vinograd
Keyword(s):  
Ethidium Bromide ◽  
Cesium Chloride ◽  
Circular Dna

Effect of ethidium bromide on transcription of linear and circular DNA templates

1973 ◽  
Vol 78 (4) ◽  
pp. 715-720 ◽  
Author(s):  
John P. Richabdson ◽  
Stephen R. Pabker
Keyword(s):  
Ethidium Bromide ◽  
Dna Templates ◽  
Circular Dna ◽  
Linear And Circular Dna

Ethidium-bromide-inhibited restriction endonucleases cleave one strand of circular DNA

Gene ◽  
1982 ◽  
Vol 20 (1) ◽  
pp. 121-125 ◽  
Author(s):  
Mårten Österlund ◽  
Holger Luthman ◽  
Stefan V. Nilsson ◽  
Göran Magnusson
Keyword(s):  
Ethidium Bromide ◽  
Restriction Endonucleases ◽  
Circular Dna

scholarly journals Densitometric quantification of DNA topoisomers in ethidium bromide-stained agarose gels and chemiluminescence-detected X ray films.

2000 ◽  
Vol 47 (3) ◽  
pp. 835-839 ◽  
Author(s):  
Z Topcu
Keyword(s):  
Ethidium Bromide ◽  
Band Intensity ◽  
Uv Exposure ◽  
Circular Dna ◽  
X Ray ◽  
Agarose Gels ◽  
Southern Blots ◽  
Plasmid Dnas ◽  
Circular Plasmid ◽  
The Relationship

This study investigates the contribution of deformational strain imposed by topological interconversions of DNA in ethidium bromide-binding on agarose gels. Closed-circular plasmid DNAs were nicked using UV exposure and the DNA bands were quantified by densitometry. The results show that the closed circular DNA binds the same amount of the dye as its nicked counterpart. The relationship between the band intensity on X-ray films of chemiluminescence-detected Southern blots and DNA concentration was shown to be linear.

scholarly journals Differential Effect of Ethidium Bromide and Cytosine Arabinoside on Mitochondrial and Nuclear DNA Synthesis in HeLa Cells

1972 ◽  
Vol 27 (8) ◽  
pp. 989-991 ◽  
Author(s):  
Kenzo Kato ◽  
Klaus D. Radsak ◽  
Hilary Koprowski
Keyword(s):  
Hela Cell ◽  
Dna Synthesis ◽  
Ethidium Bromide ◽  
Hela Cells ◽  
Cytosine Arabinoside ◽  
Differential Effect ◽  
Mammalian Cells ◽  
Nuclear Dna ◽  
Circular Dna ◽  
Isopycnic Centrifugation

The effect of ethidium bromide (EB) on the synthesis of circular DNA of mammalian cells was studied by isopycnic centrifugation in a CsCl-EB solution. EB (0.1—0.5 μg/ml) interferes with the synthesis of newly-formed circular DNA of HeLa cell mitochondria and causes degradation of the pre-existing circular DNA, as well. Under the same conditions, nuclear DNA synthesis was not inhibited. This effect was not reversible at a concentration of 0.5 μg EB/ml or more. Cytosine arabinoside (ara-C) did not exhibit an effect similar to that of EB.

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