With parameters fixed by critical temperature and equation of state at zero baryon chemical potential, a realistic Polyakov-Nambu-Jona-Lasinio (rPNJL) model predicts a critical end point of chiral phase transition at (μEB = 720MeV; TE = 93MeV). The extracted freeze-out line from heavy ion collisions is close to the chiral phase transition boundary in the rPNJL model, and the kurtosis kσ2 of baryon number fluctuations from the rPNJL model along the experimental freeze-out line agrees well with the BES-I measurement. Our analysis shows that the dip structure of measured kσ2 is determined by the relationship between the freeze-out line and chiral phase transition line at low baryon density region, and the peak structure can be regarded as a clean signature for the existence of CEP.