scholarly journals Comparison of in Vivo and in Vitro Assays of Nitrate Reductase in Wheat (Triticum aestivum L.) Seedlings

1976 ◽  
Vol 58 (4) ◽  
pp. 583-587 ◽  
Author(s):  
Nichola Brunetti ◽  
Richard H. Hageman

1981 ◽  
Vol 19 (3-4) ◽  
pp. 263-276 ◽  
Author(s):  
P. W. Jones ◽  
W. J. Whittington ◽  
C. B. Johnson




Author(s):  
Rizwan Sheirdil ◽  
Rifat Hayat ◽  
Xiao-Xia Zhang ◽  
Nadeem Abbasi ◽  
Safdar Ali ◽  
...  

Plant growth promoting rhizobacteria (PGPR) are capable to reduce the use of chemical fertilizers input cost of farmer. Keeping in view the study was designed to investigate and evaluate inoculation effect of indigenous rhizospheric bacteria on growth and yield of wheat (Triticum aestivum L.) under in vitro and in vivo conditions using different treatments. Ten potential strains were selected on the basis of their ACC deaminase activity, siderophore production, P-solubilization and production of indole acetic acid (IAA). Further these strains were tested in three different experiments (growth chamber, pot and field). We found significant increase in crop growth response to the inoculants in comparison with un-inoculated control. In pot and field trial we tested PGPR with recommended dose of inorganic fertilizers. The results of present study revealed that inoculation of bacterial strains with wheat seeds significantly increased plant growth and improved crop yield. Results of present study reveal that these strains could be employed in different combinations and can get higher yield in case of half recommended doses of inorganic fertilizers along with consortium of strains in comparison with sole application of recommended dose of fertilizer and with consortium of strains. These strains were further identified by 16Sr RNA gene sequencing, fatty acid profile and biolog. It can be concluded that inoculated bacteria have more potential and contributes in good crop quality, increased yield when they are applied in combination, thus have potential to minimize use of chemical fertilizers.



2006 ◽  
Vol 33 (7) ◽  
pp. 685 ◽  
Author(s):  
Aravind K. Jukanti ◽  
Phil L. Bruckner ◽  
Andreas M. Fischer

Polyphenol oxidases (PPOs) have been implicated in plant defence reactions. From an applied point of view, high PPO activity is associated with browning / darkening of fresh and processed food. Owing to its complex genome and economic importance, wheat (Triticum aestivum L.) represents an interesting system to advance our understanding of plant PPO function. We have previously shown that wheat PPOs are organised in a multigene family, consisting of two distinct phylogenetic clusters with three members each. In this study, we demonstrate that members of one cluster are not expressed in developing kernels or senescing flag leaves. Transcriptional regulation of one major gene in the other cluster largely controls PPO levels in these tissues, at least in the wheat varieties used for this study. Our data further indicate that the product of this gene is present as a latent enzyme during early kernel development, and that the latent enzyme is activated during later developmental phases. Enzyme activation can be achieved in vitro by limited tryptic digestion, but our data do not indicate activation by a proteolytic mechanism in vivo. Together, results presented in this study provide important insights into the regulation of wheat PPO function.





1980 ◽  
Vol 65 (1) ◽  
pp. 27-32 ◽  
Author(s):  
Richard H. Hageman ◽  
Andrew J. Reed ◽  
Rise A. Femmer ◽  
Joseph H. Sherrard ◽  
Michael J. Dalling


Genome ◽  
1987 ◽  
Vol 29 (5) ◽  
pp. 689-694 ◽  
Author(s):  
Yang Yen ◽  
Dajun Liu

Elymus canadensis L. (2n = 28, SSHH), E. dahuricus Turcz. ex Griseb. (2n = 42, SSHH??), and E. cylindricus (Franch.) Honda (2n = 42, SSHH??) were successfully hybridized with Triticum aestivum L. cv. Chinese Spring (2n = 42, AABBDD) by in vivo – in vitro embryo culture. The F1 hybrids were male sterile and morphologically intermediate to their parents. The average numbers of associated chromosome arms per cell were 0.24, 2.04, and 0.65, and the mean arm association frequencies were 0.0086, 0.0486, and 0.0155, respectively. The first backcross derivatives all resulted from fusion of an euploid, hypoploid, or hyperploid unreduced F1 female gamete with a normal male gamete of common wheat. They had average numbers of associated arms per cell of 27.10, 24.36, and 23.84, and average chromosome arm association frequencies of 0.644, 0.580, and 0.568, respectively. First backcross derivatives were male sterile but morphologically, closer to their wheat parents. This study shows that no homologous genomes exist between Elymus and Triticum and that gene transfer between these two genera would be very difficult but not impossible. Key words: cytogenetics, Elymus L., Triticum L., hybrid (intergeneric).



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