Atomic Force Microscopy for Biological Imaging and Mechanical Testing across Length Scales

2010 ◽  
Vol 2010 (10) ◽  
pp. pdb.top86-pdb.top86 ◽  
Author(s):  
M. Plodinec ◽  
M. Loparic ◽  
U. Aebi
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Testing ◽  
Biological Imaging ◽  
Length Scales ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals A Computational Framework to Model Mesenchymal Stem Cell Nucleus Mechanics Using Confocal Microscopy

2020 ◽  
Author(s):  
Zeke Kennedy
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Testing ◽  
Cell Nucleus ◽  
Mechanical Stimuli ◽  
Computational Framework ◽  
Conversion Factors ◽  
Testing Apparatus ◽  
Force Microscopy ◽  
Atomic Force ◽  
Confocal Images

The mechanical properties of the cell nucleus are emerging as a key component in genetic transcription. It has been shown that the stiffness of the nucleus in part regulates the transcription of genes in response to external mechanical stimuli. The stiffness has been shown to change as a result of both disease and changes to the external environment. While the mechanical structure of the nucleus can be visually documented using a confocal microscope, it is currently impossible to test the stiffness of the nucleus without a mechanical testing apparatus such as an atomic force microscope. This is problematic in that the use of a mechanical testing apparatus involves deconstructing the cell in order to isolate the nucleus and is unable to provide data on internal heterochromatin dynamics within the nucleus. Therefore, our research focused on developing a computational framework that would allow researchers to model the mechanical contributions of the nucleus specific geometry and material dispersion of both chromatin and LaminA/C within an individual nucleus in order to improve the ability of researchers to study the nucleus. We began by developing a procedure that could generate a finite element geometry of a nucleus using confocal images. This procedure was then utilized to generate models that contained elasticity values that corresponded to the voxel intensities of images of both chromatin and LaminA/C by using a set of conversion factors to link image voxel intensity to model stiffness. We then tuned these conversion factors by running in silico atomic force microscopy experiments on these models while comparing the simulation results to atomic force microscopy data from real world nuclei. From this experiment we were able to find a set of conversion factors that allowed us to replicate the external response of the nucleus. Our developed computational framework will allow future researchers to study the contribution of multitude of sub-nuclear structures and predict global nuclear stiffness of multiple nuclei based on confocal images and AFM tests.

High flexibility of DNA on short length scales probed by atomic force microscopy

2006 ◽  
Vol 1 (2) ◽  
pp. 137-141 ◽  
Author(s):  
Paul A. Wiggins ◽  
Thijn van der Heijden ◽  
Fernando Moreno-Herrero ◽  
Andrew Spakowitz ◽  
Rob Phillips ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Short Length ◽  
Length Scales ◽  
Force Microscopy ◽  
Atomic Force ◽  
High Flexibility

Cryo Atomic Force Microscopy: A New Approach for Biological Imaging at High Resolution

Biochemistry ◽  
1995 ◽  
Vol 34 (26) ◽  
pp. 8215-8220 ◽  
Author(s):  
Wenhai Han ◽  
Jianxun Mou ◽  
Jun Sheng ◽  
Jie Yang ◽  
Zhifeng Shao
Keyword(s):  
Atomic Force Microscopy ◽  
High Resolution ◽  
Biological Imaging ◽  
New Approach ◽  
Force Microscopy ◽  
Atomic Force

Atomic force microscopy and mechanical testing of bovine pericardium irradiated to radiotherapy doses

2014 ◽  
Vol 96 ◽  
pp. 176-180 ◽  
Author(s):  
Eman Daar ◽  
W. Kaabar ◽  
E. Woods ◽  
C. Lei ◽  
A. Nisbet ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Mechanical Testing ◽  
Bovine Pericardium ◽  
Force Microscopy ◽  
Atomic Force

Multiscale Roughness of MEMS Surfaces

2004 ◽  
Author(s):  
Can K. Bora ◽  
Michael E. Plesha ◽  
Erin E. Flater ◽  
Mark D. Street ◽  
Robert W. Carpick ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Power Law ◽  
Multiscale Model ◽  
Length Scales ◽  
Microelectromechanical System ◽  
Force Microscopy ◽  
Atomic Force ◽  
Multiple Length Scales ◽  
Function Approximations

Investigation of contact and friction at multiple length scales is necessary for the design of surfaces in sliding microelectromechanical system (MEMS). A method is developed to investigate the geometry of asperities at different length scales. Analysis of density, height, and curvature of asperities on atomic force microscopy (AFM) images of actual silicon MEMS surfaces show these properties have a power law relationship with the sampling size used to define an asperity. This behavior and its similarity to results for fractal Weierstrass-Mandelbrot (W-M) function approximations indicate that a multiscale model is required to properly describe the surfaces.

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